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[190040_at] zinc metalloprotease

Description F54E4.4 /REP_DB=WormBase Gene ID /WP=CE11080 /TR=Q93787 /GB=CAB01918.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_high1_10_10 > 51      4grp_high_8_8 > 36      6grp_total > 30      4grp_total > 52      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 90.5
(1.2)
P 		112
(3)
M 		60.9
(-1.2)
P 		39.5
(-3)
A 		73
B 110
(2.9)
P 		91.7
(-0.3)
P 		110.4
(3)
A 		76.3
(-3)
A 		34
C 109.2
(-0.6)
P 		151.5
(3)
P 		107.8
(-0.7)
P 		80.1
(-3)
P 		71
avr 103.2
(1.3) 		118.4
(3) 		93
(0.1) 		65.3
(-3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 65.3
(-1.6)
A 		108.4
(3)
P 		98.1
(1.9)
M 		90.2
(1.1)
P 		65.8
(-1.5)
A 		52.2
(-3)
A 		56
B 77.5
(-1)
M 		91.1
(0.1)
P 		128.1
(3)
P 		67.3
(-1.8)
A 		79.3
(-0.8)
P 		51.5
(-3)
A 		77
C 80.7
(-1.6)
P 		116.4
(0.7)
P 		115.6
(0.7)
P 		150.5
(3)
P 		59.6
(-3)
P 		72.7
(-2.1)
P 		91
avr 74.5
(-1.3) 		105.3
(2.1) 		113.9
(3) 		102.7
(1.8) 		68.2
(-2) 		58.8
(-3) 		55
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 33
(3)
P 		24.3
(1.1)
M 		15.9
(-0.8)
P 		5.8
(-3)
A 		27
B 35.1
(3)
P 		22.7
(-1)
P 		25.6
(-0)
A 		16.3
(-3)
A 		19
C 36.1
(1.5)
P 		43.6
(3)
P 		37.6
(1.8)
P 		13.5
(-3)
P 		30
avr 34.7
(3) 		30.2
(1.8) 		26.4
(0.8) 		11.9
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 12
(-3)
A 		38.5
(3)
P 		21.4
(-0.9)
M 		22.9
(-0.5)
P 		19.9
(-1.2)
A 		12.7
(-2.8)
A 		27
B 27
(0.8)
M 		30.3
(2.2)
P 		31.1
(2.5)
P 		17.8
(-3)
A 		32.2
(3)
P 		19.2
(-2.4)
A 		14
C 29.9
(0.9)
P 		35.5
(3)
P 		29.4
(0.7)
P 		30.3
(1.1)
P 		19.3
(-3)
P 		24.3
(-1.1)
P 		16
avr 23
(-1.4) 		34.8
(3) 		27.3
(0.2) 		23.7
(-1.1) 		23.8
(-1.1) 		18.7
(-3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190040_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17657
Sequence Type Exemplar sequence
Representative Public ID F54E4.4 NCBI
Target Description F54E4.4 /REP_DB=WormBase Gene ID /WP=CE11080 /TR=Q93787 /GB=CAB01918.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078033(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078033 NCBI Caenorhabditis elegans F54E4.4 (F54E4.4) mRNA, complete cds. 11/11 None
GENEFINDER00000032273 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14640445:14644089:-1 10/11 None
SNAP00000032271 ENSEMBL cdna:SNAP chromosome:CEL140:X:14641310:14644384:-1 10/11 None
F54E4.4 ENSEMBL cdna:known chromosome:CEL140:X:14641310:14644323:-1 gene:F54E4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14641309-14644323(-) 85.58 100.0
Public Domain and Genome References
Gene Title zinc metalloprotease
Ensembl F54E4.4
Entrez Gene 186247 Entrez gene
SwissProt Q93787 EMBL-EBI
WormBase CE11080 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01918 Hypothetical protein F54E4.4 [Caenorhabditis elegans] ref|NP_510434.1| F54E4.4 [Caenorhabditis elegans] 0.0
blast CAB01918 Hypothetical protein F54E4.4 [Caenorhabditis elegans] ref|NP_510434.1| F54E4.4 [Caenorhabditis elegans] 1.0E-178
blast CAE63210 Hypothetical protein CBG07566 [Caenorhabditis briggsae] 1.0E-154
blast CAB01918 Hypothetical protein F54E4.4 [Caenorhabditis elegans] ref|NP_510434.1| F54E4.4 [Caenorhabditis elegans] 1.0E-129
blast CAE63210 Hypothetical protein CBG07566 [Caenorhabditis briggsae] 2.0E-90
blast CAE63210 Hypothetical protein CBG07566 [Caenorhabditis briggsae] 9.0E-81
blast CAE60419 Hypothetical protein CBG04025 [Caenorhabditis briggsae] 2.0E-20
blast XP_786540 PREDICTED: similar to meprin beta family member (XB397) [Strongylocentrotus purpuratus] 1.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.0E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.6E-9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.0E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 8.8E-9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.0E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.6E-9
Sequence
Target Sequence 
>C. ELEGANS:190040_AT
aaaatctcgttttgcacaagcggaaaatatgagtcgagtatctgacgatcgaactggagg
aattttcaaaatttctcgtgtaaatcagaacaaaaacgcagaaatggtacgaatcgtcaa
gagaaaattggattttatcggtacaaccacaacaacgccagcaccaattccaacgccaaa
agtctgcgaggataaacacagtctctgttgtttttgggcaacgacgggagaatgcagtag
aaacccattctggatgaagttgaactgtgcaaaaacctgcgggacgtgtgattgtgcatc
cacaactacgacagctgataaatgtaaagattattacaaggagtgcagcttctgggctgt
acttggagaatgccaaagcaatccgttttatatgagacccaattgtcaaaagtcttgtaa
atcgtgtggagagcaagttaacacagtttatgcgcccacaccaagaccaggttgcgacaa
cggccacaacttatgcaattactgggctcacattggcgaatgctcatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAATCTCGTTTTGCACAAGCGGAA 418 133 591 Antisense
TATCGGTACAACCACAACAACGCCA 201 655 726 Antisense
ACACAGTCTCTGTTGTTTTTGGGCA 424 111 786 Antisense
GCGGGACGTGTGATTGTGCATCCAC 287 293 869 Antisense
GGAGTGCAGCTTCTGGGCTGTACTT 231 517 930 Antisense
CAATCCGTTTTATATGAGACCCAAT 599 179 969 Antisense
AGTTAACACAGTTTATGCGCCCACA 8 63 1026 Antisense
CGCCCACACCAAGACCAGGTTGCGA 316 255 1043 Antisense
GCGACAACGGCCACAACTTATGCAA 406 287 1064 Antisense
ACTTATGCAATTACTGGGCTCACAT 463 101 1079 Antisense
GGGCTCACATTGGCGAATGCTCATC 60 493 1094 Antisense
Sequence Source Affymetrix Proprietary Database