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[189932_s_at] actin binding domain

Description F25B3.1 /REP_DB=WormBase Gene ID /WP=CE05711 /TR=Q18749 /GB=CAA94758.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=actin binding domain
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 65      4grp_highest_8_8 > 36      4grp_high_8_8 > 0      6grp_highest_nonorm > 75      6grp_total > 65      4grp_total > 15      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 319
1 2 3 4 range
A 718.8
(3)
P 		450.7
(-3)
P 		635
(1.1)
P 		651.9
(1.5)
P 		268
B 770
(3)
P 		579.2
(-3)
P 		612
(-2)
P 		762.5
(2.8)
P 		191
C 717.9
(3)
P 		581.2
(-3)
P 		617.1
(-1.4)
P 		604.6
(-2)
P 		137
avr 735.6
(3) 		537
(-3) 		621.4
(-0.5) 		673
(1.1) 		199

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 359
1 2 3 4 5 6 range
A 910.9
(0.6)
P 		843
(-2.3)
P 		834.2
(-2.6)
P 		945.8
(2.1)
P 		825.2
(-3)
P 		968.1
(3)
P 		143
B 829.7
(-3)
P 		848.9
(-2.3)
P 		993.7
(3)
P 		891.7
(-0.7)
P 		898.4
(-0.5)
P 		949.9
(1.4)
P 		164
C 791
(0)
P 		895
(2.1)
P 		858.5
(1.4)
P 		909.8
(2.3)
P 		634.5
(-3)
P 		943.3
(3)
P 		309
avr 843.9
(-0.9) 		862.3
(-0.3) 		895.5
(0.9) 		915.8
(1.6) 		786
(-3) 		953.8
(3) 		168
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 261.7
(3)
P 		97.8
(-2.9)
P 		166
(-0.5)
P 		95.4
(-3)
P 		166
B 245.5
(3)
P 		143
(-2.9)
P 		141.8
(-3)
P 		162.5
(-1.8)
P 		104
C 237.6
(3)
P 		167.1
(-0.1)
P 		215.1
(2)
P 		101.9
(-3)
P 		136
avr 248.3
(3) 		136
(-2.2) 		174.3
(-0.5) 		119.9
(-3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 197
1 2 3 4 5 6 range
A 167.9
(-3)
P 		299.8
(3)
P 		181.7
(-2.4)
P 		239.9
(0.3)
P 		249
(0.7)
P 		235.7
(0.1)
P 		132
B 288.7
(-0.6)
P 		282.6
(-0.8)
P 		241.3
(-2.8)
P 		236.2
(-3)
P 		365
(3)
P 		354.2
(2.5)
P 		129
C 293.2
(2)
P 		273.1
(1.1)
P 		218.5
(-1.4)
P 		183
(-3)
P 		205.3
(-2)
P 		315.8
(3)
P 		133
avr 249.9
(-0.5) 		285.2
(1.9) 		213.8
(-3) 		219.7
(-2.6) 		273.1
(1) 		301.9
(3) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189932_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11975
Sequence Type Exemplar sequence
Representative Public ID F25B3.1 NCBI
Target Description F25B3.1 /REP_DB=WormBase Gene ID /WP=CE05711 /TR=Q18749 /GB=CAA94758.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=actin binding domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073067(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073067 NCBI Caenorhabditis elegans F25B3.1 (F25B3.1) mRNA, complete cds. 11/11 None
SNAP00000011930 ENSEMBL cdna:SNAP chromosome:CEL140:V:9553582:9558329:1 11/11 None
GENEFINDER00000011939 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9553649:9558329:1 11/11 None
F25B3.1 ENSEMBL cdna:known chromosome:CEL140:V:9553228:9558613:1 gene:F25B3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9553227-9558329(+) 100.0 100.0
Public Domain and Genome References
Gene Title actin binding domain
Ensembl F25B3.1
Entrez Gene 179343 Entrez gene
SwissProt Q18749 EMBL-EBI
WormBase CE05711 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.17641.1.S1_S_AT similar to tangerin cfa
CANINE_2:CFA.17675.1.S1_S_AT similar to tangerin cfa
U133_X3P:91703_3P_AT EH domain binding protein 1-like 1 hs
