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[189946_at]

Description T25C8.3 /REP_DB=WormBase Gene ID /WP=CE18965 /TR=Q9TVH2 /GB=CAB05820.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Glutamine synthetase
Cluster 6grp_high1_10_10 > 79      4grp_high_8_8 > 36      6grp_total > 79      4grp_total > 51      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 71.1
(-0)
P 		110.7
(3)
P 		68.3
(-0.2)
P 		32.3
(-3)
A 		78
B 78.9
(-0.2)
P 		76.6
(-0.5)
P 		100.1
(3)
P 		59.8
(-3)
P 		40
C 105.1
(-0.7)
P 		150.5
(3)
P 		77.6
(-3)
P 		80.8
(-2.7)
P 		73
avr 85
(-0) 		112.6
(3) 		82
(-0.3) 		57.6
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 105.6
(2.7)
P 		55.9
(-2.6)
P 		68.3
(-1.2)
A 		51.7
(-3)
P 		84.8
(0.5)
P 		108.4
(3)
P 		57
B 65.5
(-0.7)
P 		47.8
(-2.2)
P 		68
(-0.4)
P 		39.2
(-3)
A 		106.6
(3)
P 		104.4
(2.8)
P 		67
C 61.4
(-2.4)
P 		65.9
(-1.9)
P 		58.2
(-2.8)
P 		56.3
(-3)
A 		66
(-1.9)
P 		109.4
(3)
P 		53
avr 77.5
(-0.1) 		56.5
(-2.2) 		64.8
(-1.4) 		49.1
(-3) 		85.8
(0.8) 		107.4
(3) 		58
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 25.9
(3)
P 		24
(2.5)
P 		17.9
(0.7)
P 		4.7
(-3)
A 		21
B 25.2
(3)
P 		18.9
(-0)
P 		23.2
(2)
P 		12.7
(-3)
P 		13
C 34.8
(1.3)
P 		43.3
(3)
P 		27
(-0.3)
P 		13.6
(-3)
P 		30
avr 28.6
(3) 		28.7
(3) 		22.7
(1) 		10.3
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 19.5
(-0.1)
P 		19.9
(0.1)
P 		14.9
(-2.2)
A 		13.1
(-3)
P 		25.6
(2.6)
P 		26.4
(3)
P 		13
B 22.8
(-0.7)
P 		15.9
(-2)
P 		16.5
(-1.9)
P 		10.4
(-3)
A 		43.3
(3)
P 		38.9
(2.2)
P 		33
C 22.8
(-0.3)
P 		20.1
(-0.9)
P 		14.8
(-2.2)
P 		11.3
(-3)
A 		21.3
(-0.6)
P 		36.6
(3)
P 		25
avr 21.7
(-0.3) 		18.6
(-1.1) 		15.4
(-2) 		11.6
(-3) 		30.1
(2) 		34
(3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189946_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7600
Sequence Type Exemplar sequence
Representative Public ID T25C8.3 NCBI
Target Description T25C8.3 /REP_DB=WormBase Gene ID /WP=CE18965 /TR=Q9TVH2 /GB=CAB05820.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Glutamine synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description Only library file information is available for this probe set. See probe design information above.
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
T25C8.3 ENSEMBL cdna:known chromosome:CEL140:III:13601499:13602665:-1 gene:T25C8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13601494-13602661(-) 100.0 100.0
Public Domain and Genome References
Ensembl T25C8.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast A88616 protein T25C8.3 [imported] - Caenorhabditis elegans 1.0E-177
blast CAA82655 Hypothetical protein K03H1.1 [Caenorhabditis elegans] ref|NP_499208.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-2) [Caenorhabditis elegans] sp|P34497|GLNA_CAEEL Probable glutamine synthetase (Glutamate--ammonia ligase) 1.0E-158
blast CAE73310 Hypothetical protein CBG20737 [Caenorhabditis briggsae] 1.0E-157
Protein Domains
Database ID Description E-Value
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 5.1E-34
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 2.0E-56
Sequence
Target Sequence 
>C. ELEGANS:189946_AT
tgcctatccggatccgtttttgggcggtgggaataagctggtgatgtgtgacacgctgga
ccatcaaatgcagccaacagcaaccagtcatcgtcaagcatgcgccgaaatcatgaacga
aatccgtgatacccgcccatggtttggtatggagcaggaatatctgatcgtcgacagaga
tgagcactcactaggatggccaaaacacggattcccggctccacaaggaaagtactactg
tagcgtcggagcggatcgtgcgttcgggagagaagtcgttgagacacattatcgtgcttg
ccttcacgccgggctcaatattttcggaacaaatgctgaagtgacgcctggacaatggga
attccaaatcggaacctgcgaaggaatcgatatgggagatcaattgtggatgtcgagata
tattttgcacagagttgctgaacaattcggtgtctgcgtatctcttgatcccaaaccaaa
ggtcaccatgggagattggaacggagccagatgccataccaacttctccacgctgaaatg
cgtgctccaggcggtatcgccgcgattgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCCTATCCGGATCCGTTTTTGGGC 267 583 273 Antisense
GTGACACGCTGGACCATCAAATGCA 19 483 320 Antisense
CAACCAGTCATCGTCAAGCATGCGC 81 187 353 Antisense
GATACCCGCCCATGGTTTGGTATGG 408 325 400 Antisense
GATGAGCACTCACTAGGATGGCCAA 467 411 451 Antisense
GATTCCCGGCTCCACAAGGAAAGTA 499 431 482 Antisense
TACTGTAGCGTCGGAGCGGATCGTG 461 645 508 Antisense
GAGACACATTATCGTGCTTGCCTTC 452 393 553 Antisense
AGTGACGCCTGGACAATGGGAATTC 433 59 612 Antisense
TGGAACGGAGCCAGATGCCATACCA 420 545 769 Antisense
TCCAGGCGGTATCGCCGCGATTGAA 401 589 818 Antisense
Sequence Source Affymetrix Proprietary Database