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[190007_at] Rhodanese-like domain

Description R186.6 /REP_DB=WormBase Gene ID /WP=CE20030 /TR=Q9N629 /GB=CAB76723.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Rhodanese-like domain
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 61      6grp_total > 51      4grp_total > 58      6grp_highest_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 630
1 2 3 4 range
A 195.7
(-1.5)
P 		615.5
(3)
P 		535
(2.1)
P 		51.3
(-3)
A 		564
B 226.2
(-0.8)
P 		587.2
(3)
P 		585.3
(3)
P 		11.5
(-3)
A 		576
C 216.8
(-1.4)
P 		459
(1.1)
P 		641.8
(3)
P 		65.6
(-3)
P 		576
avr 212.9
(-1.1) 		553.9
(2.6) 		587.4
(3) 		42.8
(-3) 		545

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 578
1 2 3 4 5 6 range
A 391.8
(0.6)
P 		408.1
(0.8)
P 		527.1
(2)
P 		624.1
(3)
P 		274.5
(-0.6)
P 		46
(-3)
P 		578
B 467.3
(2)
P 		356
(0.5)
P 		537.7
(3)
P 		474.4
(2.1)
P 		364.2
(0.6)
P 		96.3
(-3)
P 		441
C 577.9
(3)
P 		273.2
(-0.9)
P 		510.2
(2.1)
P 		467.2
(1.6)
P 		366.8
(0.3)
P 		112.8
(-3)
P 		465
avr 479
(2.4) 		345.8
(0.6) 		525
(3) 		521.9
(3) 		335.2
(0.4) 		85
(-3) 		440
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 221
1 2 3 4 range
A 71.2
(-0.1)
P 		133.6
(2.7)
P 		139.9
(3)
P 		7.5
(-3)
A 		132
B 72.1
(-0.1)
P 		145
(3)
P 		135.6
(2.6)
P 		2.4
(-3)
A 		143
C 71.8
(-1.3)
P 		132
(0.4)
P 		223.7
(3)
P 		11.1
(-3)
P 		213
avr 71.7
(-0.6) 		136.9
(1.9) 		166.4
(3) 		7
(-3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 72.2
(-0.5)
P 		145.2
(2.5)
P 		114.8
(1.2)
P 		158.3
(3)
P 		82.8
(-0.1)
P 		11.2
(-3)
P 		147
B 162.6
(3)
P 		118.5
(0.9)
P 		130.6
(1.5)
P 		125.7
(1.3)
P 		148
(2.3)
P 		35.9
(-3)
P 		127
C 214.2
(3)
P 		83.4
(-1.4)
P 		129.9
(0.1)
P 		93.9
(-1.1)
P 		118.7
(-0.2)
P 		37.7
(-3)
P 		177
avr 149.7
(3) 		115.7
(1.3) 		125.1
(1.8) 		126
(1.8) 		116.5
(1.4) 		28.3
(-3) 		121
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190007_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13526
Sequence Type Exemplar sequence
Representative Public ID R186.6 NCBI
Target Description R186.6 /REP_DB=WormBase Gene ID /WP=CE20030 /TR=Q9N629 /GB=CAB76723.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Rhodanese-like domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073841(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073841 NCBI Caenorhabditis elegans R186.6 (R186.6) mRNA, complete cds. 11/11 None
SNAP00000011785 ENSEMBL cdna:SNAP chromosome:CEL140:V:12961513:12963719:-1 9/11 None
GENEFINDER00000011798 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12961513:12963719:-1 9/11 None
R186.6 ENSEMBL cdna:known chromosome:CEL140:V:12961239:12961997:-1 gene:R186.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12961238-12961997(-) 95.13 100.0
Public Domain and Genome References
Gene Title Rhodanese-like domain
Ensembl R186.6
Entrez Gene 187919 Entrez gene
SwissProt Q9N629 EMBL-EBI
WormBase CE20030 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB76723 Hypothetical protein R186.6 [Caenorhabditis elegans] emb|CAB76412.1| Hypothetical protein R186.6 [Caenorhabditis elegans] ref|NP_506242.1| R186.6 [Caenorhabditis elegans] 1.0E-146
blast CAB76723 Hypothetical protein R186.6 [Caenorhabditis elegans] emb|CAB76412.1| Hypothetical protein R186.6 [Caenorhabditis elegans] ref|NP_506242.1| R186.6 [Caenorhabditis elegans] 3.0E-90
blast CAB76723 Hypothetical protein R186.6 [Caenorhabditis elegans] emb|CAB76412.1| Hypothetical protein R186.6 [Caenorhabditis elegans] ref|NP_506242.1| R186.6 [Caenorhabditis elegans] 4.0E-90
blast AAH74647 Thiosulfate sulfurtransferase (rhodanese) [Xenopus tropicalis] ref|NP_001005646.1| thiosulfate sulfurtransferase (rhodanese) [Xenopus tropicalis] 5.0E-18
blast AAH76754 Tst-prov protein [Xenopus laevis] 9.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR001763 EMBL-EBI Rhodanese-like 7.7E-14
Pfam IPR001763 EMBL-EBI Rhodanese-like 7.7E-14
Pfam IPR001763 EMBL-EBI Rhodanese-like 7.7E-14
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000011798 1 91-113
Sequence
Target Sequence 
>C. ELEGANS:190007_AT
gacgcattctctgttgatcatattgaatcagcccgcctcattttccattcaaatcttagt
catgctggcgttccagtacatccacttcaatttcaacgattcgcaagatctcagggaatt
gataatgattgtcacgtcatagtatatgatagaggacaaatgatttggagttcttatact
gtttggattttcaaattattcggacatcaaaaagtttcgctgctttcgggtggatatttg
ggatggaaaactcatcaagcaagaagtggacaatataaaacggagcaaggagatgctcca
agaaaaccaagacaaggagattttcttgcttcatggaatgattctgtaattataacgtat
gatgatgttcttctaaattctgaaattgacaattttgatgtagtggatgcacagacaaaa
gatgaattcttgggaactgctcaaggagcattgtatggacatataaaaggagcacggaat
attccagtggatgcagtatacgattgggctgttgggcagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGCATTCTCTGTTGATCATATTG 178 381 142 Antisense
TGATCATATTGAATCAGCCCGCCTC 607 565 156 Antisense
CAAATCTTAGTCATGCTGGCGTTCC 404 193 191 Antisense
TCATGCTGGCGTTCCAGTACATCCA 278 621 201 Antisense
AGTACATCCACTTCAATTTCAACGA 5 63 216 Antisense
TCAACGATTCGCAAGATCTCAGGGA 474 631 234 Antisense
AGTTTCGCTGCTTTCGGGTGGATAT 466 63 354 Antisense
GGAGATGCTCCAAGAAAACCAAGAC 95 519 430 Antisense
AAGGAGATTTTCTTGCTTCATGGAA 305 161 455 Antisense
AGATGAATTCTTGGGAACTGCTCAA 55 67 561 Antisense
GTATACGATTGGGCTGTTGGGCAGT 414 443 637 Antisense
Sequence Source Affymetrix Proprietary Database