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[190052_at] glycosyl hydrolase

Description F57B10.7 /REP_DB=WormBase Gene ID /WP=CE11310 /TR=O44736 /GB=AAB96724.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=glycosyl hydrolase
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 54      4grp_highest_8_8 > 38      4grp_high_8_8 > 04      6grp_highest_nonorm > 76      6grp_total > 55      4grp_total > 05      6grp_cgc > 46      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 480
1 2 3 4 range
A 736.9
(1.4)
P 		526.6
(-3)
P 		600.2
(-1.5)
P 		811.6
(3)
P 		285
B 662.3
(-1.6)
P 		556.9
(-3)
P 		595
(-2.5)
P 		1006.7
(3)
P 		450
C 680
(-0.7)
P 		553.5
(-3)
P 		624.5
(-1.7)
P 		887.6
(3)
P 		334
avr 693.1
(-0.5) 		545.7
(-3) 		606.6
(-2) 		902
(3) 		356

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 743
1 2 3 4 5 6 range
A 879.3
(-1.7)
P 		778.8
(-2.7)
P 		1343.8
(3)
P 		752.5
(-3)
P 		958.1
(-0.9)
P 		917.2
(-1.3)
P 		591
B 965.8
(-2.7)
P 		1158.8
(-0.5)
P 		1468.8
(3)
P 		1108.6
(-1.1)
P 		941.3
(-3)
P 		1322.9
(1.3)
P 		528
C 973.9
(0.2)
P 		1163.2
(2.6)
P 		845.7
(-1.5)
P 		921.8
(-0.5)
P 		726
(-3)
P 		1194.4
(3)
P 		468
avr 939.7
(-1.9) 		1033.6
(-0.2) 		1219.4
(3) 		927.6
(-2.1) 		875.1
(-3) 		1144.8
(1.7) 		344
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 268.3
(3)
P 		114.3
(-3)
P 		156.9
(-1.3)
P 		118.8
(-2.8)
P 		154
B 211.2
(2.7)
P 		137.5
(-3)
P 		137.9
(-3)
P 		214.5
(3)
P 		77
C 225.1
(3)
P 		159.1
(-2.3)
P 		217.7
(2.4)
P 		149.7
(-3)
P 		75
avr 234.9
(3) 		137
(-3) 		170.8
(-0.9) 		161
(-1.5) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 331
1 2 3 4 5 6 range
A 162.1
(-3)
P 		277
(2.3)
P 		292.7
(3)
P 		190.9
(-1.7)
P 		289.1
(2.8)
P 		223.3
(-0.2)
P 		131
B 336
(-1.7)
P 		385.7
(-0.2)
P 		356.7
(-1.1)
P 		293.6
(-3)
P 		382.5
(-0.3)
P 		493.3
(3)
P 		200
C 361
(1.9)
P 		354.9
(1.7)
P 		215.2
(-2.2)
P 		185.4
(-3)
P 		234.9
(-1.6)
P 		399.8
(3)
P 		214
avr 286.4
(-0.5) 		339.2
(1.7) 		288.2
(-0.4) 		223.3
(-3) 		302.2
(0.2) 		372.1
(3) 		149
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190052_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.687
Sequence Type Exemplar sequence
Representative Public ID F57B10.7 NCBI
Target Description F57B10.7 /REP_DB=WormBase Gene ID /WP=CE11310 /TR=O44736 /GB=AAB96724.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=glycosyl hydrolase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059489(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059489 NCBI Caenorhabditis elegans TREhalase family member (tre-1) (tre-1) mRNA, complete cds. 11/11 None
SNAP00000011830 ENSEMBL cdna:SNAP chromosome:CEL140:I:6563035:6570433:1 9/11 None
GENEFINDER00000011844 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6563035:6571669:1 11/11 None
F57B10.7 ENSEMBL cdna:known chromosome:CEL140:I:6562863:6566727:1 gene:F57B10.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6562963-6566394(+) 100.0 100.0
Public Domain and Genome References
Gene Title glycosyl hydrolase
Gene Symbol tre-1
Ensembl F57B10.7
Entrez Gene 172371 Entrez gene
SwissProt O44736 EMBL-EBI
WormBase CE30545 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:14663_S_AT glycosyl hydrolase family protein 37 / trehalase, putative at
ATH1-121501:254197_AT glycosyl hydrolase family protein 37 / trehalase, putative at
CANINE_2:CFAAFFX.19349.1.S1_AT similar to Trehalase precursor (Alpha,alpha-trehalase) (Alpha,alpha-trehalose glucohydrolase) cfa
