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[190094_s_at]

Description M02D8.4 /REP_DB=WormBase Gene ID /WP=CE04760 /TR=Q21463 /GB=AAA82381.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=asparagine synthase
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 57      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 58      6grp_total > 58      4grp_total > 16      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4278
1 2 3 4 range
A 1848.4
(-3)
P 		2195.4
(-2.5)
P 		3037.9
(-1.2)
P 		5909.2
(3)
P 		4061
B 1631.5
(-3)
P 		2189.8
(-1.9)
P 		3020.4
(-0.2)
P 		4640.6
(3)
P 		3009
C 1641.7
(-3)
P 		2141.9
(-1.9)
P 		2939.7
(-0.2)
P 		4389.2
(3)
P 		2748
avr 1707.2
(-3) 		2175.7
(-2.1) 		2999.3
(-0.6) 		4979.7
(3) 		3273

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3708
1 2 3 4 5 6 range
A 4021.9
(0.3)
P 		2873.8
(-1.7)
P 		2083.1
(-3)
P 		2678.1
(-2)
P 		2201.3
(-2.8)
P 		5616.7
(3)
P 		3534
B 3262.8
(0.6)
P 		2016
(-3)
P 		2276
(-2.3)
P 		2142.9
(-2.6)
P 		2379
(-2)
P 		4097.2
(3)
P 		2081
C 4731.8
(3)
P 		1908.5
(-3)
P 		3250.7
(-0.1)
P 		2140.2
(-2.5)
P 		3182.9
(-0.3)
P 		4643.1
(2.8)
P 		2823
avr 4005.5
(1.1) 		2266.1
(-3) 		2536.6
(-2.4) 		2320.4
(-2.9) 		2587.7
(-2.2) 		4785.7
(3) 		2520
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 548
1 2 3 4 range
A 673
(0)
P 		476.6
(-3)
P 		794.2
(1.9)
P 		865
(3)
P 		388
B 520.3
(-3)
P 		540.8
(-2.7)
P 		699.9
(-0.7)
P 		988.9
(3)
P 		469
C 543.5
(-3)
P 		615.8
(-2.1)
P 		1024.6
(3)
P 		740.1
(-0.5)
P 		481
avr 578.9
(-2.4) 		544.4
(-3) 		839.6
(2.5) 		864.7
(3) 		320

