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[190096_at] protease

Description F21F8.3 /REP_DB=WormBase Gene ID /WP=CE09539 /GEN=asp-5 /TR=O01532 /GB=AAC47991.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=protease
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 11      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 18      6grp_total > 32      4grp_total > 60      6grp_cgc > 60      6grp_high1_sub > De      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19662
1 2 3 4 range
A 20841.9
(2.5)
P 		22342
(3)
P 		18937.3
(1.8)
P 		5069.8
(-3)
P 		17272
B 22841.1
(3)
P 		18516.9
(1.7)
P 		19041.3
(1.8)
P 		3179.2
(-3)
P 		19662
C 18775.1
(2.8)
P 		19173.7
(3)
P 		17076.1
(2.2)
P 		4207.7
(-3)
P 		14966
avr 20819.4
(3) 		20010.9
(2.7) 		18351.6
(2.1) 		4152.2
(-3) 		16667

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15293
1 2 3 4 5 6 range
A 26528
(3)
P 		16950.8
(-1)
P 		20335.8
(0.4)
P 		17059
(-1)
P 		15485.1
(-1.7)
P 		12279.8
(-3)
P 		14248
B 18415.2
(3)
P 		17494.4
(2.1)
P 		15650.5
(0.3)
P 		17623
(2.2)
P 		13898.4
(-1.4)
P 		12301.5
(-3)
P 		6114
C 14770.3
(-0.4)
P 		17059.8
(1.3)
P 		19360.4
(3)
P 		18121.8
(2.1)
P 		15961.8
(0.5)
P 		11234.7
(-3)
P 		8126
avr 19904.5
(3) 		17168.3
(0.9) 		18448.9
(1.9) 		17601.3
(1.3) 		15115.1
(-0.6) 		11938.7
(-3) 		7966
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6911
1 2 3 4 range
A 7588.5
(3)
P 		4849.9
(0.6)
P 		4950.6
(0.7)
P 		742.1
(-3)
P 		6846
B 7283.8
(3)
P 		4573.3
(0.5)
P 		4412.3
(0.4)
P 		677.5
(-3)
P 		6606
C 6215.1
(3)
P 		5512.6
(2.2)
P 		5951.6
(2.7)
P 		709.5
(-3)
P 		5506
avr 7029.1
(3) 		4978.6
(1.1) 		5104.8
(1.2) 		709.7
(-3) 		6319

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3418
1 2 3 4 5 6 range
A 4890.2
(0.8)
P 		6028.5
(3)
P 		4428.7
(-0.2)
P 		4326.6
(-0.4)
P 		4672.7
(0.3)
P 		2989.7
(-3)
P 		3039
B 6407.6
(3)
P 		5823.2
(1.7)
P 		3800.4
(-3)
P 		4667.7
(-1)
P 		5646.8
(1.2)
P 		4587.2
(-1.2)
P 		2607
C 5474.7
(3)
P 		5205.5
(2.1)
P 		4927.5
(1.2)
P 		3644.3
(-3)
P 		5165
(2)
P 		3760.9
(-2.6)
P 		1830
avr 5590.8
(2.7) 		5685.7
(3) 		4385.5
(-1.1) 		4212.9
(-1.6) 		5161.5
(1.4) 		3779.3
(-3) 		1906
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190096_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12404
Sequence Type Exemplar sequence
Representative Public ID F21F8.3 NCBI
Target Description F21F8.3 /REP_DB=WormBase Gene ID /WP=CE09539 /GEN=asp-5 /TR=O01532 /GB=AAC47991.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072734(11), U34889(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072734 NCBI Caenorhabditis elegans F21F8.3 (protease) mRNA, complete cds. 11/11 A 
U34889 NCBI Caenorhabditis elegans aspartic protease (Ceasp1) mRNA, complete cds. 9/11 A 
SNAP00000008547 ENSEMBL cdna:SNAP chromosome:CEL140:V:8274977:8276578:1 11/11 None
GENEFINDER00000008563 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8274977:8278347:1 11/11 None
F21F8.3.2 ENSEMBL cdna:known chromosome:CEL140:V:8274973:8276637:1 gene:F21F8.3 11/11 None
F21F8.3.1 ENSEMBL cdna:known chromosome:CEL140:V:8274975:8276637:1 gene:F21F8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8274977-8276579(+) 100.0 100.0
Public Domain and Genome References
Gene Title protease
Gene Symbol asp-5
Ensembl F21F8.3
Entrez Gene 179211 Entrez gene
SwissProt O01532 EMBL-EBI
WormBase CE09539 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4190 aspartic-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4194 pepsin A activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC47991 Aspartyl protease protein 5 [Caenorhabditis elegans] ref|NP_505135.1| F21F8.3 [Caenorhabditis elegans] 0.0
blast CAE66086 Hypothetical protein CBG11303 [Caenorhabditis briggsae] 0.0
blast AAB06576 aspartic protease 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 5.8E-70
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 4.4E-75
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 1.7E-65
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 1.3E-7
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 4.2E-16
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505135.1 1 4-26
F21F8.3.2 1 4-26
F21F8.3.1 1 4-26
SNAP00000008547 1 4-26
GENEFINDER00000008563 1 5-22
Sequence
Target Sequence 
>C. ELEGANS:190096_AT
aacgctatcaaccaaggacttctcgatcaaccacttttcaccgtgtggcttgagcacaag
ggatctgccaacgatgttggaggaggtgtcttcacctacggagcagttgatactaccaac
tgcggaccagtgattgcctaccaaccactttcatctgccacatactatcaattcgtcgct
gattcattcaagcttggatcctattcaaactctaagaaataccaagttatctccgacact
ggaacttccttcctcggaggaccaaaggctgttgttgccgggctcgctagtgctcttgga
gctacttaccacagcaacgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGCTATCAACCAAGGACTTCTCG 473 145 613 Antisense
CAACCACTTTTCACCGTGTGGCTTG 114 187 640 Antisense
GCACAAGGGATCTGCCAACGATGTT 480 317 666 Antisense
ACTTTCATCTGCCACATACTATCAA 243 103 759 Antisense
GCCACATACTATCAATTCGTCGCTG 398 279 769 Antisense
TTCGTCGCTGATTCATTCAAGCTTG 116 695 784 Antisense
AAGCTTGGATCCTATTCAAACTCTA 466 151 802 Antisense
TTATCTCCGACACTGGAACTTCCTT 125 681 839 Antisense
ACCAAAGGCTGTTGTTGCCGGGCTC 472 83 873 Antisense
GGCTCGCTAGTGCTCTTGGAGCTAC 625 537 893 Antisense
TGGAGCTACTTACCACAGCAACGAC 560 545 909 Antisense
Sequence Source Affymetrix Proprietary Database