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[189955_at] acetly-CoA thiolase

Description T02G5.4 /REP_DB=WormBase Gene ID /WP=CE04856 /TR=Q22106 /GB=AAA82403.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=acetly-CoA thiolase
Cluster 6grp_total > 93      4grp_total > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 40.9
(-2.4)
A 		46.6
(-2.2)
A 		28.8
(-3)
A 		155.7
(3)
P 		127
B 54
(3)
P 		50.8
(0.5)
A 		46.4
(-3)
P 		47.5
(-2.1)
A 		8
C 30.6
(-1.7)
A 		17.5
(-3)
A 		31.9
(-1.6)
P 		77.3
(3)
A 		60
avr 41.8
(-2.4) 		38.3
(-2.7) 		35.7
(-3) 		93.5
(3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 27.1
(-3)
A 		44.9
(-0.8)
A 		50
(-0.2)
A 		45.8
(-0.7)
A 		76.3
(3)
M 		49.6
(-0.3)
A 		49
B 50.9
(3)
A 		41.1
(1.5)
A 		38.3
(1.1)
A 		10.5
(-3)
A 		48.2
(2.6)
P 		35.8
(0.8)
P 		40
C 49.3
(3)
A 		49.1
(2.9)
A 		42
(1)
A 		27.5
(-3)
A 		43.6
(1.4)
M 		43.5
(1.4)
A 		22
avr 42.4
(0.1) 		45
(0.7) 		43.4
(0.3) 		27.9
(-3) 		56
(3) 		43
(0.2) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 14.9
(-0.1)
A 		10.1
(-2)
A 		7.5
(-3)
A 		22.8
(3)
P 		15
B 17.2
(3)
P 		12.5
(-1)
A 		10.8
(-2.4)
P 		10.1
(-3)
A 		7
C 10.1
(0.8)
A 		5
(-3)
A 		11.1
(1.6)
P 		13
(3)
A 		8
avr 14.1
(1.8) 		9.2
(-3) 		9.8
(-2.4) 		15.3
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 5
(-3)
A 		16
(0.7)
A 		10.9
(-1)
A 		11.6
(-0.8)
A 		23
(3)
M 		12.1
(-0.6)
A 		18
B 17.7
(2.3)
A 		13.7
(0.9)
A 		9.3
(-0.7)
A 		2.8
(-3)
A 		19.6
(3)
P 		13.3
(0.8)
P 		17
C 18.3
(3)
A 		15
(1.5)
A 		10.7
(-0.6)
A 		5.5
(-3)
A 		14.1
(1)
M 		14.6
(1.3)
A 		13
avr 13.7
(0.5) 		14.9
(1) 		10.3
(-1.2) 		6.6
(-3) 		18.9
(3) 		13.3
(0.3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189955_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4499
Sequence Type Exemplar sequence
Representative Public ID T02G5.4 NCBI
Target Description T02G5.4 /REP_DB=WormBase Gene ID /WP=CE04856 /TR=Q22106 /GB=AAA82403.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=acetly-CoA thiolase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063060(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063060 NCBI Caenorhabditis elegans T02G5.4 (T02G5.4) mRNA, complete cds. 11/11 A 
SNAP00000014288 ENSEMBL cdna:SNAP chromosome:CEL140:II:7082032:7084013:1 11/11 None
GENEFINDER00000014295 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7082032:7088142:1 11/11 None
T02G5.4 ENSEMBL cdna:known chromosome:CEL140:II:7082032:7084304:1 gene:T02G5.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7082027-7084009(+) 99.35 100.0
Public Domain and Genome References
Gene Title acetly-CoA thiolase
Ensembl T02G5.4
Entrez Gene 187992 Entrez gene
SwissProt Q22106 EMBL-EBI
WormBase CE04856 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495461 T02G5.4 [Caenorhabditis elegans] gb|AAA82403.1| Hypothetical protein T02G5.4 [Caenorhabditis elegans] 0.0
blast NP_495455 3-Ketoacyl-coA Thiolase family member (kat-1) [Caenorhabditis elegans] gb|AAA82397.2| 3-ketoacyl-coa thiolase protein 1 [Caenorhabditis elegans] 1.0E-142
blast NP_495455 3-Ketoacyl-coA Thiolase family member (kat-1) [Caenorhabditis elegans] gb|AAA82397.2| 3-ketoacyl-coa thiolase protein 1 [Caenorhabditis elegans] 1.0E-138
blast CAE56900 Hypothetical protein CBG24741 [Caenorhabditis briggsae] 1.0E-138
blast CAE56900 Hypothetical protein CBG24741 [Caenorhabditis briggsae] 1.0E-135
Protein Domains
Database ID Description E-Value
Pfam IPR002155 EMBL-EBI Thiolase 4.6E-44
Pfam IPR002155 EMBL-EBI Thiolase 9.5E-51
Pfam IPR002155 EMBL-EBI Thiolase 4.6E-44
Pfam IPR002155 EMBL-EBI Thiolase 3.2E-48
Sequence
Target Sequence 
>C. ELEGANS:189955_AT
gaactaacggcaacattggtccagaagtggtggcagtgaacgtaaaatcgaagaaagggg
tggaagccgtcaaacaagatgacattacgaagatcaagaagatcaaggaaggctcagtgt
attcaagcattccaatttttccaggcggaacaatttcggataaacacattgccgcgttca
cagacggagcagctgctgtcattcttgcctcgcaggaagcagtttccgagcaaaacttga
agccattggccagaattttggcgtacggagatgccgccacgcaccaactcgatttcgctg
tagcgccgactttgctgttcccaaatcttcttcaatcagcaggagtcgagcaatcagatg
ttgctcaatgggaagttaatgaagccttctcatgtgttccccttgctttcatcaaaaaac
taggagtcgatccatcacttgtgaacccacatggaggagctgtttcaattggtcacccca
tcggaatgtccggagccagcctcatcactcatcttgtgcacacactcgaaagtggccaaa
tcggagttgctgccctttgtgacggagacggtgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTAACGGCAACATTGGTCCAGA 237 349 791 Antisense
AGGGGTGGAAGCCGTCAAACAAGAT 247 57 846 Antisense
GGCTCAGTGTATTCAAGCATTCCAA 475 539 901 Antisense
TTTCCAGGCGGAACAATTTCGGATA 375 669 928 Antisense
CGGATAAACACATTGCCGCGTTCAC 533 243 947 Antisense
CGCGTTCACAGACGGAGCAGCTGCT 384 253 963 Antisense
TGTCATTCTTGCCTCGCAGGAAGCA 105 559 987 Antisense
GCCATTGGCCAGAATTTTGGCGTAC 606 281 1032 Antisense
TTTGGCGTACGGAGATGCCGCCACG 281 663 1047 Antisense
CAATCAGCAGGAGTCGAGCAATCAG 263 181 1123 Antisense
CTGCCCTTTGTGACGGAGACGGTGG 575 235 1340 Antisense
Sequence Source Affymetrix Proprietary Database