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1324
1 2 3 4 5 6 range
A 741.4
(-1.1)
P 		1022.1
(0.7)
P 		453.7
(-3)
P 		679.2
(-1.5)
P 		664.3
(-1.6)
P 		1367.5
(3)
P 		914
B 1135.3
(0.6)
P 		671
(-2.3)
P 		552.7
(-3)
P 		567.6
(-2.9)
P 		966.6
(-0.5)
P 		1527.8
(3)
P 		975
C 1753.9
(3)
P 		582.3
(-2.3)
P 		827.4
(-1.2)
P 		430.4
(-3)
P 		1029.9
(-0.3)
P 		1554.3
(2.1)
P 		1324
avr 1210.2
(1.2) 		758.5
(-1.7) 		611.3
(-2.7) 		559.1
(-3) 		886.9
(-0.9) 		1483.2
(3) 		924
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190094_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16660
Sequence Type Exemplar sequence
Representative Public ID M02D8.4 NCBI
Target Description M02D8.4 /REP_DB=WormBase Gene ID /WP=CE04760 /TR=Q21463 /GB=AAA82381.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=asparagine synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171746(11), NM_171747(11), NM_001029628(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171746 NCBI Caenorhabditis elegans M02D8.4a (M02D8.4) mRNA, complete cds. 11/11 None
NM_171747 NCBI Caenorhabditis elegans M02D8.4b (M02D8.4) mRNA, complete cds. 11/11 None
NM_001029628 NCBI Caenorhabditis elegans M02D8.4c (M02D8.4) mRNA, complete cds. 10/11 None
SNAP00000039680 ENSEMBL cdna:SNAP chromosome:CEL140:X:8748548:8750800:-1 11/11 None
GENEFINDER00000039685 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8748548:8750800:-1 11/11 None
M02D8.4a ENSEMBL cdna:known chromosome:CEL140:X:8748124:8750800:-1 gene:M02D8.4 11/11 None
M02D8.4b.1 ENSEMBL cdna:known chromosome:CEL140:X:8748369:8750907:-1 gene:M02D8.4 11/11 None
M02D8.4b.2 ENSEMBL cdna:known chromosome:CEL140:X:8748369:8750907:-1 gene:M02D8.4 11/11 None
M02D8.4b.3 ENSEMBL cdna:known chromosome:CEL140:X:8748401:8750879:-1 gene:M02D8.4 11/11 None
M02D8.4c ENSEMBL cdna:known chromosome:CEL140:X:8748639:8750800:-1 gene:M02D8.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8748546-8750799(-) 100.0 100.0
Public Domain and Genome References
Ensembl M02D8.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741864 M02D8.4a [Caenorhabditis elegans] gb|AAA82381.1| Hypothetical protein M02D8.4a [Caenorhabditis elegans] 0.0
blast NP_001024799 M02D8.4c [Caenorhabditis elegans] gb|AAU05557.1| Hypothetical protein M02D8.4c [Caenorhabditis elegans] 0.0
blast CAE69352 Hypothetical protein CBG15441 [Caenorhabditis briggsae] 0.0
blast NP_741865 M02D8.4b [Caenorhabditis elegans] gb|AAN72427.1| Hypothetical protein M02D8.4b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000583 EMBL-EBI Glutamine amidotransferase, class-II 3.2E-10
Pfam IPR001962 EMBL-EBI Asparagine synthase 1.0E-126
Pfam IPR001962 EMBL-EBI Asparagine synthase 1.3E-51
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741865.1 1 368-387
M02D8.4b.1 1 368-387
M02D8.4b.2 1 368-387
M02D8.4b.3 1 368-387
Sequence
Target Sequence 
>C. ELEGANS:190094_S_AT
aaaccatcgatcgtgttcttcatctctacacatctgattgtcttcgtgctgacaagtctt
cgatggctcactcagtcgaagttcgtgttccattcctggataaagcatttgttgaggctg
cagtcagtttggaccctgcatttaagcgcccacaaaaacttgaagatgggagaaactgcg
agaagtttgttctccgatcagctttcaacacggaccaatacccatatctgcctgatgaga
ttttgtggagacaaaaagaacagttctccgacggagttggatacagctggattgacactc
tcatgaagtactgtgctgctaaaattaccgacaaggaattctctcaagcctctaagcttt
tcccacacaatactccacacagtaaggaagcattctacatgagaaagatcttccacgagc
agttcccatctgagcaagcggctctcactgtcagaaaatggattccaaaatggcaaaaga
accaagatccatcaggaagagcatcattggtccatgagaactctgctcacaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACCATCGATCGTGTTCTTCATCT 27 127 1109 Antisense
CACATCTGATTGTCTTCGTGCTGAC 584 195 1137 Antisense
TGCTGACAAGTCTTCGATGGCTCAC 13 583 1155 Antisense
CAGTCGAAGTTCGTGTTCCATTCCT 612 207 1181 Antisense
AGTTTGGACCCTGCATTTAAGCGCC 196 59 1234 Antisense
AGAAGTTTGTTCTCCGATCAGCTTT 234 75 1289 Antisense
GATCAGCTTTCAACACGGACCAATA 150 421 1304 Antisense
ACCAATACCCATATCTGCCTGATGA 351 83 1322 Antisense
AGGAATTCTCTCAAGCCTCTAAGCT 643 53 1442 Antisense
TCTGAGCAAGCGGCTCTCACTGTCA 516 607 1537 Antisense
GGTCCATGAGAACTCTGCTCACAAC 593 501 1617 Antisense
Sequence Source Affymetrix Proprietary Database