DROSOPHILA_2:1635692_S_AT Trehalase dm
DROSGENOME1:152512_AT Trehalase dm
HG-U133_PLUS_2:207378_AT trehalase (brush-border membrane glycoprotein) hs
HG-U133A:207378_AT trehalase (brush-border membrane glycoprotein) hs
HG-FOCUS:207378_AT trehalase (brush-border membrane glycoprotein) hs
HG-U133A_2:207378_AT trehalase (brush-border membrane glycoprotein) hs
U133_X3P:G6005913_3P_AT trehalase (brush-border membrane glycoprotein) hs
HG-U95AV2:35529_AT trehalase (brush-border membrane glycoprotein) hs
MOUSE430A_2:1449430_A_AT trehalase (brush-border membrane glycoprotein) mm
MOUSE430_2:1449430_A_AT trehalase (brush-border membrane glycoprotein) mm
MOE430A:1449430_A_AT trehalase (brush-border membrane glycoprotein) mm
MG-U74BV2:163687_AT trehalase (brush-border membrane glycoprotein) mm
RG-U34A:AF038043_AT trehalase (brush-border membrane glycoprotein) rn
RAE230A:1371144_AT trehalase (brush-border membrane glycoprotein) rn
RAT230_2:1371144_AT trehalase (brush-border membrane glycoprotein) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5991 trehalose metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4555 alpha,alpha-trehalase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB96724 Trehalase protein 1 [Caenorhabditis elegans] ref|NP_491890.2| TREhalase family member (tre-1) [Caenorhabditis elegans] emb|CAD54510.1| trehalase [Caenorhabditis elegans] 0.0
blast CAE67221 Hypothetical protein CBG12658 [Caenorhabditis briggsae] 0.0
blast AAA97909 trehalase I 0.0
blast AAB96723 Hypothetical protein F57B10.6 [Caenorhabditis elegans] ref|NP_491891.1| F57B10.6 [Caenorhabditis elegans] 0.0
blast CAE67220 Hypothetical protein CBG12657 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006086 EMBL-EBI XPG I domain 6.5E-30
Pfam IPR006085 EMBL-EBI XPG N-terminal 1.4E-12
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 4.5E-48
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 9.6E-75
Pfam IPR006086 EMBL-EBI XPG I domain 6.5E-30
Pfam IPR006085 EMBL-EBI XPG N-terminal 4.4E-28
Pfam IPR000348 EMBL-EBI emp24/gp25L/p24 8.4E-37
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 2.1E-69
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 7.3E-72
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 9.6E-75
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000011844 1 1550-1572
Sequence
Target Sequence 
>C. ELEGANS:190052_AT
atcaaatgctgttcctttgtatgcaaaatgttatgatgatgatgatgatattccacatcg
tgttcatgattatttagagcgccaaggactattgaaatacacaaaaggtcttccaacatc
tcttgcaatgagtagtacgcagcaatgggataaggaaaacgcatggcctccaatgattca
tatggttatcgaaggattccgtacaactggtgacataaaactgatgaaagttgctgaaaa
gatggcaacgtcatggcttactggaacatatcagtcatttatcagaactcatgctatgtt
tgaaaagtataatgttactccacatactgaagaaacttctggaggaggtggaggagaata
tgaagttcaaactggctttggttggacaaatggcgttattttggatttattggacaaata
cggagatcaatttgcatccagctctacagcttccaaattcagtttttccctttcgaatat
cac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAAATGCTGTTCCTTTGTATGCA 439 27 1209 Antisense
GATGATATTCCACATCGTGTTCATG 36 411 1252 Antisense
CAAAAGGTCTTCCAACATCTCTTGC 226 191 1310 Antisense
AAACGCATGGCCTCCAATGATTCAT 187 127 1365 Antisense
GAAGGATTCCGTACAACTGGTGACA 96 335 1399 Antisense
GGCAACGTCATGGCTTACTGGAACA 567 533 1452 Antisense
GTCATTTATCAGAACTCATGCTATG 376 467 1482 Antisense
GTATAATGTTACTCCACATACTGAA 355 451 1515 Antisense
CAATTTGCATCCAGCTCTACAGCTT 73 181 1636 Antisense
AGCTCTACAGCTTCCAAATTCAGTT 469 79 1648 Antisense
TCAGTTTTTCCCTTTCGAATATCAC 334 623 1667 Antisense
Sequence Source Affymetrix Proprietary Database