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[190024_at] zinc metalloprotease

Description F20G2.4 /REP_DB=WormBase Gene ID /WP=CE09507 /TR=Q93542 /GB=CAB02084.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_total > 83      4grp_total > 18      6grp_cgc > 87      6grp_cgc_sub > 05      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 4.6
(-3)
A 		34.9
(-1.5)
A 		6.2
(-2.9)
A 		128.5
(3)
P 		124
B 45.4
(-1.3)
A 		91.9
(2.2)
A 		22
(-3)
A 		103.3
(3)
P 		81
C 25.1
(-3)
A 		96.7
(0.3)
A 		32.8
(-2.6)
A 		155.7
(3)
P 		131
avr 25
(-2.7) 		74.5
(-0) 		20.3
(-3) 		129.2
(3) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 5.3
(-3)
A 		7.2
(-2.8)
A 		73.1
(3)
A 		58.9
(1.7)
M 		67.2
(2.5)
A 		53
(1.2)
A 		68
B 29.6
(-2.8)
A 		40.5
(-1.4)
A 		27.7
(-3)
A 		60.5
(1.2)
A 		75.1
(3)
A 		30.9
(-2.6)
A 		47
C 34
(1.2)
A 		38.2
(2.1)
A 		14.2
(-3)
A 		24.4
(-0.8)
A 		42.4
(3)
A 		21.1
(-1.5)
A 		28
avr 23
(-3) 		28.6
(-2.1) 		38.3
(-0.6) 		47.9
(0.9) 		61.6
(3) 		35
(-1.1) 		39
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 1.7
(-3)
A 		7.6
(-0.9)
A 		1.6
(-3)
A 		18.8
(3)
P 		17
B 14.5
(0.2)
A 		22.7
(3)
A 		5.1
(-3)
A 		22
(2.8)
P 		18
C 8.3
(-3)
A 		27.8
(3)
A 		11.4
(-2)
A 		26.3
(2.5)
P 		20
avr 8.2
(-2.2) 		19.4
(1.9) 		6
(-3) 		22.4
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 1
(-3)
A 		2.6
(-2.5)
A 		15.9
(1.6)
A 		14.9
(1.3)
M 		20.3
(3)
A 		12.9
(0.7)
A 		19
B 10.3
(-2.1)
A 		13.5
(-1.3)
A 		6.7
(-3)
A 		16
(-0.7)
A 		30.5
(3)
A 		11.5
(-1.8)
A 		24
C 12.6
(2.3)
A 		11.7
(1.8)
A 		3.6
(-3)
A 		4.9
(-2.2)
A 		13.7
(3)
A 		7.1
(-0.9)
A 		10
avr 8
(-3) 		9.3
(-2.4) 		8.7
(-2.7) 		11.9
(-1.3) 		21.5
(3) 		10.5
(-1.9) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190024_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14147
Sequence Type Exemplar sequence
Representative Public ID F20G2.4 NCBI
Target Description F20G2.4 /REP_DB=WormBase Gene ID /WP=CE09507 /TR=Q93542 /GB=CAB02084.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074008(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074008 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-24) (nas-24) mRNA, complete cds. 10/11 None
SNAP00000006710 ENSEMBL cdna:SNAP chromosome:CEL140:V:13772259:13773869:1 10/11 None
GENEFINDER00000006719 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13772259:13773869:1 10/11 None
F20G2.4 ENSEMBL cdna:known chromosome:CEL140:V:13772259:13773869:1 gene:F20G2.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13772258-13773869(+) 100.0 100.0
Public Domain and Genome References
Gene Title zinc metalloprotease
Gene Symbol nas-24
Ensembl F20G2.4
Entrez Gene 184744 Entrez gene
SwissProt Q93542 EMBL-EBI
WormBase CE35860 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02084 Hypothetical protein F20G2.4 [Caenorhabditis elegans] ref|NP_506409.2| Nematode AStacin protease family member (nas-24) [Caenorhabditis elegans] sp|Q93542|NAS24_CAEEL Zinc metalloproteinase nas-24 precursor (Nematode astacin 24) 0.0
blast CAA98504 Hypothetical protein K03B8.5 [Caenorhabditis elegans] ref|NP_505893.1| Nematode AStacin protease family member (nas-19) [Caenorhabditis elegans] sp|Q21181|NAS19_CAEEL Zinc metalloproteinase nas-19 precursor (Nematode astacin 19) 1.0E-173
blast CAE69666 Hypothetical protein CBG15914 [Caenorhabditis briggsae] 8.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 1.2E-19
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 5.3E-8
Sequence
Target Sequence 
>C. ELEGANS:190024_AT
aatactacggatgtgggtgctccactcaatgtaattgcccgaaaggattctttggaaaac
tttgtaacgaaagacgtcaacaagattcatatgaactaaaggctacctacggtcgatggc
agacacagactatctcatttaactacaagccagaaccagtctctgatggtttttattcaa
cttttgtgtacattactggtgaagctaactcaacaattgagattacaatggaaggccttg
aaaatgtgatatgtacggctggatgtacttggaacggtgttgaaattaagtcaagagaag
attctagaattaccagcccagtgatgtgctgcaaggatgaaccactgtataagaaagtat
tcaaatcacttcacaacccaaccatcattgaattgtactcaaaagaaacggccccgtcta
ctgctacattcaagtat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATACTACGGATGTGGGTGCTCCAC 94 163 644 Antisense
GGGTGCTCCACTCAATGTAATTGCC 500 497 658 Antisense
TAAAGGCTACCTACGGTCGATGGCA 327 637 740 Antisense
GTCGATGGCAGACACAGACTATCTC 353 473 755 Antisense
GACTATCTCATTTAACTACAAGCCA 311 373 771 Antisense
CAAGCCAGAACCAGTCTCTGATGGT 531 183 789 Antisense
TACGGCTGGATGTACTTGGAACGGT 656 647 897 Antisense
GATTCTAGAATTACCAGCCCAGTGA 262 431 943 Antisense
AGCCCAGTGATGTGCTGCAAGGATG 167 83 958 Antisense
TCAAAAGAAACGGCCCCGTCTACTG 535 625 1042 Antisense
CCCGTCTACTGCTACATTCAAGTAT 643 259 1056 Antisense
Sequence Source Affymetrix Proprietary Database