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[189985_at] zinc metalloprotease

Description K03B8.2 /REP_DB=WormBase Gene ID /WP=CE06076 /TR=Q21178 /GB=CAA98501.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_total > 94      4grp_total > 17      6grp_cgc > 89      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 31.3
(-3)
A 		59.8
(0.2)
A 		31.8
(-2.9)
A 		84.6
(3)
M 		53
B 34
(0.3)
A 		8.6
(-3)
A 		54.3
(3)
A 		36.1
(0.6)
A 		46
C 6.9
(-2.8)
A 		40.5
(-0.4)
A 		4.1
(-3)
A 		87.8
(3)
P 		84
avr 24.1
(-3) 		36.3
(-1.4) 		30.1
(-2.2) 		69.5
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 10.4
(-1.6)
A 		2.7
(-3)
A 		8.6
(-2)
A 		6
(-2.4)
A 		36.8
(3)
A 		19.2
(-0.1)
A 		34
B 6.4
(-1.6)
A 		6.8
(-1.3)
A 		4.5
(-3)
A 		7.1
(-1.1)
A 		4.8
(-2.8)
A 		12.6
(3)
A 		8
C 4.5
(-2.8)
A 		24.2
(-0.4)
A 		2.6
(-3)
A 		8.6
(-2.3)
A 		51.9
(3)
A 		28.4
(0.1)
A 		49
avr 7.1
(-2.6) 		11.2
(-1.6) 		5.2
(-3) 		7.2
(-2.5) 		31.2
(3) 		20.1
(0.4) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 11.4
(1)
A 		13
(3)
A 		8.3
(-3)
A 		12.4
(2.2)
M 		5
B 10.8
(2)
A 		2.1
(-3)
A 		12.6
(3)
A 		7.7
(0.2)
A 		11
C 2.3
(-2.6)
A 		11.6
(1.6)
A 		1.4
(-3)
A 		14.8
(3)
P 		13
avr 8.2
(-1.9) 		8.9
(-0.9) 		7.4
(-3) 		11.6
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 1.9
(-2.5)
A 		1
(-3)
A 		1.9
(-2.5)
A 		1.5
(-2.7)
A 		11.1
(3)
A 		4.7
(-0.8)
A 		10
B 2.2
(-1.2)
A 		2.2
(-1.2)
A 		1.1
(-3)
A 		1.9
(-1.7)
A 		1.9
(-1.7)
A 		4.7
(3)
A 		4
C 1.7
(-2.6)
A 		7.4
(-0.5)
A 		0.7
(-3)
A 		1.7
(-2.6)
A 		16.8
(3)
A 		9.5
(0.3)
A 		16
avr 1.9
(-2.5) 		3.5
(-1.4) 		1.2
(-3) 		1.7
(-2.7) 		9.9
(3) 		6.3
(0.5) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189985_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13640
Sequence Type Exemplar sequence
Representative Public ID K03B8.2 NCBI
Target Description K03B8.2 /REP_DB=WormBase Gene ID /WP=CE06076 /TR=Q21178 /GB=CAA98501.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073491(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073491 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-17) (nas-17) mRNA, complete cds. 11/11 None
SNAP00000002780 ENSEMBL cdna:SNAP chromosome:CEL140:V:11401504:11403116:1 11/11 None
GENEFINDER00000002803 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11401531:11403116:1 11/11 None
K03B8.2 ENSEMBL cdna:known chromosome:CEL140:V:11401504:11403116:1 gene:K03B8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11401503-11403116(+) 100.0 100.0
Public Domain and Genome References
Gene Title zinc metalloprotease
Gene Symbol nas-17
Ensembl K03B8.2
Entrez Gene 186923 Entrez gene
SwissProt Q21178 EMBL-EBI
WormBase CE06076 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98501 Hypothetical protein K03B8.2 [Caenorhabditis elegans] ref|NP_505892.1| Nematode AStacin protease family member (nas-17) [Caenorhabditis elegans] 0.0
blast Q21178 Zinc metalloproteinase nas-17 precursor (Nematode astacin 17) 0.0
blast CAE75238 Hypothetical protein CBG23190 [Caenorhabditis briggsae] 1.0E-180
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.2E-88
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.2E-88
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505892.1 1 21-40
K03B8.2 1 21-40
SNAP00000002780 1 21-40
GENEFINDER00000002803 1 12-31
Sequence
Target Sequence 
>C. ELEGANS:189985_AT
gggattgcgtcgagtgtcattctacgatttggtcaatataaacgttcgatattcttgcgg
ttgtgctaagagtttgacatgtgagaatggtggatatacaaatccatccaattgtgctac
ttgtgtctgcccgactggatttgctggaactctttgcaacgaagcaccaagtaatactat
caagctcaccgcagaatcttattggaaaggatattgggttaattttgggtacagtacctc
aattcaaactaccaactattacttggcttacttgtggatcactgcgccagctgacaaaac
gattgaggttaagataatggatctctcaggtttcacatgctcttacggatgcaattacaa
tggagtggaggttaaatatatgggtgatccgagaattaccaatccactacgatgctgtgc
ccaagacactgaatatctgaaccaagtcatttccagcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGATTGCGTCGAGTGTCATTCTAC 309 495 762 Antisense
CGTTCGATATTCTTGCGGTTGTGCT 8 249 804 Antisense
TCCAATTGTGCTACTTGTGTCTGCC 508 587 868 Antisense
GGAACTCTTTGCAACGAAGCACCAA 21 531 907 Antisense
ATACTATCAAGCTCACCGCAGAATC 158 25 935 Antisense
TTACTTGTGGATCACTGCGCCAGCT 183 683 1029 Antisense
GATAATGGATCTCTCAGGTTTCACA 406 423 1074 Antisense
TCAGGTTTCACATGCTCTTACGGAT 186 605 1087 Antisense
GAATTACCAATCCACTACGATGCTG 34 325 1154 Antisense
TACGATGCTGTGCCCAAGACACTGA 235 647 1169 Antisense
ATCTGAACCAAGTCATTTCCAGCAA 153 35 1196 Antisense
Sequence Source Affymetrix Proprietary Database