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[189971_at] enoyl-CoA hydratase

Description F01G10.3 /REP_DB=WormBase Gene ID /WP=CE09165 /TR=O17762 /GB=CAB02892.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=enoyl-CoA hydratase
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 82      4grp_highest_8_8 > 50      4grp_high_8_8 > 32      6grp_highest_nonorm > 71      6grp_total > 70      4grp_total > 47      6grp_highest_sub > 04      6grp_cgc > 95      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1531
1 2 3 4 range
A 1376.5
(3)
P 		144.7
(-2.8)
P 		151.6
(-2.8)
P 		104.5
(-3)
P 		1272
B 1249.4
(3)
P 		96.5
(-2.9)
P 		152.2
(-2.6)
P 		71
(-3)
P 		1178
C 1601.9
(3)
P 		99.3
(-3)
P 		147.4
(-2.8)
P 		110.2
(-3)
P 		1503
avr 1409.3
(3) 		113.5
(-2.9) 		150.4
(-2.7) 		95.2
(-3) 		1314

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 460
1 2 3 4 5 6 range
A 312.4
(-0.8)
P 		173.1
(-3)
P 		385.2
(0.3)
P 		557.4
(3)
P 		286.4
(-1.2)
P 		271.9
(-1.5)
P 		384
B 345
(-1)
P 		254.3
(-3)
P 		265.5
(-2.8)
P 		524.5
(3)
P 		279.3
(-2.4)
P 		382.1
(-0.2)
P 		270
C 243.5
(-2.4)
P 		256.1
(-2.2)
P 		329.6
(-1.2)
P 		632.9
(3)
P 		196.4
(-3)
P 		276.3
(-1.9)
P 		437
avr 300.3
(-1.7) 		227.8
(-3) 		326.8
(-1.3) 		571.6
(3) 		254
(-2.5) 		310.1
(-1.6) 		344
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 515
1 2 3 4 range
A 501.2
(3)
P 		31.4
(-2.8)
P 		39.6
(-2.7)
P 		15.3
(-3)
P 		486
B 398.4
(3)
P 		23.8
(-2.9)
P 		35.3
(-2.7)
P 		15.1
(-3)
P 		383
C 530.3
(3)
P 		28.6
(-2.9)
P 		51.4
(-2.6)
P 		18.6
(-3)
P 		512
avr 476.6
(3) 		27.9
(-2.8) 		42.1
(-2.7) 		16.3
(-3) 		460

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 57.6
(-3)
P 		61.6
(-2.7)
P 		83.9
(-1.1)
P 		141.4
(3)
P 		86.4
(-0.9)
P 		66.2
(-2.4)
P 		84
B 120
(1.3)
P 		84.6
(-1.5)
P 		64.5
(-3)
P 		138.9
(2.7)
P 		113.5
(0.8)
P 		142.5
(3)
P 		78
C 90.2
(-0.5)
P 		78.2
(-1.6)
P 		83.9
(-1.1)
P 		127.3
(3)
P 		63.6
(-3)
P 		92.5
(-0.3)
P 		64
avr 89.3
(-1.6) 		74.8
(-3) 		77.4
(-2.7) 		135.9
(3) 		87.8
(-1.7) 		100.4
(-0.5) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189971_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9963
Sequence Type Exemplar sequence
Representative Public ID F01G10.3 NCBI
Target Description F01G10.3 /REP_DB=WormBase Gene ID /WP=CE09165 /TR=O17762 /GB=CAB02892.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=enoyl-CoA hydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069474(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069474 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-9) (ech-9) mRNA, complete cds. 11/11 None
SNAP00000013158 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10233607:10235281:-1 11/11 None
GENEFINDER00000013165 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10233607:10235281:-1 11/11 None
F01G10.3 ENSEMBL cdna:known chromosome:CEL140:IV:10233292:10235300:-1 gene:F01G10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10233612-10235096(-) 100.0 100.0
Public Domain and Genome References
Gene Title enoyl-CoA hydratase
Gene Symbol ech-9
Ensembl F01G10.3
Entrez Gene 184065 Entrez gene
SwissProt O17762 EMBL-EBI
WormBase CE35451 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6631 fatty acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02892 Hypothetical protein F01G10.3 [Caenorhabditis elegans] ref|NP_501875.2| Enoyl-CoA Hydratase family member (ech-9) [Caenorhabditis elegans] 0.0
blast CAE59904 Hypothetical protein CBG03388 [Caenorhabditis briggsae] 0.0
blast CAB02891 Hypothetical protein F01G10.2 [Caenorhabditis elegans] ref|NP_501876.2| Enoyl-CoA Hydratase family member (ech-8) [Caenorhabditis elegans] 1.0E-172
Protein Domains
Database ID Description E-Value
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 1.4E-13
Pfam IPR006176 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain 1.1E-55
Sequence
Target Sequence 
>C. ELEGANS:189971_AT
ggataccttccacatcaaattgataaaattatcacgaattttggatttttgatgggacca
atgactgttgccgatatgaatggatttgatgtaatggaaaagttgaaaaaggaaaatgga
cttgagccgaatccgattgaaaaggaaatgtggagactgaaaagatacggacggaaaact
aacaaaggattttacaaatatgacgataaaacccaacggaaagaaaatgacactgaaatg
gaacagattattcgtcgagtttctcaaaatgccaaatctaacattcaaattataaatgat
caggatgtgattaattttatgttgtatcctactgtaaatgaaggttaccggtgtattgaa
gaaggtgttatcagtaatgaaagtttaattgatatcatgtttattctcggttttggttgg
cctattcatagtggtggaccgatgagatttggtaaaactgaaggacttgataaaattgca
aatatgcttgttcactggagttccttggagcccaaggaaagtgcttatattgttgctgac
gctcttaaaactgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATACCTTCCACATCAAATTGATA 505 507 586 Antisense
AATGGACTTGAGCCGAATCCGATTG 2 163 700 Antisense
ACAGATTATTCGTCGAGTTTCTCAA 659 107 828 Antisense
TTAATTTTATGTTGTATCCTACTGT 311 685 896 Antisense
GATATCATGTTTATTCTCGGTTTTG 522 425 976 Antisense
TTCTCGGTTTTGGTTGGCCTATTCA 176 687 989 Antisense
GGCCTATTCATAGTGGTGGACCGAT 688 543 1004 Antisense
AATATGCTTGTTCACTGGAGTTCCT 164 173 1066 Antisense
TCACTGGAGTTCCTTGGAGCCCAAG 229 627 1077 Antisense
GTGCTTATATTGTTGCTGACGCTCT 432 479 1106 Antisense
TTGTTGCTGACGCTCTTAAAACTGC 136 695 1115 Antisense
Sequence Source Affymetrix Proprietary Database