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[190131_s_at] enoyl-CoA hydratase

Description F01G10.2 /REP_DB=WormBase Gene ID /WP=CE09164 /TR=O17761 /GB=CAB02891.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=enoyl-CoA hydratase
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 66      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 84      6grp_total > 57      4grp_total > 0      6grp_highest_sub > Cu      6grp_cgc > 37      6grp_high1_sub > Da      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 544
1 2 3 4 range
A 415.1
(-0.7)
P
228.2
(-3)
P
330.9
(-1.7)
P
720.9
(3)
P
493
B 370.6
(-1.6)
P
259.5
(-3)
P
319.9
(-2.3)
P
750.9
(3)
P
491
C 363
(-1.8)
P
263
(-3)
P
501.2
(-0.2)
P
771.7
(3)
P
509
avr 382.9
(-1.4)
250.2
(-3)
384
(-1.4)
747.8
(3)
498

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 763
1 2 3 4 5 6 range
A 568.7
(-0.4)
P
407.1
(-2.3)
P
671.8
(0.9)
P
554.5
(-0.5)
P
349.5
(-3)
P
850.1
(3)
P
501
B 661.1
(-0.4)
P
403.8
(-3)
P
594.4
(-1)
P
448.4
(-2.5)
P
454.1
(-2.5)
P
988
(3)
P
584
C 608.5
(-1.1)
P
482.9
(-2.1)
P
521.1
(-1.8)
P
646.8
(-0.8)
P
371.8
(-3)
P
1112.3
(3)
P
741
avr 612.8
(-0.8)
431.3
(-2.6)
595.8
(-0.9)
549.9
(-1.4)
391.8
(-3)
983.5
(3)
592
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 151.2
(3)
P
49.5
(-3)
P
86.5
(-0.8)
P
105.5
(0.3)
P
102
B 118.2
(0.4)
P
64.1
(-3)
P
74.1
(-2.4)
P
160
(3)
P
96
C 120.2
(-0.3)
P
75.6
(-3)
P
174.7
(3)
P
130.1
(0.3)
P
99
avr 129.9
(2.8)
63.1
(-3)
111.8
(1.2)
131.9
(3)
69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 268
1 2 3 4 5 6 range
A 104.8
(-3)
P
144.8
(-0.7)
P
146.3
(-0.6)
P
140.6
(-0.9)
P
105.5
(-3)
P
207
(3)
P
102
B 230
(-0.3)
P
134.4
(-2.6)
P
144.3
(-2.4)
P
118.8
(-3)
P
184.5
(-1.4)
P
368.4
(3)
P
250
C 225.6
(-0.5)
P
147.3
(-2.4)
P
132.6
(-2.7)
P
130.1
(-2.8)
P
120.3
(-3)
P
372.4
(3)
P
252
avr 186.8
(-1.2)
142.2
(-2.6)
141.1
(-2.6)
129.8
(-3)
136.8
(-2.8)
315.9
(3)
186
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190131_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8553
Sequence Type Exemplar sequence
Representative Public ID F01G10.2 NCBI
Target Description F01G10.2 /REP_DB=WormBase Gene ID /WP=CE09164 /TR=O17761 /GB=CAB02891.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=enoyl-CoA hydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069475(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_069475 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-8) (ech-8) mRNA, complete cds. 11/11 None
SNAP00000013159 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10236670:10239085:-1 11/11 None
GENEFINDER00000013169 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10236670:10239085:-1 11/11 None
F01G10.2 ENSEMBL cdna:known chromosome:CEL140:IV:10236613:10239085:-1 gene:F01G10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10236675-10239091(-) 100.0 100.0
Public Domain and Genome References
Gene Title enoyl-CoA hydratase
Gene Symbol ech-8
Ensembl F01G10.2
Entrez Gene 177905 Entrez gene
SwissProt O17761 EMBL-EBI
WormBase CE35450 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6631 fatty acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02891 Hypothetical protein F01G10.2 [Caenorhabditis elegans] ref|NP_501876.2| Enoyl-CoA Hydratase family member (ech-8) [Caenorhabditis elegans] 0.0
blast CAE59903 Hypothetical protein CBG03387 [Caenorhabditis briggsae] 0.0
blast CAE59904 Hypothetical protein CBG03388 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 4.9E-15
Pfam IPR006176 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain 9.6E-69
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 4.9E-15
Pfam IPR006176 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain 2.0E-15
Pfam IPR006176 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain 1.1E-42
Sequence
Target Sequence
>C. ELEGANS:190131_S_AT
gaatacggatatcttcctcatcaaattgataaaatcattacaaactttggattcctaatg
ggtcctttaacagtagctgatatgaatggattagatgtaatggaaaagttgaaaaaggag
aatggatggccagcaagtgattttgaaaaggaagtttggagacaaaagagatacggtaga
aaaacaaataagggatactacaaatatgatccaaatactcacaaaaaggagaatgatata
gaaatggagcagctcattcgaaaattttcttcagctgcaagtccaaatatacaaattttg
aacgatcaggatgccgtcaatttcctcttgtatcccatggtcaacgaaggtcttttgtgc
attgaggaaggaatcatccatcatgaaagtttgatcgatatcatgttcattctcgggttt
ggatggcctgttgctactggtggtccgatgagatttggatggcttcaaggaattgaaaaa
gttgcgagcacaattattctctggaattctttagaaccaaatgatagaacatacaaagtt
gcggccacgttgc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GAATACGGATATCTTCCTCATCAAA 387 327 829 Antisense
GATTCCTAATGGGTCCTTTAACAGT 624 431 878 Antisense
ATGGAGCAGCTCATTCGAAAATTTT 169 53 1072 Antisense
AATTTTCTTCAGCTGCAAGTCCAAA 91 175 1091 Antisense
TCAGGATGCCGTCAATTTCCTCTTG 398 623 1134 Antisense
TCCTCTTGTATCCCATGGTCAACGA 200 591 1151 Antisense
GGTCAACGAAGGTCTTTTGTGCATT 598 503 1167 Antisense
CTGTTGCTACTGGTGGTCCGATGAG 428 237 1256 Antisense
GTCCGATGAGATTTGGATGGCTTCA 101 477 1271 Antisense
GTTGCGAGCACAATTATTCTCTGGA 702 439 1309 Antisense
ACATACAAAGTTGCGGCCACGTTGC 639 101 1357 Antisense
Sequence Source Affymetrix Proprietary Database