U133_X3P:HS.325852.0.A1_3P_S_AT EH domain binding protein 1-like 1 hs
U133_X3P:4881574C_3P_S_AT EH domain binding protein 1-like 1 hs
HG-U133A_2:221755_AT EH domain binding protein 1-like 1 hs
HG-U133_PLUS_2:221755_AT EH domain binding protein 1-like 1 hs
HG-U133A:221755_AT EH domain binding protein 1-like 1 hs
HG-U95E:73273_AT EH domain binding protein 1-like 1 hs
HG-U95E:89652_F_AT EH domain binding protein 1-like 1 hs
HG-U133A_2:91703_AT EH domain binding protein 1-like 1 hs
HG-U133_PLUS_2:91703_AT EH domain binding protein 1-like 1 hs
HG-U133A:91703_AT EH domain binding protein 1-like 1 hs
HG-U95E:91703_AT EH domain binding protein 1-like 1 hs
U133_X3P:HS2.213967.1.A1_3P_AT EH domain binding protein 1-like 1 hs
HG-U133_PLUS_2:1557749_AT EH domain binding protein 1-like 1 hs
HG-U133_PLUS_2:1557228_AT EH domain binding protein 1-like 1 hs
MG-U74BV2:162592_AT tangerin mm
MOE430A:1448467_A_AT tangerin mm
MOUSE430A_2:1448467_A_AT tangerin mm
MOUSE430_2:1448467_A_AT tangerin mm
MOE430A:1416804_AT tangerin mm
MOUSE430A_2:1416804_AT tangerin mm
MOUSE430_2:1416804_AT tangerin mm
MU19KSUBA:TC15773_AT Tangerin (LOC114601), mRNA mm
MU19KSUBA:TC15774_AT Tangerin (LOC114601), mRNA mm
MU19KSUBA:TC22423_AT Tangerin (LOC114601), mRNA mm
RG-U34C:RC_AI105105_AT tangerin rn
RAE230A:1389071_AT tangerin rn
RAT230_2:1389071_AT tangerin rn
Protein Similarities
Method ID Description E-Value
blast CAA94758 Hypothetical protein F25B3.1 [Caenorhabditis elegans] emb|CAA94750.1| Hypothetical protein F25B3.1 [Caenorhabditis elegans] ref|NP_505468.1| F25B3.1 [Caenorhabditis elegans] 0.0
blast CAE72200 Hypothetical protein CBG19308 [Caenorhabditis briggsae] 0.0
blast AAH04660 LOC114601 protein [Mus musculus] gb|AAK32110.1| tangerin B [Mus musculus] 4.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.9E-20
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.9E-20
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.9E-20
Sequence
Target Sequence 
>C. ELEGANS:189932_S_AT
tcatcttcgcaagacgctctggcgacaccaaccaaaaagttctcttcccaatgggaaaaa
gacgtcgatgacgttgaaggaaccgccaatgagcttgttcgtattgacgaacgtatatcg
gatattacagcacaagccgatgttattcaagacaagatccgtgaaacagaagttggaagt
tcagaagaagaaatgttgactgcatcatatcttgagttgacaaatgaacggaacactctt
gtacatcgacaggaatactataatatcattgagacaattcgtcaggttacttcggaaatt
gaccaattgggaaaacaaatcaatgaagttcctgatgatttccctcgttctgatgagaat
aagacggcaacggataaactgattgaacaatatagtgatgctatgaaaacaaaaagtaac
ttggttcaaaagttatttgcaactgaggacgaaatcgaagaagattcggatcgtttgaag
aaccttactttggatagggctactcgctttgttcgtggaatagatcagcctgtttcagca
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATCTTCGCAAGACGCTCTGGCGA 504 621 2146 Antisense
GCTCTGGCGACACCAACCAAAAAGT 517 297 2161 Antisense
AACCGCCAATGAGCTTGTTCGTATT 676 145 2226 Antisense
GACTGCATCATATCTTGAGTTGACA 490 375 2343 Antisense
GAACGGAACACTCTTGTACATCGAC 280 347 2371 Antisense
GACAATTCGTCAGGTTACTTCGGAA 424 363 2418 Antisense
ATGAAGTTCCTGATGATTTCCCTCG 242 45 2468 Antisense
GATGATTTCCCTCGTTCTGATGAGA 208 411 2479 Antisense
CTTACTTTGGATAGGGCTACTCGCT 410 221 2629 Antisense
GCTACTCGCTTTGTTCGTGGAATAG 335 303 2644 Antisense
GGAATAGATCAGCCTGTTTCAGCAT 571 533 2662 Antisense
Sequence Source Affymetrix Proprietary Database