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[190049_at] enoyl-coA hydratase

Description F43H9.1 /REP_DB=WormBase Gene ID /WP=CE07245 /TR=Q20376 /GB=AAA92304.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=enoyl-coA hydratase
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 72      6grp_total > 60      4grp_total > 04      6grp_highest_sub > 03_04      6grp_cgc > 94      6grp_high1_sub > 03_04      6grp_cgc_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 497
1 2 3 4 range
A 324.1
(-1.6)
P 		227.3
(-3)
P 		236.4
(-2.9)
P 		644.9
(3)
P 		418
B 335.6
(-2.2)
P 		287.3
(-3)
P 		292.3
(-2.9)
P 		638.2
(3)
P 		351
C 355.5
(-2.1)
P 		291.9
(-3)
P 		317.4
(-2.6)
P 		723.9
(3)
P 		432
avr 338.4
(-2) 		268.8
(-3) 		282
(-2.8) 		669
(3) 		400

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 302
1 2 3 4 5 6 range
A 370.1
(0)
P 		292
(-3)
P 		384.6
(0.6)
P 		447.5
(3)
P 		339.5
(-1.2)
P 		379.5
(0.4)
P 		156
B 400
(-2.1)
P 		385.2
(-3)
P 		434.5
(0)
P 		482.3
(3)
P 		396.1
(-2.3)
P 		414
(-1.2)
P 		97
C 365
(-1.1)
P 		417.8
(-0.1)
P 		467.8
(0.9)
P 		573.6
(3)
P 		271.3
(-3)
P 		473.9
(1)
P 		302
avr 378.4
(-1.4) 		365
(-1.9) 		429
(0.4) 		501.1
(3) 		335.6
(-3) 		422.5
(0.2) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 118
(3)
P 		49.3
(-3)
P 		61.8
(-1.9)
P 		94.4
(0.9)
P 		69
B 107
(0.5)
P 		71
(-2.7)
P 		67.7
(-3)
P 		136
(3)
P 		68
C 117.7
(2.3)
P 		83.9
(-3)
P 		110.6
(1.2)
P 		122.1
(3)
P 		38
avr 114.2
(2.6) 		68.1
(-3) 		80
(-1.6) 		117.5
(3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 68.2
(-3)
P 		103.9
(1.7)
P 		83.7
(-0.9)
P 		113.5
(3)
P 		102.4
(1.5)
P 		92.4
(0.2)
P 		45
B 139.2
(0.6)
P 		128.2
(-0.5)
P 		105.5
(-3)
P 		127.7
(-0.6)
P 		160.9
(3)
P 		154.4
(2.3)
P 		55
C 135.3
(1)
P 		127.5
(0.4)
P 		119.1
(-0.3)
P 		115.4
(-0.7)
P 		87.8
(-3)
P 		158.6
(3)
P 		71
avr 114.2
(-0.9) 		119.9
(0.2) 		102.8
(-3) 		118.9
(-0) 		117
(-0.4) 		135.1
(3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190049_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11493
Sequence Type Exemplar sequence
Representative Public ID F43H9.1 NCBI
Target Description F43H9.1 /REP_DB=WormBase Gene ID /WP=CE07245 /TR=Q20376 /GB=AAA92304.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=enoyl-coA hydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072665(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072665 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-3) (ech-3) mRNA, complete cds. 11/11 None
SNAP00000036917 ENSEMBL cdna:SNAP chromosome:CEL140:V:8024966:8025840:1 11/11 None
GENEFINDER00000036923 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8024921:8025840:1 11/11 None
F43H9.1 ENSEMBL cdna:known chromosome:CEL140:V:8024885:8025894:1 gene:F43H9.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8024966-8025841(+) 100.0 100.0
Public Domain and Genome References
Gene Title enoyl-coA hydratase
Gene Symbol ech-3
Ensembl F43H9.1
Entrez Gene 179180 Entrez gene
SwissProt Q20376 EMBL-EBI
WormBase CE07245 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1637954_AT dm
DROSGENOME1:141345_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA92304 Enoyl-coa hydratase protein 3 [Caenorhabditis elegans] ref|NP_505066.1| Enoyl-CoA Hydratase family member (ech-3) [Caenorhabditis elegans] 1.0E-144
blast CAE66156 Hypothetical protein CBG11387 [Caenorhabditis briggsae] 1.0E-127
blast AAH83204 Hypothetical protein LOC449822 [Danio rerio] ref|NP_001005995.1| hypothetical protein LOC449822 [Danio rerio] 1.0E-60
blast AAH83204 Hypothetical protein LOC449822 [Danio rerio] ref|NP_001005995.1| hypothetical protein LOC449822 [Danio rerio] 3.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 8.3E-38
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 8.3E-38
Sequence
Target Sequence 
>C. ELEGANS:190049_AT
atgaaaactgctgtcctatacggagaaggtggtactttttgcgctggatatgatctggag
tctgtttcaaaagctgaacatcaagaagtttcggaagatttttgtgacaagtacagatat
atgggaccatcaatcatgaaaatcaagaaaccgttgattgctgcaattgaaggatttgca
gttgcaggtggtctagaactgagtctgatggctgatttacgtgtttcttctccttctgcc
aagtttggtgtcttttgtagacgagttggtgttccattgattgatggcggaacagttcgg
ttaccacgtgttatcggattgggaagagcactggatatgatactgactggacgggaagtt
ggagcacaagaagctcttcaatggggacttgtcaatagaatctcggatgaaggaaaagct
gttgaggaggcagtgaaactcggaaaacttattgcttctcatccggaaatatgcatgctg
gctgatagagaatccacgtattactcgttaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAAAACTGCTGTCCTATACGGAG 59 45 160 Antisense
GGTACTTTTTGCGCTGGATATGATC 647 503 190 Antisense
GTCTGATGGCTGATTTACGTGTTTC 411 471 362 Antisense
CTTCTCCTTCTGCCAAGTTTGGTGT 22 221 386 Antisense
GATGGCGGAACAGTTCGGTTACCAC 243 405 442 Antisense
TCGGTTACCACGTGTTATCGGATTG 292 157 456 Antisense
GGATATGATACTGACTGGACGGGAA 294 509 492 Antisense
GAAGCTCTTCAATGGGGACTTGTCA 182 341 529 Antisense
GAAAACTTATTGCTTCTCATCCGGA 406 355 602 Antisense
CATCCGGAAATATGCATGCTGGCTG 471 211 619 Antisense
GAGAATCCACGTATTACTCGTTAGA 505 389 647 Antisense
Sequence Source Affymetrix Proprietary Database