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[190141_s_at] tubulin alpha subunit

Description T28D6.2 /REP_DB=WormBase Gene ID /WP=CE16521 /TR=O18154 /GB=CAB03447.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=tubulin alpha subunit
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 60      6grp_total > 34      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 34      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 485
1 2 3 4 range
A 921.3
(3)
P 		487.8
(-3)
P 		555
(-2.1)
P 		604.2
(-1.4)
P 		434
B 870.1
(3)
P 		606.6
(-3)
P 		653.1
(-1.9)
P 		624.9
(-2.6)
P 		264
C 973.2
(3)
P 		509.5
(-2.7)
P 		488
(-3)
P 		555.7
(-2.2)
P 		485
avr 921.5
(3) 		534.6
(-3) 		565.4
(-2.5) 		594.9
(-2.1) 		387

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 474
1 2 3 4 5 6 range
A 562.8
(3)
P 		418
(0.8)
P 		540.3
(2.7)
P 		232.2
(-2)
P 		165.8
(-3)
P 		250.1
(-1.7)
P 		397
B 624.7
(3)
P 		497.3
(1.1)
P 		601.3
(2.6)
P 		345.3
(-1.2)
P 		226.1
(-3)
P 		264.8
(-2.4)
P 		399
C 557.1
(3)
P 		517.6
(2.4)
P 		441.9
(1.3)
P 		300.2
(-0.8)
P 		150.5
(-3)
P 		239
(-1.7)
P 		407
avr 581.5
(3) 		477.6
(1.4) 		527.8
(2.2) 		292.6
(-1.3) 		180.8
(-3) 		251.3
(-1.9) 		401
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 247
1 2 3 4 range
A 335.5
(3)
P 		105.9
(-2.6)
P 		145.1
(-1.6)
P 		88.4
(-3)
P 		247
B 277.5
(3)
P 		149.8
(-2.3)
P 		151.3
(-2.2)
P 		133.2
(-3)
P 		144
C 322.1
(3)
P 		146.5
(-1.6)
P 		170.1
(-1)
P 		93.7
(-3)
P 		228
avr 311.7
(3) 		134.1
(-2.2) 		155.5
(-1.5) 		105.1
(-3) 		207

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 103.8
(0.3)
P 		148.7
(3)
P 		117.7
(1.1)
P 		58.9
(-2.5)
P 		50
(-3)
P 		60.9
(-2.3)
P 		99
B 217.4
(3)
P 		165.5
(0.5)
P 		146
(-0.4)
P 		91.5
(-3)
P 		91.9
(-3)
P 		98.7
(-2.7)
P 		126
C 206.5
(3)
P 		157.9
(1.2)
P 		112.5
(-0.6)
P 		60.4
(-2.6)
P 		48.7
(-3)
P 		80
(-1.8)
P 		158
avr 175.9
(3) 		157.4
(2) 		125.4
(0.3) 		70.3
(-2.6) 		63.5
(-3) 		79.9
(-2.1) 		112
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190141_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6801
Sequence Type Exemplar sequence
Representative Public ID T28D6.2 NCBI
Target Description T28D6.2 /REP_DB=WormBase Gene ID /WP=CE16521 /TR=O18154 /GB=CAB03447.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=tubulin alpha subunit
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067062(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067062 NCBI Caenorhabditis elegans TuBulin, Alpha family member (tba-7) (tba-7) mRNA, complete cds. 11/11 None
SNAP00000023472 ENSEMBL cdna:SNAP chromosome:CEL140:III:11360027:11371651:-1 11/11 None
GENEFINDER00000023478 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11368310:11371651:-1 11/11 None
T28D6.2 ENSEMBL cdna:known chromosome:CEL140:III:11368306:11371651:-1 gene:T28D6.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11368305-11371647(-) 100.0 100.0
Public Domain and Genome References
Gene Title tubulin alpha subunit
Gene Symbol tba-7
Ensembl T28D6.2
Entrez Gene 176568 Entrez gene
SwissProt O18154 EMBL-EBI
WormBase CE16521 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03447 Hypothetical protein T28D6.2 [Caenorhabditis elegans] ref|NP_499463.1| TuBulin, Alpha family member (tba-7) [Caenorhabditis elegans] 0.0
blast CAE67657 Hypothetical protein CBG13220 [Caenorhabditis briggsae] 0.0
blast CAB16856 Hypothetical protein C47B2.3 [Caenorhabditis elegans] ref|NP_001021050.1| C47B2.3 [Caenorhabditis elegans] dbj|BAA03610.1| alpha-2 tubulin [Caenorhabditis elegans] sp|P34690|TBA2_CAEEL Tubulin alpha-2 chain 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 1.5E-103
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 4.3E-73
Sequence
Target Sequence 
>C. ELEGANS:190141_S_AT
gaatttcaaaccaatctggtcccctacccgagaatccacttcccccttgtcgcctacaat
ccactgatctcttcagcccaccacgagttcctatcagttgatgagataacgaaaagctgc
tttgaagcctcaaatcaaatggtaaaatgtgacccgaagcacgggaagtatatggctgta
tgtttactgtatcgtggagacgttgtgccgaaggatgtgtcggctgcgataaaatcgatc
aaaactcagagatccgtgcagtttgtggactggtgtccgactgggtttaaagttggattg
aactatcagccgcctacagtaatcccgggtggggatatggcaaaggttccacgggcggta
tgtatgttgtcgaatactacggcaattgcggaggcgtggcagagattggactataagttt
gatttgatgtatgccaagagagcttttgtgcattggtacgttggcgagggaatggaggaa
ggcgagttctccgaggcacgtgaagatctggctgctttggaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTTCAAACCAATCTGGTCCCCT 332 325 763 Antisense
CCACCACGAGTTCCTATCAGTTGAT 286 273 840 Antisense
GTATCGTGGAGACGTTGTGCCGAAG 383 455 951 Antisense
ACTCAGAGATCCGTGCAGTTTGTGG 375 101 1006 Antisense
TGGACTGGTGTCCGACTGGGTTTAA 282 547 1028 Antisense
GAACTATCAGCCGCCTACAGTAATC 402 349 1062 Antisense
AAGGTTCCACGGGCGGTATGTATGT 497 159 1105 Antisense
ACTACGGCAATTGCGGAGGCGTGGC 638 101 1138 Antisense
GAGCTTTTGTGCATTGGTACGTTGG 206 369 1202 Antisense
GAGTTCTCCGAGGCACGTGAAGATC 176 399 1246 Antisense
CGTGAAGATCTGGCTGCTTTGGAGA 629 243 1261 Antisense
Sequence Source Affymetrix Proprietary Database