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[190031_at] EF hand (2 domains)

Description F21A10.1 /REP_DB=WormBase Gene ID /WP=CE18623 /TR=Q9XXN7 /GB=CAA16507.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=EF hand (2 domains)
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 50      4grp_high_8_8 > 43      6grp_highest_nonorm > 80      6grp_total > 31      4grp_total > 52      6grp_highest_sub > 04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 248
1 2 3 4 range
A 225.5
(3)
P 		212.8
(2.4)
P 		133
(-1)
P 		87.9
(-3)
P 		138
B 171.7
(2.1)
P 		201.2
(3)
P 		176.1
(2.2)
P 		11.8
(-3)
A 		189
C 260.1
(3)
P 		206.2
(1)
P 		182.7
(0.2)
P 		95.7
(-3)
P 		164
avr 219.1
(3) 		206.7
(2.5) 		163.9
(0.8) 		65.1
(-3) 		154

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 287
1 2 3 4 5 6 range
A 229.5
(0.8)
P 		209.6
(0.3)
P 		336.6
(3)
P 		234.1
(0.9)
P 		173.1
(-0.4)
P 		50
(-3)
P 		287
B 228.2
(1.7)
P 		271.6
(3)
P 		255.8
(2.5)
P 		255.2
(2.5)
P 		165.1
(-0.2)
P 		72.2
(-3)
P 		199
C 162.3
(-0.2)
P 		285.4
(3)
P 		208.6
(1)
P 		286.3
(3)
P 		145.1
(-0.7)
P 		57.1
(-3)
P 		229
avr 206.7
(1.3) 		255.5
(2.7) 		267
(3) 		258.5
(2.8) 		161.1
(-0.1) 		59.8
(-3) 		207
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 82.1
(3)
P 		46.2
(-0.1)
P 		34.8
(-1.1)
P 		12.9
(-3)
P 		69
B 54.7
(3)
P 		49.7
(2.4)
P 		40.8
(1.4)
P 		2.5
(-3)
A 		52
C 86.1
(3)
P 		59.3
(0.7)
P 		63.7
(1.1)
P 		16.1
(-3)
P 		70
avr 74.3
(3) 		51.7
(0.9) 		46.4
(0.4) 		10.5
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 42.3
(-0.1)
P 		74.6
(3)
P 		73.3
(2.9)
P 		59.4
(1.5)
P 		52.2
(0.8)
P 		12.2
(-3)
P 		62
B 79.4
(2)
P 		90.4
(3)
P 		62.1
(0.3)
P 		67.6
(0.8)
P 		67.1
(0.8)
P 		26.9
(-3)
P 		64
C 60.1
(0.6)
P 		87.1
(3)
P 		53.1
(0)
P 		57.6
(0.4)
P 		47
(-0.5)
P 		19.1
(-3)
P 		68
avr 60.6
(0.8) 		84
(3) 		62.8
(1) 		61.5
(0.9) 		55.4
(0.3) 		19.4
(-3) 		65
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190031_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17292
Sequence Type Exemplar sequence
Representative Public ID F21A10.1 NCBI
Target Description F21A10.1 /REP_DB=WormBase Gene ID /WP=CE18623 /TR=Q9XXN7 /GB=CAA16507.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=EF hand (2 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077307(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077307 NCBI Caenorhabditis elegans F21A10.1 (F21A10.1) mRNA, complete cds. 11/11 None
SNAP00000008355 ENSEMBL cdna:SNAP chromosome:CEL140:X:10179018:10180040:1 11/11 None
F21A10.1 ENSEMBL cdna:known chromosome:CEL140:X:10178974:10180504:1 gene:F21A10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10178973-10180504(+) 100.0 100.0
Public Domain and Genome References
Gene Title EF hand (2 domains)
Ensembl F21A10.1
Entrez Gene 184757 Entrez gene
SwissProt Q9XXN7 EMBL-EBI
WormBase CE18623 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA16507 Hypothetical protein F21A10.1 [Caenorhabditis elegans] ref|NP_509708.1| F21A10.1 [Caenorhabditis elegans] 1.0E-104
blast CAE70536 Hypothetical protein CBG17172 [Caenorhabditis briggsae] 1.0E-94
blast CAA16507 Hypothetical protein F21A10.1 [Caenorhabditis elegans] ref|NP_509708.1| F21A10.1 [Caenorhabditis elegans] 2.0E-78
blast CAE70536 Hypothetical protein CBG17172 [Caenorhabditis briggsae] 4.0E-75
blast CAB56836 DREAM protein [Homo sapiens] 8.0E-31
blast CAB56836 DREAM protein [Homo sapiens] 9.0E-25
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.1E-5
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0016
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 1.1E-5
Sequence
Target Sequence 
>C. ELEGANS:190031_AT
agagccttcaaagaaacatcaccaaacgctgtcatcaacaaaaatattatgagagaaaaa
tttgcggaattgtttcctcatggtgatattgaacattattctgatctcctttttgaaacg
tttgacaatgatgggaatggaacaatcaatttccaggagtttgtgaaagctctctcaatt
ctctgtcgtggcacgttggacgaaaagttagactggctatacaagttgtatgatccgaaa
gaaaaaggagaagttacttgggaccgattattctatgtgatcacatctatggatgattta
atgggtaagcacgccagaccacatcacacaagagagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGCCTTCAAAGAAACATCACCAA 153 69 73 Antisense
CATCACCAAACGCTGTCATCAACAA 537 213 89 Antisense
GGAATTGTTTCCTCATGGTGATATT 510 533 138 Antisense
GAACATTATTCTGATCTCCTTTTTG 328 351 163 Antisense
GATCTCCTTTTTGAAACGTTTGACA 648 411 175 Antisense
GTTTGTGAAAGCTCTCTCAATTCTC 178 453 231 Antisense
TCAATTCTCTGTCGTGGCACGTTGG 58 635 247 Antisense
GGAGAAGTTACTTGGGACCGATTAT 73 519 319 Antisense
GGGACCGATTATTCTATGTGATCAC 336 495 332 Antisense
AATGGGTAAGCACGCCAGACCACAT 129 163 372 Antisense
GCCAGACCACATCACACAAGAGAGC 107 281 385 Antisense
Sequence Source Affymetrix Proprietary Database