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[190101_s_at]

Description R151.5 /REP_DB=WormBase Gene ID /WP=CE27200 /GEN=toh-2 /GB=AAK29859.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Zinc metalloprotease
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 64      6grp_total > 63      4grp_total > 0      6grp_highest_sub > 03_04      6grp_cgc > 76      6grp_high1_sub > 03_04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 438
1 2 3 4 range
A 625.8
(-0.8)
P 		463.6
(-3)
P 		634.6
(-0.7)
P 		902
(3)
P 		438
B 605.3
(-3)
P 		606
(-3)
P 		679.4
(0)
P 		752.2
(3)
P 		147
C 622.8
(-1.9)
P 		567.6
(-3)
P 		746.5
(0.6)
P 		862.1
(3)
P 		295
avr 618
(-1.5) 		545.7
(-3) 		686.8
(-0.1) 		838.8
(3) 		293

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 437
1 2 3 4 5 6 range
A 617.3
(-1.6)
P 		529.5
(-3)
P 		846.5
(2.2)
P 		893.1
(3)
P 		646
(-1.1)
P 		709.2
(-0)
P 		364
B 739.1
(-0.5)
P 		573
(-3)
P 		966.6
(3)
P 		850.5
(1.2)
P 		718
(-0.8)
P 		728
(-0.6)
P 		394
C 586.8
(-3)
P 		633.4
(-2)
P 		796.8
(1.4)
P 		872.4
(3)
P 		639.5
(-1.9)
P 		775.8
(1)
P 		286
avr 647.7
(-1.6) 		578.6
(-3) 		870
(3) 		872
(3) 		667.8
(-1.2) 		737.7
(0.3) 		293
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 227.8
(3)
P 		100.6
(-3)
P 		165.9
(0.1)
P 		132
(-1.5)
P 		127
B 193
(3)
P 		149.7
(-3)
P 		157.4
(-1.9)
P 		160.3
(-1.5)
P 		43
C 206.2
(0.2)
P 		163.2
(-2.1)
P 		260.2
(3)
P 		145.4
(-3)
P 		115
avr 209
(3) 		137.8
(-3) 		194.5
(1.8) 		145.9
(-2.3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 113.8
(-3)
P 		188.3
(1)
P 		184.3
(0.8)
P 		226.5
(3)
P 		194.9
(1.3)
P 		172.7
(0.1)
P 		113
B 257.2
(1)
P 		190.7
(-3)
P 		234.7
(-0.4)
P 		225.3
(-0.9)
P 		291.7
(3)
P 		271.5
(1.8)
P 		101
C 217.5
(-0)
P 		193.3
(-1.7)
P 		202.8
(-1)
P 		175.4
(-3)
P 		206.9
(-0.8)
P 		259.7
(3)
P 		84
avr 196.2
(-2.3) 		190.8
(-3) 		207.3
(-0.7) 		209.1
(-0.5) 		231.2
(2.5) 		234.6
(3) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190101_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6187
Sequence Type Exemplar sequence
Representative Public ID R151.5 NCBI
Target Description R151.5 /REP_DB=WormBase Gene ID /WP=CE27200 /GEN=toh-2 /GB=AAK29859.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027561(11), NM_001027560(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027561 NCBI Caenorhabditis elegans TOllisH (Tolloid and BMP-1 family) family member (toh-2) (toh-2) mRNA, complete cds. 11/11 None
NM_001027560 NCBI Caenorhabditis elegans TOllisH (Tolloid and BMP-1 family) family member (toh-2) (toh-2) mRNA, complete cds. 11/11 None
GENEFINDER00000037943 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7202586:7207437:1 10/11 None
R151.5a ENSEMBL cdna:known chromosome:CEL140:III:7202585:7207555:1 gene:R151.5 11/11 A 
R151.5b ENSEMBL cdna:known chromosome:CEL140:III:7202586:7207572:1 gene:R151.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000037932 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7202583-7207842(+) 99.84 100.0
Public Domain and Genome References
Ensembl R151.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAT92065 Tollish (tolloid and bmp-1 family) protein 2, isoform b [Caenorhabditis elegans] ref|NP_001022732.1| TOllisH (Tolloid and BMP-1 family) family member (toh-2) [Caenorhabditis elegans] 0.0
blast AAK29859 Tollish (tolloid and bmp-1 family) protein 2, isoform a [Caenorhabditis elegans] ref|NP_001022731.1| TOllisH (Tolloid and BMP-1 family) family member (toh-2) [Caenorhabditis elegans] sp|P98060|NAS35_CAEEL Zinc metalloproteinase nas-35 precursor (Nematode astacin 35) (Dumpy protein 31) (Tollish protein 2) 0.0
blast T16761 hypothetical protein R151.5 - Caenorhabditis elegans 0.0
blast CAE70154 Hypothetical protein CBG16621 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 6.6E-112
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 3.1E-13
Pfam IPR000859 EMBL-EBI CUB 1.3E-17
Sequence
Target Sequence 
>C. ELEGANS:190101_S_AT
tgcaatactcaagcttgtacgggtatgtgcgctcagaaacgggaggaagagggtcaatgt
ggaggtttcctttctttgttacgaggcgtccgttgtagacaagagaagacggtgatggct
ccatgcgagaacgcgtgctgtccaggtttcactcttcagcgtggcagatgtgtccggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCAATACTCAAGCTTGTACGGGTA 616 575 1612 Antisense
TGTACGGGTATGTGCGCTCAGAAAC 98 555 1627 Antisense
GGGTCAATGTGGAGGTTTCCTTTCT 189 493 1662 Antisense
TCCTTTCTTTGTTACGAGGCGTCCG 75 591 1679 Antisense
GTTACGAGGCGTCCGTTGTAGACAA 489 445 1689 Antisense
GAGAAGACGGTGATGGCTCCATGCG 37 393 1714 Antisense
GATGGCTCCATGCGAGAACGCGTGC 235 407 1725 Antisense
CATGCGAGAACGCGTGCTGTCCAGG 109 211 1733 Antisense
TGCTGTCCAGGTTTCACTCTTCAGC 128 583 1747 Antisense
TTTCACTCTTCAGCGTGGCAGATGT 334 673 1758 Antisense
CTTCAGCGTGGCAGATGTGTCCGGT 493 221 1765 Antisense
Sequence Source Affymetrix Proprietary Database