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[190002_at] glucosylceramidase

Description C33C12.8 /REP_DB=WormBase Gene ID /WP=CE08556 /TR=O16581 /GB=AAB66060.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=glucosylceramidase
Cluster 6grp_total > 08      4grp_total > 03      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 445
1 2 3 4 range
A 47.7
(-2.4)
A 		11.8
(-2.9)
A 		4.5
(-3)
A 		448.2
(3)
P 		444
B 44.3
(-2.3)
A 		12.1
(-2.9)
A 		4.3
(-3)
A 		370.1
(3)
P 		366
C 49.4
(-2.1)
A 		5.8
(-3)
A 		3.6
(-3)
A 		314.7
(3)
P 		311
avr 47.1
(-2.3) 		9.9
(-2.9) 		4.1
(-3) 		377.7
(3) 		374

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 45
(3)
A 		4.3
(-2.7)
A 		5.9
(-2.5)
A 		2.1
(-3)
A 		3.4
(-2.8)
A 		15.1
(-1.2)
A 		43
B 40.5
(3)
A 		4.9
(-2.6)
A 		2.5
(-3)
A 		4.6
(-2.6)
A 		2.2
(-3)
A 		3.2
(-2.8)
A 		38
C 49.1
(3)
A 		5.7
(-2.6)
A 		5.3
(-2.7)
A 		4.3
(-2.8)
A 		2.9
(-3)
A 		2.6
(-3)
A 		47
avr 44.9
(3) 		5
(-2.7) 		4.6
(-2.7) 		3.7
(-2.9) 		2.8
(-3) 		7
(-2.4) 		42
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 17.4
(-1.5)
A 		2.6
(-2.9)
A 		1.2
(-3)
A 		65.6
(3)
P 		64
B 14.1
(-2)
A 		3
(-2.8)
A 		1
(-3)
A 		78.9
(3)
P 		78
C 16.3
(-1.3)
A 		1.7
(-2.9)
A 		1.2
(-3)
A 		53.1
(3)
P 		52
avr 15.9
(-1.6) 		2.4
(-2.9) 		1.1
(-3) 		65.9
(3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 8.3
(3)
A 		1.5
(-2.2)
A 		1.3
(-2.4)
A 		0.5
(-3)
A 		1
(-2.6)
A 		3.7
(-0.5)
A 		8
B 14.1
(3)
A 		1.6
(-2.6)
A 		0.6
(-3)
A 		1.2
(-2.7)
A 		0.9
(-2.9)
A 		1.2
(-2.7)
A 		14
C 18.2
(3)
A 		1.7
(-2.7)
A 		1.3
(-2.9)
A 		0.9
(-3)
A 		0.9
(-3)
A 		0.9
(-3)
A 		17
avr 13.5
(3) 		1.6
(-2.7) 		1.1
(-2.9) 		0.9
(-3) 		0.9
(-3) 		1.9
(-2.5) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190002_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4295
Sequence Type Exemplar sequence
Representative Public ID C33C12.8 NCBI
Target Description C33C12.8 /REP_DB=WormBase Gene ID /WP=CE08556 /TR=O16581 /GB=AAB66060.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=glucosylceramidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061807(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061807 NCBI Caenorhabditis elegans C33C12.8 (glucosylceramidase) mRNA, complete cds. 11/11 None
SNAP00000027205 ENSEMBL cdna:SNAP chromosome:CEL140:II:2170897:2194822:-1 11/11 None
GENEFINDER00000027216 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2182462:2186422:-1 11/11 None
C33C12.8 ENSEMBL cdna:known chromosome:CEL140:II:2182511:2186422:-1 gene:C33C12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2182517-2186421(-) 99.53 100.0
Public Domain and Genome References
Gene Title glucosylceramidase
Ensembl C33C12.8
Entrez Gene 183155 Entrez gene
SwissProt O16581 EMBL-EBI
WormBase CE08556 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6665 sphingolipid metabolism inferred from electronic annotation QuickGO AmiGO
7040 lysosome organization and biogenesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5764 lysosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4348 glucosylceramidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494208 C33C12.8 [Caenorhabditis elegans] gb|AAB66060.1| Hypothetical protein C33C12.8 [Caenorhabditis elegans] 0.0
blast CAE70464 Hypothetical protein CBG17050 [Caenorhabditis briggsae] 0.0
blast CAE70469 Hypothetical protein CBG17056 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001139 EMBL-EBI Glycoside hydrolase, family 30 1.0E-126
Pfam IPR001139 EMBL-EBI Glycoside hydrolase, family 30 1.0E-126
Pfam IPR001139 EMBL-EBI Glycoside hydrolase, family 30 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:190002_AT
atgggcagacactgttctcaatgacaaggacgcagcaagttacgtctccggaatcgccgt
acactcttaccaggacgacgaaagtgacaaacatcttacacaaacccacaataaccaccc
agatgtgttcatttttggtacagaggcaagcgaggggtctaaatcgaaggatgtggatta
tggtagcttcgatcgagcagaagactatgtttcggacattttagatgatttcaataattg
ggttaccggatggacggagcgtaacctggtgctagatgctcaaggtggaccgtcctgggt
ttcgggattcgcggatgctccggtcattgcgttccccgcattggctcaattctacaagca
gcccatgttttatgcaattgcacattttagtcattttctgaagcccggagcagttcgaat
cgaccattcactgaacatgccaaacccagaaattgaacgaagtgcatttttgaatccgga
tggctcaaaagtcgtggtgctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGGCAGACACTGTTCTCAATGAC 270 49 1077 Antisense
GGACGCAGCAAGTTACGTCTCCGGA 12 525 1104 Antisense
CCGTACACTCTTACCAGGACGACGA 74 263 1133 Antisense
CAAACATCTTACACAAACCCACAAT 554 191 1164 Antisense
ATAACCACCCAGATGTGTTCATTTT 524 25 1187 Antisense
TGGACGGAGCGTAACCTGGTGCTAG 227 549 1327 Antisense
GCTAGATGCTCAAGGTGGACCGTCC 70 303 1347 Antisense
TCCTGGGTTTCGGGATTCGCGGATG 22 591 1369 Antisense
TAGTCATTTTCTGAAGCCCGGAGCA 495 649 1464 Antisense
GGAGCAGTTCGAATCGACCATTCAC 72 521 1483 Antisense
GATGGCTCAAAAGTCGTGGTGCTCC 112 407 1555 Antisense
Sequence Source Affymetrix Proprietary Database