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[189953_at] zinc metalloprotease

Description T11F9.3 /REP_DB=WormBase Gene ID /WP=CE06415 /TR=Q22396 /GB=CAA98528.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_total > 93      4grp_total > 20      6grp_cgc > 99      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 14
(-2.1)
A 		14.6
(-2)
A 		8.6
(-3)
A 		45.7
(3)
A 		37
B 40.9
(3)
A 		9.9
(-3)
A 		36.5
(2.1)
A 		20.1
(-1)
A 		31
C 33.8
(-0.1)
A 		65.2
(3)
A 		5
(-3)
A 		64.3
(2.9)
A 		60
avr 29.6
(-0.1) 		29.9
(-0) 		16.7
(-3) 		43.4
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 171
1 2 3 4 5 6 range
A 33.3
(-2.4)
A 		16.9
(-3)
A 		43.6
(-2)
A 		30.6
(-2.5)
A 		185.1
(3)
P 		55.8
(-1.6)
A 		168
B 30.2
(-2.4)
A 		26.1
(-2.6)
A 		14.3
(-3)
A 		37
(-2.1)
A 		173.5
(3)
P 		43.1
(-1.9)
P 		159
C 18.6
(-3)
A 		21.9
(-2.8)
A 		50.4
(-1.5)
A 		70.5
(-0.6)
A 		146.8
(3)
P 		42.7
(-1.9)
P 		128
avr 27.4
(-2.8) 		21.6
(-3) 		36.1
(-2.4) 		46
(-2) 		168.5
(3) 		47.2
(-2) 		147
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 5.1
(0.9)
A 		3.2
(-1.7)
A 		2.2
(-3)
A 		6.7
(3)
A 		5
B 13
(3)
A 		2.4
(-3)
A 		8.4
(0.4)
A 		4.3
(-1.9)
A 		11
C 11.2
(0.4)
A 		18.7
(3)
A 		1.7
(-3)
A 		10.8
(0.2)
A 		17
avr 9.8
(3) 		8.1
(1.2) 		4.1
(-3) 		7.3
(0.4) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 6.1
(-3)
A 		6
(-3)
A 		9.5
(-2.6)
A 		7.8
(-2.8)
A 		55.8
(3)
P 		13.6
(-2.1)
A 		50
B 10.5
(-2.4)
A 		8.7
(-2.5)
A 		3.5
(-3)
A 		9.8
(-2.4)
A 		70.5
(3)
P 		16.1
(-1.9)
P 		67
C 6.9
(-3)
A 		6.7
(-3)
A 		12.8
(-2.1)
A 		14.2
(-1.9)
A 		47.5
(3)
P 		14.3
(-1.9)
P 		41
avr 7.8
(-2.9) 		7.1
(-3) 		8.6
(-2.8) 		10.6
(-2.6) 		57.9
(3) 		14.7
(-2.1) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189953_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13351
Sequence Type Exemplar sequence
Representative Public ID T11F9.3 NCBI
Target Description T11F9.3 /REP_DB=WormBase Gene ID /WP=CE06415 /TR=Q22396 /GB=CAA98528.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073505(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073505 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-20) (nas-20) mRNA, complete cds. 11/11 None
SNAP00000013919 ENSEMBL cdna:SNAP chromosome:CEL140:V:11473554:11474624:-1 11/11 None
GENEFINDER00000013940 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11473554:11474883:-1 11/11 None
T11F9.3 ENSEMBL cdna:known chromosome:CEL140:V:11473554:11474883:-1 gene:T11F9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11473553-11474624(-) 100.0 100.0
Public Domain and Genome References
Gene Title zinc metalloprotease
Gene Symbol nas-20
Ensembl T11F9.3
Entrez Gene 188420 Entrez gene
SwissProt Q22396 EMBL-EBI
WormBase CE35907 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98528 Hypothetical protein T11F9.3 [Caenorhabditis elegans] ref|NP_505906.2| Nematode AStacin protease family member (nas-20) [Caenorhabditis elegans] sp|Q22396|NAS20_CAEEL Zinc metalloproteinase nas-20 precursor (Nematode astacin 20) 0.0
blast CAE75252 Hypothetical protein CBG23211 [Caenorhabditis briggsae] 1.0E-130
blast CAE75253 Hypothetical protein CBG23213 [Caenorhabditis briggsae] 1.0E-129
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 7.7E-35
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 1.2E-10
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 1.3E-33
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 1.2E-10
Sequence
Target Sequence 
>C. ELEGANS:189953_AT
gggaagccttcgagttgcgttttatgatatggttttattgaacaaattctatggctgcaa
ctgtgataatcatccaagaaaattggattgtaaaaatggcggctatcagaatccggccaa
ctgtgaagaatgtctttgtactgacggattcaatggtcaattatgcgatcagcatgaagg
agtttacgttttggaagctaaaaaagaatgggatgcatcaggagtgcgtaataattacag
aaaaggaattgaaacaaatactatgccggagtatacttattttgcattgactgctccaga
aggatccacaattgaggttcgtatcaccaaactatccggatttttctgtcaacatacatg
cgattataatggtgttgaattgaaatacaaaactgatcgacgaattgtgagcccattagt
atgctgtgataatgataatttatggaataagacacgatcatcaaccaataatccattcat
tattgcaaaatatggaaataaccgaacacctcactttgaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGAAGCCTTCGAGTTGCGTTTTAT 69 241 408 Antisense
AAATTCTATGGCTGCAACTGTGATA 82 117 451 Antisense
AAATGGCGGCTATCAGAATCCGGCC 552 119 501 Antisense
AATCCGGCCAACTGTGAAGAATGTC 308 165 517 Antisense
ACTTATTTTGCATTGACTGCTCCAG 265 101 682 Antisense
AAGGATCCACAATTGAGGTTCGTAT 185 159 707 Antisense
GAGGTTCGTATCACCAAACTATCCG 527 403 721 Antisense
ACTATCCGGATTTTTCTGTCAACAT 366 101 738 Antisense
ACTGATCGACGAATTGTGAGCCCAT 365 97 799 Antisense
GTGAGCCCATTAGTATGCTGTGATA 606 483 814 Antisense
ATAACCGAACACCTCACTTTGAATT 627 23 905 Antisense
Sequence Source Affymetrix Proprietary Database