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[190100_at]

Description R151.5 /REP_DB=WormBase Gene ID /WP=CE27200 /GEN=toh-2 /GB=AAK29859.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Zinc metalloprotease
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 82      4grp_highest_8_8 > 28      4grp_high_8_8 > 0      6grp_highest_nonorm > 74      6grp_total > 80      4grp_total > 07      6grp_highest_sub > 04      6grp_cgc > 96      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 212
1 2 3 4 range
A 568.5
(-3)
P 		626.9
(1.4)
P 		586.4
(-1.7)
P 		648.1
(3)
P 		80
B 625.6
(1.4)
P 		477.9
(-3)
P 		646.8
(2.1)
P 		678.5
(3)
P 		201
C 573.5
(1.8)
P 		466.2
(-3)
P 		599.8
(3)
P 		526.2
(-0.3)
P 		134
avr 589.2
(1.2) 		523.7
(-3) 		611
(2.6) 		617.6
(3) 		94

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 420
1 2 3 4 5 6 range
A 706.9
(-1.5)
P 		639.8
(-3)
P 		651
(-2.7)
P 		906.3
(3)
P 		696.5
(-1.7)
P 		657.6
(-2.6)
P 		267
B 668.5
(-0.4)
P 		530.4
(-2.4)
P 		488.9
(-3)
P 		898
(3)
P 		738
(0.7)
P 		614.9
(-1.2)
P 		409
C 641.2
(-1.6)
P 		622
(-1.9)
P 		746.4
(0.2)
P 		909.1
(3)
P 		655.1
(-1.4)
P 		560.8
(-3)
P 		348
avr 672.2
(-1.5) 		597.4
(-3) 		628.8
(-2.4) 		904.5
(3) 		696.5
(-1.1) 		611.1
(-2.7) 		307
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 207
(3)
P 		136.1
(-0.8)
P 		153.3
(0.1)
P 		94.9
(-3)
P 		112
B 199.5
(3)
P 		118
(-3)
P 		149.9
(-0.7)
P 		144.6
(-1)
P 		82
C 189.8
(2)
P 		134
(-0.7)
P 		209
(3)
P 		88.7
(-3)
P 		120
avr 198.8
(3) 		129.4
(-1.7) 		170.7
(1.1) 		109.4
(-3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 181
1 2 3 4 5 6 range
A 130.3
(-3)
P 		227.5
(2.9)
P 		141.8
(-2.3)
P 		229.9
(3)
P 		210.2
(1.8)
P 		160.1
(-1.2)
P 		100
B 232.6
(0.8)
P 		176.5
(-1.1)
P 		118.7
(-3)
P 		237.8
(0.9)
P 		299.8
(3)
P 		229.3
(0.7)
P 		181
C 237.7
(3)
P 		189.8
(-2.2)
P 		190
(-2.2)
P 		182.8
(-3)
P 		212
(0.2)
P 		187.7
(-2.5)
P 		55
avr 200.2
(0.3) 		197.9
(0.2) 		150.2
(-3) 		216.8
(1.4) 		240.7
(3) 		192.4
(-0.2) 		91
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190100_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6187
Sequence Type Exemplar sequence
Representative Public ID R151.5 NCBI
Target Description R151.5 /REP_DB=WormBase Gene ID /WP=CE27200 /GEN=toh-2 /GB=AAK29859.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027560(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027560 NCBI Caenorhabditis elegans TOllisH (Tolloid and BMP-1 family) family member (toh-2) (toh-2) mRNA, complete cds. 10/11 None
SNAP00000037932 ENSEMBL cdna:SNAP chromosome:CEL140:III:7202586:7207353:1 9/11 None
GENEFINDER00000037943 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7202586:7207437:1 10/11 None
R151.5a ENSEMBL cdna:known chromosome:CEL140:III:7202585:7207555:1 gene:R151.5 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027561 5/11 Cross Hyb Matching Probes None
R151.5b 5/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176014 2 1 NM_001027560 190100_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7202583-7207842(+) 99.84 100.0
Public Domain and Genome References
Ensembl R151.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK29859 Tollish (tolloid and bmp-1 family) protein 2, isoform a [Caenorhabditis elegans] ref|NP_001022731.1| TOllisH (Tolloid and BMP-1 family) family member (toh-2) [Caenorhabditis elegans] sp|P98060|NAS35_CAEEL Zinc metalloproteinase nas-35 precursor (Nematode astacin 35) (Dumpy protein 31) (Tollish protein 2) 0.0
blast T16761 hypothetical protein R151.5 - Caenorhabditis elegans 0.0
blast CAE70154 Hypothetical protein CBG16621 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 6.6E-112
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 5.3E-9
Pfam IPR000859 EMBL-EBI CUB 1.3E-17
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 3.1E-13
Sequence
Target Sequence 
>C. ELEGANS:190100_AT
gacattctttgtgcatggactacgtagaagttagaaactctactgattttgcaaatactg
gaatgagatattgttgctatggaactcctccaactagaatccgttctgcaactactgata
tggttgtacttttccgaagcttctaccgtggtggtaaaggctttgaagcaagagcccgtg
cagtaccagaggcgggtaactggaattcctggagcccctggacggcatgttccgcaactt
gtggagcatgcggaagtcgcatgagaacccgaacatgtccacctggaaatgcatgctctg
gggagccagtagaaacacaaatttgcaatactcaagcttgtacgggtatgtgcgctcaga
aacgggaggaagagggtcaatgtggaggtttcctttctttgttacgaggcgtccgttgta
gacaagagaagacggtgatggctccatgcgagaacgcgtgctgtccaggtttcactcttc
agcgtggcagatgtgtccggtacctgatcaggtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACATTCTTTGTGCATGGACTACGT 405 371 1289 Antisense
GATATTGTTGCTATGGAACTCCTCC 21 427 1355 Antisense
GAACTCCTCCAACTAGAATCCGTTC 454 349 1370 Antisense
GAATCCGTTCTGCAACTACTGATAT 580 329 1385 Antisense
TATGGTTGTACTTTTCCGAAGCTTC 287 655 1407 Antisense
CAAGAGCCCGTGCAGTACCAGAGGC 248 185 1457 Antisense
GCGGGTAACTGGAATTCCTGGAGCC 453 291 1480 Antisense
GGCATGTTCCGCAACTTGTGGAGCA 16 537 1512 Antisense
CATGAGAACCCGAACATGTCCACCT 218 211 1548 Antisense
GTCCACCTGGAAATGCATGCTCTGG 365 477 1565 Antisense
ATGTGTCCGGTACCTGATCAGGTGC 171 49 1779 Antisense
Sequence Source Affymetrix Proprietary Database