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[190069_at]

Description ZK455.8 /REP_DB=WormBase Gene ID /WP=CE24726 /CHR=X /FEA=Sanger Annotation /DEF=membrane transporter (HINXTON) TR:Q23501 protein_id:CAA91492.2
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 85      4grp_highest_8_8 > 05      4grp_high_8_8 > 30      6grp_highest_nonorm > 81      6grp_total > 87      4grp_total > 24      6grp_highest_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 174.7
(-3)
P 		318.3
(2.3)
P 		271
(0.6)
P 		336.1
(3)
P 		161
B 191.8
(-3)
P 		228.2
(-1.2)
P 		281.4
(1.5)
P 		310
(3)
P 		118
C 221.3
(-3)
P 		299.4
(0.1)
P 		341.9
(1.9)
P 		370.1
(3)
P 		149
avr 195.9
(-3) 		282
(0.6) 		298.1
(1.3) 		338.7
(3) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 213
1 2 3 4 5 6 range
A 170.5
(-3)
P 		276.9
(1.7)
P 		205.1
(-1.5)
P 		279.1
(1.8)
P 		274.3
(1.6)
P 		306.9
(3)
P 		136
B 183.3
(-3)
P 		200.3
(-2.4)
P 		305.6
(1.1)
P 		221.3
(-1.7)
P 		304.2
(1.1)
P 		361.2
(3)
P 		178
C 160
(-3)
P 		194
(-2)
P 		326.6
(1.7)
P 		172.9
(-2.6)
P 		301.4
(1)
P 		373.2
(3)
P 		213
avr 171.3
(-3) 		223.7
(-1.2) 		279.1
(0.7) 		224.4
(-1.2) 		293.3
(1.2) 		347.1
(3) 		176
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 63.6
(1)
P 		69.1
(2.5)
P 		70.9
(3)
P 		49.2
(-3)
P 		22
B 61.2
(-0)
P 		56.4
(-3)
P 		65.2
(2.4)
P 		66.1
(3)
P 		10
C 73.2
(-1.9)
P 		86.1
(-0.5)
P 		119.2
(3)
P 		62.4
(-3)
P 		57
avr 66
(-1.4) 		70.5
(-0.4) 		85.1
(3) 		59.2
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 31.4
(-3)
P 		98.5
(3)
P 		44.7
(-1.8)
P 		70.8
(0.5)
P 		82.8
(1.6)
P 		74.7
(0.9)
P 		67
B 63.8
(-2.6)
P 		66.7
(-2.4)
P 		74.2
(-1.8)
P 		58.6
(-3)
P 		123.6
(2.1)
P 		134.7
(3)
P 		76
C 59.3
(-1.4)
P 		59.2
(-1.4)
P 		83.1
(0.2)
P 		34.8
(-3)
P 		97.5
(1.2)
P 		124.9
(3)
P 		90
avr 51.5
(-3) 		74.8
(-0.7) 		67.3
(-1.4) 		54.7
(-2.7) 		101.3
(2) 		111.4
(3) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190069_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16034
Sequence Type Exemplar sequence
Representative Public ID ZK455.8 NCBI
Target Description ZK455.8 /REP_DB=WormBase Gene ID /WP=CE24726 /CHR=X /FEA=Sanger Annotation /DEF=membrane transporter (HINXTON) TR:Q23501 protein_id:CAA91492.2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077498(11), NM_001029821(9), AF199013(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077498 NCBI Caenorhabditis elegans ZK455.8a (ZK455.8) mRNA, complete cds. 11/11 None
NM_001029821 NCBI Caenorhabditis elegans ZK455.8b (ZK455.8) mRNA, complete cds. 9/11 None
AF199013 NCBI Caenorhabditis elegans organic cation transporter (oct2) mRNA, complete cds. 9/11 None
ZK455.8b ENSEMBL cdna:known chromosome:CEL140:X:11346379:11349843:1 gene:ZK455.8 9/11 None
ZK455.8a ENSEMBL cdna:known chromosome:CEL140:X:11346380:11350155:1 gene:ZK455.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000004978 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11346379-11350058(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK455.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91492 Hypothetical protein ZK455.8a [Caenorhabditis elegans] ref|NP_509899.3| ZK455.8a [Caenorhabditis elegans] 0.0
blast CAC42386 Hypothetical protein ZK455.8b [Caenorhabditis elegans] ref|NP_001024992.1| ZK455.8b [Caenorhabditis elegans] 0.0
blast CAE70653 Hypothetical protein CBG17359 [Caenorhabditis briggsae] 0.0
blast AAG28557 organic cation transporter [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 2.4E-14
Pfam IPR005828 EMBL-EBI General substrate transporter 2.4E-14
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509899.3 11 310-329,336-355,360-382,394-416,420-442,511-530,545-567,576-594,599-621,634-656,661-683
NP_001024992.1 11 310-329,336-355,360-382,394-416,420-442,511-530,545-567,576-594,599-621,634-656,661-683
AAG28557.1 10 21-40,50-67,106-128,132-154,223-242,257-279,288-306,311-333,346-368,373-395
ZK455.8b 11 310-329,336-355,360-382,394-416,420-442,511-530,545-567,576-594,599-621,634-656,661-683
ZK455.8a 11 310-329,336-355,360-382,394-416,420-442,511-530,545-567,576-594,599-621,634-656,661-683
Sequence
Target Sequence 
>C. ELEGANS:190069_AT
agatcacagatgcttgttctcttcgggtccggtctattcctgttaacgtcagtagtaatg
gtatacagaaaacaatctactttggcactcattttcatgttactcagcaaagcttgtatt
caaggctcatttaatatattgtacatctttacgtctgaactcaatccgacggtcgtgaga
aacagtgcagtaggaatatcatctatggtcgctagaatgggagctggtgcatctggttat
attgcaattctttctgacgtcactatgcccttagtaccgatgacaatttttgcgtgtttc
tcattattggcggggtgtttggtactcttactgccggaaactcaaggattgccattgcca
gatactattttggactctgtacaaatggtcaaacgaaacaccaagccatgcggaactttg
tccgggacatttttaggaggcattgacgacgatgcacaaccgtacggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATCACAGATGCTTGTTCTCTTCG 465 65 1840 Antisense
TCCGGTCTATTCCTGTTAACGTCAG 633 593 1867 Antisense
ATCTACTTTGGCACTCATTTTCATG 2 33 1914 Antisense
ACATCTTTACGTCTGAACTCAATCC 312 105 1982 Antisense
ATTGCAATTCTTTCTGACGTCACTA 610 5 2080 Antisense
GTCACTATGCCCTTAGTACCGATGA 451 459 2098 Antisense
GCGTGTTTCTCATTATTGGCGGGGT 608 291 2131 Antisense
TTTGGTACTCTTACTGCCGGAAACT 284 665 2157 Antisense
GGATTGCCATTGCCAGATACTATTT 166 509 2185 Antisense
CACCAAGCCATGCGGAACTTTGTCC 405 203 2238 Antisense
GACGACGATGCACAACCGTACGGTG 503 363 2284 Antisense
Sequence Source Affymetrix Proprietary Database