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[189920_at]

Description F44C8.5 /REP_DB=WormBase Gene ID /WP=CE10386 /TR=O16358 /GB=AAB65892.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=zinc finger protein
Cluster 6grp_high1_10_10 > 0      6grp_total > 09      4grp_total > 29      6grp_cgc > 0      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 100.5
(2.3)
P 		105.7
(3)
P 		60.7
(-3)
A 		62.9
(-2.7)
A 		45
B 78.9
(1.4)
A 		61.3
(-3)
M 		73.1
(-0)
M 		85.2
(3)
A 		24
C 74.8
(-0.5)
A 		73.2
(-0.7)
A 		59.5
(-3)
A 		95.6
(3)
P 		36
avr 84.7
(3) 		80.1
(1.6) 		64.4
(-3) 		81.2
(2) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 116.7
(3)
P 		95.2
(0.2)
P 		86.3
(-0.9)
P 		93.7
(0.1)
P 		69.9
(-3)
M 		72.4
(-2.7)
A 		47
B 110.7
(3)
P 		85.7
(-0.4)
A 		67
(-3)
M 		78.2
(-1.5)
P 		82.5
(-0.9)
P 		69.6
(-2.6)
P 		44
C 93.6
(0.8)
P 		81.9
(-0.8)
A 		88.3
(0.1)
A 		110.7
(3)
P 		95.4
(1)
M 		64.7
(-3)
A 		46
avr 107
(3) 		87.6
(-0.1) 		80.5
(-1.2) 		94.2
(1) 		82.6
(-0.8) 		68.9
(-3) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 36.6
(3)
P 		22.9
(-0)
P 		15.9
(-1.5)
A 		9.2
(-3)
A 		27
B 25.2
(3)
A 		15.1
(-3)
M 		16.9
(-1.9)
M 		18.1
(-1.2)
A 		10
C 24.8
(3)
A 		21
(0.4)
A 		20.7
(0.2)
A 		16.1
(-3)
P 		9
avr 28.9
(3) 		19.7
(-0.8) 		17.8
(-1.6) 		14.5
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 21.5
(-1.6)
P 		33.9
(3)
P 		18.8
(-2.6)
P 		23.8
(-0.7)
P 		21.1
(-1.7)
M 		17.6
(-3)
A 		16
B 38.5
(3)
P 		28.5
(0.3)
A 		16.3
(-3)
M 		20.7
(-1.8)
P 		33.5
(1.6)
P 		25.9
(-0.4)
P 		22
C 34.7
(3)
P 		25
(-1.5)
A 		22.5
(-2.6)
A 		22.3
(-2.7)
P 		30.9
(1.2)
M 		21.7
(-3)
A 		13
avr 31.6
(3) 		29.1
(1.8) 		19.2
(-3) 		22.3
(-1.5) 		28.5
(1.5) 		21.7
(-1.8) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189920_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15730
Sequence Type Exemplar sequence
Representative Public ID F44C8.5 NCBI
Target Description F44C8.5 /REP_DB=WormBase Gene ID /WP=CE10386 /TR=O16358 /GB=AAB65892.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=zinc finger protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028752(11), NM_001028751(11), AY305840(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028752 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-128) (nhr-128) mRNA, complete cds. 11/11 None
NM_001028751 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-128) (nhr-128) mRNA, complete cds. 11/11 None
AY305840 NCBI Caenorhabditis elegans clone yk818d8 nuclear receptor NHR-128 mRNA, partial cds. 11/11 None
SNAP00000011142 ENSEMBL cdna:SNAP chromosome:CEL140:V:2219221:2224405:1 11/11 None
GENEFINDER00000011149 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2221907:2228797:1 11/11 None
F44C8.5b ENSEMBL cdna:known chromosome:CEL140:V:2221775:2225149:1 gene:F44C8.5 11/11 None
F44C8.5a ENSEMBL cdna:known chromosome:CEL140:V:2221893:2225143:1 gene:F44C8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2221774-2224405(+) 94.54 100.0
Public Domain and Genome References
Ensembl F44C8.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAQ62454 Nuclear hormone receptor family protein 128, isoform b [Caenorhabditis elegans] ref|NP_001023923.1| Nuclear Hormone Receptor family member (nhr-128) [Caenorhabditis elegans] 0.0
blast T31792 hypothetical protein F44C8.3 - Caenorhabditis elegans 0.0
blast AAK84533 Nuclear hormone receptor family protein 18 [Caenorhabditis elegans] ref|NP_503608.2| Nuclear Hormone Receptor family member (nhr-18) [Caenorhabditis elegans] sp|O16360|NHR18_CAEEL Nuclear hormone receptor family member nhr-18 0.0
blast AAD03690 nuclear receptor NHR-18 [Caenorhabditis elegans] pir||T43356 nuclear receptor NHR-18 - Caenorhabditis elegans (fragment) 0.0
blast AAB65893 Nuclear hormone receptor family protein 56, isoform a [Caenorhabditis elegans] ref|NP_503605.3| Nuclear Hormone Receptor family member (nhr-56) [Caenorhabditis elegans] 1.0E-164
blast AAG15152 nuclear receptor NHR-56 [Caenorhabditis elegans] 1.0E-164
blast AAR11985 nuclear receptor NHR-128 [Caenorhabditis elegans] 1.0E-123
blast AAQ62454 Nuclear hormone receptor family protein 128, isoform b [Caenorhabditis elegans] ref|NP_001023923.1| Nuclear Hormone Receptor family member (nhr-128) [Caenorhabditis elegans] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 3.1E-17
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 2.6E-17
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 0.0064
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 1.7E-4
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 1.2E-5
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 6.7E-5
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 3.1E-17
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 1.5E-5
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 3.4E-18
Sequence
Target Sequence 
>C. ELEGANS:189920_AT
tagtaaaggctgcctggatcccctggacaattttggagaagctttacgagacctctgatt
accaaaggaaaaatatttttagcaagactgtactgatgtgtggcaatgatacatgtatgg
acatgaacaactacgagctcgatttatcctggctcacggactactcgttggaccagctta
catactttttcactccaccagaagcgaacaaaaactatttaaaaggccttgaagccttaa
tcgagctgaatccaagctccattgaggtcaactacatgcttcttcaactctcactccaac
acgccgggaaaattctacttggcagtgcgcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAGTAAAGGCTGCCTGGATCCCCTG 468 653 812 Antisense
GGAGAAGCTTTACGAGACCTCTGAT 104 519 846 Antisense
GGACATGAACAACTACGAGCTCGAT 415 525 930 Antisense
CGAGCTCGATTTATCCTGGCTCACG 656 249 945 Antisense
GACTACTCGTTGGACCAGCTTACAT 655 371 970 Antisense
GGACCAGCTTACATACTTTTTCACT 154 523 981 Antisense
TTTTTCACTCCACCAGAAGCGAACA 139 675 997 Antisense
GGCCTTGAAGCCTTAATCGAGCTGA 423 543 1036 Antisense
GCTGAATCCAAGCTCCATTGAGGTC 194 293 1056 Antisense
CTCACTCCAACACGCCGGGAAAATT 700 223 1101 Antisense
GAAAATTCTACTTGGCAGTGCGCAA 35 353 1119 Antisense
Sequence Source Affymetrix Proprietary Database