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[190011_at] polyketide synthase

Description C41A3.1 /REP_DB=WormBase Gene ID /WP=CE04187 /TR=Q18559 /GB=AAK18894.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=polyketide synthase
Cluster 6grp_high1_10_10 > 34      6grp_total > 02      4grp_total > 28      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 91.5
(1.1)
A 		108.6
(3)
A 		55.5
(-3)
A 		105.6
(2.7)
A 		53
B 116.4
(2.1)
A 		95.8
(0.1)
A 		64.9
(-3)
A 		125.5
(3)
P 		61
C 87.5
(-0.8)
A 		76.4
(-2)
A 		67.6
(-3)
A 		121.3
(3)
P 		54
avr 98.5
(0.9) 		93.6
(0.4) 		62.7
(-3) 		117.5
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 109.9
(0.5)
A 		134.8
(3)
P 		74.2
(-3)
A 		96.3
(-0.8)
M 		91
(-1.3)
M 		88.7
(-1.6)
P 		61
B 96.3
(-0.2)
P 		75.4
(-3)
M 		93.7
(-0.6)
P 		120.9
(3)
P 		90.8
(-1)
A 		78.7
(-2.6)
P 		46
C 86.4
(-2.9)
A 		123.1
(3)
P 		105.5
(0.2)
A 		89.5
(-2.4)
P 		85.6
(-3)
P 		101.1
(-0.5)
P 		38
avr 97.5
(-0.7) 		111.1
(3) 		91.1
(-2.5) 		102.2
(0.6) 		89.1
(-3) 		89.5
(-2.9) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 33.3
(3)
A 		23.6
(-0.1)
A 		14.5
(-3)
A 		15.5
(-2.7)
A 		19
B 37.1
(3)
A 		23.7
(-0.6)
A 		15
(-3)
A 		26.7
(0.2)
P 		22
C 29
(3)
A 		22
(-1.9)
A 		23.6
(-0.8)
A 		20.5
(-3)
P 		9
avr 33.1
(3) 		23.1
(-0.9) 		17.7
(-3) 		20.9
(-1.8) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 20.3
(-2.2)
A 		47.9
(3)
P 		16.2
(-3)
A 		24.4
(-1.4)
M 		27.5
(-0.9)
M 		21.6
(-2)
P 		32
B 33.5
(1.6)
P 		25.1
(-2)
M 		22.8
(-3)
P 		32
(0.9)
P 		36.9
(3)
A 		29.4
(-0.2)
P 		14
C 32
(1.3)
A 		37.6
(3)
P 		26.9
(-0.3)
A 		18
(-3)
P 		27.7
(-0)
P 		33.8
(1.8)
P 		20
avr 28.6
(-0.3) 		36.9
(3) 		22
(-3) 		24.8
(-1.9) 		30.7
(0.5) 		28.3
(-0.5) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190011_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16729
Sequence Type Exemplar sequence
Representative Public ID C41A3.1 NCBI
Target Description C41A3.1 /REP_DB=WormBase Gene ID /WP=CE04187 /TR=Q18559 /GB=AAK18894.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=polyketide synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076522(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076522 NCBI Caenorhabditis elegans C41A3.1 (C41A3.1) mRNA, complete cds. 11/11 None
SNAP00000015314 ENSEMBL cdna:SNAP chromosome:CEL140:X:5413775:5441883:1 11/11 None
GENEFINDER00000015548 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5413775:5441883:1 11/11 None
C41A3.1 ENSEMBL cdna:known chromosome:CEL140:X:5413775:5441883:1 gene:C41A3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5413775-5441884(+) 100.0 100.0
Public Domain and Genome References
Gene Title polyketide synthase
Ensembl C41A3.1
Entrez Gene 180815 Entrez gene
SwissProt Q18559 EMBL-EBI
WormBase CE04187 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6633 fatty acid biosynthesis inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
16740 transferase activity inferred from electronic annotation QuickGO AmiGO
48037 cofactor binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK18894 Hypothetical protein C41A3.1 [Caenorhabditis elegans] ref|NP_508923.1| C41A3.1 [Caenorhabditis elegans] 0.0
blast AAA98699 Similar to polyketide synthase. [Caenorhabditis briggsae] 0.0
blast CAE68751 Hypothetical protein CBG14683 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 6.0E-5
Protein Domains
Database ID Description E-Value
Pfam IPR001227 EMBL-EBI Acyl transferase domain 6.4E-30
Pfam IPR001227 EMBL-EBI Acyl transferase domain 3.6E-6
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 7.9E-57
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 4.4E-31
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 7.3E-43
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.2E-39
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.3E-52
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 0.0042
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 7.9E-34
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 9.9E-7
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 8.0E-4
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 6.2E-7
