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[189937_at] zinc metalloprotease

Description T11F9.6 /REP_DB=WormBase Gene ID /WP=CE06418 /TR=Q22398 /GB=CAA98530.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_total > 99      4grp_total > 33      6grp_cgc > 89      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 1
(-3)
A 		3.3
(-0.2)
A 		4.9
(1.7)
A 		6
(3)
A 		5
B 28.7
(3)
A 		2.1
(-3)
A 		10.7
(-1.1)
A 		12.7
(-0.6)
A 		27
C 1.7
(-3)
A 		31.7
(3)
A 		20.2
(0.7)
A 		19.4
(0.5)
A 		30
avr 10.5
(-3) 		12.4
(2.2) 		11.9
(0.8) 		12.7
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 2.9
(-3)
A 		21.2
(-0.1)
A 		2.8
(-3)
A 		2.7
(-3)
A 		39
(2.7)
A 		40.9
(3)
A 		38
B 2.1
(-3)
A 		21.1
(-0.7)
A 		12.1
(-1.8)
A 		1.7
(-3)
A 		52.6
(3)
P 		27
(-0)
M 		51
C 4.6
(-2.6)
A 		16.5
(-1.1)
A 		1.2
(-3)
A 		6.7
(-2.3)
A 		50.8
(3)
A 		44.1
(2.2)
P 		50
avr 3.2
(-3) 		19.6
(-0.8) 		5.4
(-2.7) 		3.7
(-2.9) 		47.5
(3) 		37.3
(1.6) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 0.4
(-3)
A 		0.7
(-1)
A 		1.3
(3)
A 		0.9
(0.3)
A 		1
B 9.2
(3)
A 		0.5
(-3)
A 		2.5
(-1.6)
A 		2.7
(-1.5)
A 		9
C 0.6
(-3)
A 		9.1
(3)
A 		7
(1.5)
A 		3.3
(-1.1)
A 		9
avr 3.4
(2.1) 		3.4
(2.1) 		3.6
(3) 		2.3
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 0.5
(-3)
A 		7.6
(0.8)
A 		0.6
(-2.9)
A 		0.7
(-2.9)
A 		11.8
(3)
A 		9.9
(2)
A 		11
B 0.7
(-2.9)
A 		7
(-1.1)
A 		2.9
(-2.3)
A 		0.5
(-3)
A 		21.4
(3)
P 		10.1
(-0.2)
M 		21
C 1.7
(-2.5)
A 		5
(-1.2)
A 		0.3
(-3)
A 		1.3
(-2.6)
A 		16.4
(3)
A 		14.8
(2.4)
P 		16
avr 1
(-2.9) 		6.5
(-0.8) 		1.3
(-2.8) 		0.8
(-3) 		16.5
(3) 		11.6
(1.1) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189937_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13565
Sequence Type Exemplar sequence
Representative Public ID T11F9.6 NCBI
Target Description T11F9.6 /REP_DB=WormBase Gene ID /WP=CE06418 /TR=Q22398 /GB=CAA98530.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073507(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073507 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-22) (nas-22) mRNA, complete cds. 11/11 None
SNAP00000013921 ENSEMBL cdna:SNAP chromosome:CEL140:V:11477299:11477944:1 11/11 None
T11F9.6 ENSEMBL cdna:known chromosome:CEL140:V:11477299:11477944:1 gene:T11F9.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11477298-11477944(+) 100.0 100.0
Public Domain and Genome References
Gene Title zinc metalloprotease
Gene Symbol nas-22
Ensembl T11F9.6
Entrez Gene 188423 Entrez gene
SwissProt Q22398 EMBL-EBI
WormBase CE06418 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98530 Hypothetical protein T11F9.6 [Caenorhabditis elegans] ref|NP_505908.1| Nematode AStacin protease family member (nas-22) [Caenorhabditis elegans] 1.0E-114
blast Q22398 Zinc metalloproteinase nas-22 precursor (Nematode astacin 22) 1.0E-114
blast CAE75254 Hypothetical protein CBG23214 [Caenorhabditis briggsae] 8.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 2.3E-16
Sequence
Target Sequence 
>C. ELEGANS:189937_AT
gaagtcgttttttattcttctctcaattttacaagagtgctatggaaaggatattgttgc
aagaattggtggaagaaatgtcgccgaaaaaattggtggagcccgtcataggagacaagt
attgattcgtgggtcagatgaagagcgacgacataaatggttcaacaatacagtacatta
ttatttttatgaagaaaatttcgatttcactgtgaaagagtcaatacttcgtgcaatgga
attgatatccaatcatacctgcattaaattctcaacagaaccatctgaaaaatcaattcg
aatggaaagtgattccacgacaatcgcatgttatgcagaaattggtcaggtccgagagaa
tcaactttttagtttcaactcagactgctattctgctggtgttgccgttcatgaacttat
tcacagtttgggatttattcatgcacatcagagatccgatagagatcagtatctcgaatt
caagaaaaatctcgatgaattaaaccaaacttatcaggaacaatacaaaatttgggaata
tcaagagatacttgttccatatgatgttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTCGTTTTTTATTCTTCTCTCA 7 337 15 Antisense
ATTGGTGGAGCCCGTCATAGGAGAC 680 3 106 Antisense
GAAAGAGTCAATACTTCGTGCAATG 531 359 228 Antisense
GATATCCAATCATACCTGCATTAAA 621 425 258 Antisense
GTGATTCCACGACAATCGCATGTTA 308 483 323 Antisense
GGTCAGGTCCGAGAGAATCAACTTT 636 499 358 Antisense
TTTAGTTTCAACTCAGACTGCTATT 65 673 382 Antisense
CAGACTGCTATTCTGCTGGTGTTGC 344 205 395 Antisense
GGTGTTGCCGTTCATGAACTTATTC 90 501 412 Antisense
TATTCATGCACATCAGAGATCCGAT 661 663 450 Antisense
GAGATACTTGTTCCATATGATGTTG 161 397 559 Antisense
Sequence Source Affymetrix Proprietary Database