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[190003_at]

Description F44C8.9 /REP_DB=WormBase Gene ID /WP=CE10394 /TR=O16355 /GB=AAB65896.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=zinc finger protein
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 12      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 90      6grp_total > 23      4grp_total > 15      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 191.2
(3)
P 		108.7
(-3)
P 		115.7
(-2.5)
P 		170.8
(1.5)
P 		83
B 171.5
(2.9)
P 		112
(-3)
P 		140.1
(-0.2)
P 		172.5
(3)
P 		61
C 188.2
(2.8)
P 		127.8
(-3)
P 		149.6
(-0.9)
P 		190.4
(3)
P 		63
avr 183.6
(3) 		116.2
(-3) 		135.1
(-1.3) 		177.9
(2.5) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 134.5
(-0.5)
P 		149
(0.5)
P 		186.9
(3)
P 		136.6
(-0.3)
P 		96.3
(-3)
P 		99
(-2.8)
P 		91
B 182.1
(3)
P 		169
(2)
P 		147.8
(0.3)
P 		141.1
(-0.3)
P 		116.2
(-2.3)
P 		107.1
(-3)
P 		75
C 212.2
(3)
P 		208.2
(2.8)
P 		177
(1.1)
P 		144.4
(-0.7)
P 		118.3
(-2.1)
P 		102.3
(-3)
P 		110
avr 176.3
(3) 		175.4
(2.9) 		170.6
(2.5) 		140.7
(0.1) 		110.3
(-2.4) 		102.8
(-3) 		74
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 69.6
(3)
P 		23.6
(-3)
P 		30.2
(-2.1)
P 		25
(-2.8)
P 		46
B 54.7
(3)
P 		27.7
(-3)
P 		32.5
(-1.9)
P 		36.8
(-1)
P 		27
C 62.3
(3)
P 		36.7
(-2.1)
P 		52.1
(1)
P 		32.1
(-3)
P 		30
avr 62.2
(3) 		29.3
(-3) 		38.3
(-1.4) 		31.3
(-2.6) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 24.8
(-2.9)
P 		53
(3)
P 		40.7
(0.4)
P 		34.6
(-0.8)
P 		29.1
(-2)
P 		24.1
(-3)
P 		29
B 63.4
(3)
P 		56.3
(1.5)
P 		35.9
(-3)
P 		37.4
(-2.7)
P 		47.2
(-0.5)
P 		39.9
(-2.1)
P 		28
C 78.7
(3)
P 		63.5
(1.2)
P 		45.1
(-1.1)
P 		29
(-3)
P 		38.3
(-1.9)
P 		34.3
(-2.4)
P 		50
avr 55.6
(2.5) 		57.6
(3) 		40.6
(-1.1) 		33.7
(-2.8) 		38.2
(-1.7) 		32.8
(-3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190003_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15161
Sequence Type Exemplar sequence
Representative Public ID F44C8.9 NCBI
Target Description F44C8.9 /REP_DB=WormBase Gene ID /WP=CE10394 /TR=O16355 /GB=AAB65896.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=zinc finger protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028755(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028755 NCBI Caenorhabditis elegans F44C8.9a (F44C8.9) mRNA, complete cds. 11/11 None
SNAP00000011140 ENSEMBL cdna:SNAP chromosome:CEL140:V:2212761:2227130:-1 11/11 None
GENEFINDER00000011146 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2212761:2214288:-1 11/11 None
F44C8.9a ENSEMBL cdna:known chromosome:CEL140:V:2212761:2214288:-1 gene:F44C8.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
185730 2 1 NM_001028755 190003_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2212760-2214288(-) 100.0 100.0
Public Domain and Genome References
Ensembl F44C8.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAQ62455 Hypothetical protein F44C8.9a [Caenorhabditis elegans] ref|NP_001023926.1| F44C8.9a [Caenorhabditis elegans] 0.0
blast T31787 hypothetical protein F44C8.9 - Caenorhabditis elegans 0.0
blast AAQ62455 Hypothetical protein F44C8.9a [Caenorhabditis elegans] ref|NP_001023926.1| F44C8.9a [Caenorhabditis elegans] 1.0E-149
blast T31787 hypothetical protein F44C8.9 - Caenorhabditis elegans 1.0E-141
blast CAE58160 Hypothetical protein CBG01250 [Caenorhabditis briggsae] 1.0E-139
Protein Domains
Database ID Description E-Value
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 1.8E-8
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 1.2E-5
Sequence
Target Sequence 
>C. ELEGANS:190003_AT
ttggcgaccgcgaactggtacgcaagacttccagattttacaagtctggatcttcgtgtt
aagctggaaatactaaaatccacgtggatgctttggattcgactgaacaagctagctgaa
acagcacaaaatcaaagaattaaagaaatgggtgcaaacatttttatgtgctcagaaaac
acttgtctgaatgtgcaggatatcaatgttgactggagctggtgtaccaattacggtgca
gagcaaatgcaagcattcctaatgccaaatattgataagcattggagacagccagtggaa
gacctcgtgaagctcaacccaacagatatggagctcaacttcatgctcattcaactctgc
cttaacgatgctggcaagaaatttcaaggacgtgtcctagaggcaactgataagctcttg
caaatcaatgcagataatttgcataattattataccaaaaagttgaacatgccatcatac
tctggacgattgacaaaactgatgaaaatcaaccaaattgtcgaggaagatgtgagggag
agaaaggaaaaagcgaagattgtcgaagtttttgacctgttttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGCGACCGCGAACTGGTACGCAA 173 709 655 Antisense
GGTACGCAAGACTTCCAGATTTTAC 301 503 671 Antisense
TCTGGATCTTCGTGTTAAGCTGGAA 402 607 699 Antisense
GATGCTTTGGATTCGACTGAACAAG 477 413 741 Antisense
GCTGGTGTACCAATTACGGTGCAGA 278 295 872 Antisense
GCCAGTGGAAGACCTCGTGAAGCTC 110 281 945 Antisense
CGTGAAGCTCAACCCAACAGATATG 586 243 960 Antisense
TTCAACTCTGCCTTAACGATGCTGG 362 685 1004 Antisense
AGGACGTGTCCTAGAGGCAACTGAT 370 55 1041 Antisense
GAACATGCCATCATACTCTGGACGA 72 351 1119 Antisense
TGTCGAAGTTTTTGACCTGTTTTCA 282 557 1215 Antisense
Sequence Source Affymetrix Proprietary Database