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[189972_at] glucosylceramidase

Description C33C12.3 /REP_DB=WormBase Gene ID /WP=CE08551 /TR=O16580 /GB=AAB66056.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=glucosylceramidase
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 13      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 22      6grp_total > 13      4grp_total > 53      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1437
1 2 3 4 range
A 1393.3
(3)
P 		870.7
(0.2)
P 		944.2
(0.6)
P 		256.5
(-3)
P 		1137
B 1078.2
(3)
P 		936.4
(2)
P 		832.1
(1.3)
P 		210.8
(-3)
P 		867
C 1640.8
(3)
P 		723.7
(-0.8)
P 		761.3
(-0.7)
P 		204.2
(-3)
P 		1437
avr 1370.8
(3) 		843.6
(0.2) 		845.9
(0.3) 		223.8
(-3) 		1147

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1165
1 2 3 4 5 6 range
A 1830.6
(2.5)
P 		1913.7
(3)
P 		1436.1
(0.1)
P 		1323.1
(-0.5)
P 		968
(-2.7)
P 		909.9
(-3)
P 		1004
B 1890.5
(3)
P 		1665.3
(1.8)
P 		1251.3
(-0.4)
P 		1345.8
(0.1)
P 		1065.4
(-1.3)
P 		748.6
(-3)
P 		1142
C 1367.4
(1.1)
P 		1585.8
(3)
P 		1566.6
(2.8)
P 		1473.2
(2)
P 		943.1
(-2.5)
P 		889.3
(-3)
P 		697
avr 1696.2
(2.8) 		1721.6
(3) 		1418
(0.9) 		1380.7
(0.7) 		992.2
(-2) 		849.3
(-3) 		872
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 509
1 2 3 4 range
A 507.3
(3)
P 		189
(-1.1)
P 		246.8
(-0.3)
P 		37.5
(-3)
P 		470
B 343.8
(3)
P 		231.3
(0.7)
P 		192.8
(-0)
P 		44.9
(-3)
P 		299
C 543.1
(3)
P 		208.1
(-1)
P 		265.3
(-0.3)
P 		34.4
(-3)
P 		509
avr 464.7
(3) 		209.5
(-0.6) 		235
(-0.2) 		38.9
(-3) 		426

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 459
1 2 3 4 5 6 range
A 337.5
(-1.5)
P 		680.6
(3)
P 		312.7
(-1.8)
P 		335.6
(-1.5)
P 		292.1
(-2.1)
P 		221.5
(-3)
P 		459
B 657.8
(3)
P 		554.3
(1.4)
P 		303.8
(-2.6)
P 		356.5
(-1.8)
P 		432.9
(-0.6)
P 		279.1
(-3)
P 		379
C 506.8
(3)
P 		483.9
(2.3)
P 		398.7
(-0.1)
P 		296.3
(-3)
P 		305.2
(-2.7)
P 		297.7
(-3)
P 		211
avr 500.7
(1.6) 		572.9
(3) 		338.4
(-1.6) 		329.5
(-1.8) 		343.4
(-1.5) 		266.1
(-3) 		307
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189972_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4106
Sequence Type Exemplar sequence
Representative Public ID C33C12.3 NCBI
Target Description C33C12.3 /REP_DB=WormBase Gene ID /WP=CE08551 /TR=O16580 /GB=AAB66056.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=glucosylceramidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061806(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061806 NCBI Caenorhabditis elegans C33C12.3 (glucosylceramidase) mRNA, complete cds. 11/11 None
SNAP00000027204 ENSEMBL cdna:SNAP chromosome:CEL140:II:2170464:2194141:1 11/11 None
GENEFINDER00000027214 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2176948:2181886:1 11/11 None
C33C12.3 ENSEMBL cdna:known chromosome:CEL140:II:2176893:2181976:1 gene:C33C12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2176946-2181885(+) 96.53 100.0
Public Domain and Genome References
Gene Title glucosylceramidase
Ensembl C33C12.3
Entrez Gene 173574 Entrez gene
SwissProt O16580 EMBL-EBI
WormBase CE29208 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6665 sphingolipid metabolism inferred from electronic annotation QuickGO AmiGO
7040 lysosome organization and biogenesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5764 lysosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4348 glucosylceramidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494207 C33C12.3 [Caenorhabditis elegans] gb|AAB66056.2| Hypothetical protein C33C12.3 [Caenorhabditis elegans] 0.0
blast CAE70469 Hypothetical protein CBG17056 [Caenorhabditis briggsae] 0.0
blast CAE70464 Hypothetical protein CBG17050 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001139 EMBL-EBI Glycoside hydrolase, family 30 1.0E-126
Pfam IPR001139 EMBL-EBI Glycoside hydrolase, family 30 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189972_AT
gcttccgaaggctacgggtccaaggacacccacgtggattatggaaattgggatcgtgca
gcagatactgcttcagatattttagatgtaagagaaaatatagttttgaagaaatttcta
gaaaattggggaaaagttcggaaaaaaaatttcaaaattttaatttttttttcaaatttt
ttttttcataatttttttttctacatttttttccagaacatgaacaactggatgactggc
tggacagaacgtaacctaatcctagacgccctgggtggtccatcatgggtgtccgactac
acagatgcgccggtcattgcgttcccagcgatggctcaattctacaagcagcccatgttt
tatgcgattgcacattttagtcacttcatcaagcctggagcagtaagaatcgaccactca
ttaaatgtaattgagctggaagttgaaaccaccgcatttttgaatcctgatggctcaaaa
gttattgtaatgctcaataagggctcgctagtctcaacggagcacacagtagttgtccag
gatgctgctgatagccgtaatcactatcattttacacttccacatcgagctattacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTCCGAAGGCTACGGGTCCAAGG 348 305 1087 Antisense
GACAGAACGTAACCTAATCCTAGAC 433 361 1329 Antisense
GCCCTGGGTGGTCCATCATGGGTGT 242 287 1354 Antisense
ACACAGATGCGCCGGTCATTGCGTT 475 111 1385 Antisense
TTCCCAGCGATGGCTCAATTCTACA 432 697 1408 Antisense
AACCACCGCATTTTTGAATCCTGAT 321 147 1533 Antisense
GGCTCGCTAGTCTCAACGGAGCACA 622 539 1588 Antisense
CGGAGCACACAGTAGTTGTCCAGGA 270 243 1604 Antisense
GTCCAGGATGCTGCTGATAGCCGTA 542 475 1621 Antisense
ATCACTATCATTTTACACTTCCACA 76 29 1646 Antisense
TACACTTCCACATCGAGCTATTACC 660 641 1659 Antisense
Sequence Source Affymetrix Proprietary Database