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.0E-8
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 4.3E-8
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 3.6E-4
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.5E-15
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.6E-5
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.7E-57
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.7E-38
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 5.2E-75
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.2E-48
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.7E-54
Pfam IPR001227 EMBL-EBI Acyl transferase domain 5.8E-24
Pfam IPR001227 EMBL-EBI Acyl transferase domain 3.6E-6
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 8.4E-21
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 7.3E-43
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.2E-39
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.3E-52
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 0.0042
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 7.9E-34
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 8.0E-4
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 6.2E-7
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.0E-8
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 4.3E-8
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 3.6E-4
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.5E-15
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.6E-5
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 5.2E-75
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.2E-48
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.7E-54
Pfam IPR001227 EMBL-EBI Acyl transferase domain 6.4E-30
Pfam IPR001227 EMBL-EBI Acyl transferase domain 3.6E-6
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 2.4E-41
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.7E-25
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 7.3E-43
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.2E-39
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.3E-52
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 0.0042
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 7.9E-34
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 9.9E-7
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 8.0E-4
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 6.2E-7
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.0E-8
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 4.3E-8
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 3.6E-4
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.5E-15
Pfam IPR006163 EMBL-EBI Phosphopantetheine-binding domain 1.6E-5
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.7E-38
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 5.2E-75
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.2E-48
Pfam IPR000794 EMBL-EBI Beta-ketoacyl synthase 1.7E-54
Sequence
Target Sequence 
>C. ELEGANS:190011_AT
aatacccgtcgaatgacctgcgtgaattggctcatttttatgctgaagagattgccgcac
atgccggaaacaagagaatcttcgttatggggcactcgatgggtggaataatgagtcgcg
aaatagtggctgagctcaaaatttggggatatgacattcccttcgtaatgctcttcgact
cttgggtgctcagaacaaacgaactcgacattgagaacataaagcaattcatcacatacg
tcttctctggactaccggatagtgagcatcgtattaatagagcgatcaaactagcccagc
ttcttcgagaatacaaaacctcagtctccgacacaaaattatatttattcaaaagcaaac
agcttggggacgccgcgttcaagaaagctgttcgctctgatttgaacgaagaactttctc
gatccatgacatgcaatggatttgatgaactgtctctgcagccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATACCCGTCGAATGACCTGCGTGA 343 173 22925 Antisense
GAGATTGCCGCACATGCCGGAAACA 214 397 22972 Antisense
GAATCTTCGTTATGGGGCACTCGAT 626 327 23000 Antisense
ATGACATTCCCTTCGTAATGCTCTT 479 45 23075 Antisense
TTCGACTCTTGGGTGCTCAGAACAA 289 695 23098 Antisense
AGCAATTCATCACATACGTCTTCTC 143 77 23147 Antisense
CGTCTTCTCTGGACTACCGGATAGT 501 245 23163 Antisense
CGATCAAACTAGCCCAGCTTCTTCG 202 249 23207 Antisense
GAATACAAAACCTCAGTCTCCGACA 356 189 23233 Antisense
GGACGCCGCGTTCAAGAAAGCTGTT 651 521 23292 Antisense
TTTGATGAACTGTCTCTGCAGCCAG 683 667 23365 Antisense
Sequence Source Affymetrix Proprietary Database