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[189917_at] zinc metalloprotease

Description F46C5.3 /REP_DB=WormBase Gene ID /WP=CE24986 /TR=Q20459 /GB=CAA91045.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_highest_10_10 > 33      6grp_high1_10_10 > 32      4grp_high_8_8 > 33      6grp_highest_nonorm > 80      6grp_total > 21      4grp_total > 54      6grp_highest_sub > 02      6grp_cgc > 62      6grp_high1_sub > 02_03      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 312
1 2 3 4 range
A 289.8
(3)
P 		127.9
(-0.7)
P 		105.7
(-1.2)
P 		24.3
(-3)
A 		266
B 247.1
(3)
P 		144.1
(-0.5)
P 		84.4
(-2.6)
P 		71.5
(-3)
M 		176
C 336.4
(3)
P 		122
(-2.1)
P 		130.4
(-1.9)
P 		85.5
(-3)
P 		251
avr 291.1
(3) 		131.3
(-1.2) 		106.8
(-1.8) 		60.4
(-3) 		231

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 292
1 2 3 4 5 6 range
A 149.6
(0.2)
P 		237.5
(2.9)
P 		241.4
(3)
P 		204
(1.9)
P 		148.4
(0.2)
P 		44.4
(-3)
P 		197
B 198.3
(-0.2)
P 		336.4
(3)
P 		331.5
(2.9)
P 		142.8
(-1.4)
P 		155.7
(-1.1)
P 		75
(-3)
P 		261
C 227.5
(2.2)
P 		252.2
(3)
P 		177.2
(0.5)
P 		215.3
(1.8)
P 		90.4
(-2.3)
P 		68.7
(-3)
P 		184
avr 191.8
(0.6) 		275.4
(3) 		250
(2.3) 		187.4
(0.5) 		131.5
(-1.1) 		62.7
(-3) 		213
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 105.5
(3)
P 		27.8
(-1.6)
P 		27.6
(-1.6)
P 		3.6
(-3)
A 		102
B 78.8
(3)
P 		35.6
(-1.1)
P 		19.6
(-2.6)
P 		15.2
(-3)
M 		64
C 111.4
(3)
P 		35.1
(-1.7)
P 		45.4
(-1.1)
P 		14.4
(-3)
P 		97
avr 98.6
(3) 		32.8
(-1.5) 		30.9
(-1.6) 		11.1
(-3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 27.6
(-1.6)
P 		84.5
(3)
P 		52.6
(0.4)
P 		51.7
(0.3)
P 		44.8
(-0.2)
P 		10.8
(-3)
P 		74
B 69
(-0.1)
P 		112
(3)
P 		80.5
(0.8)
P 		37.8
(-2.3)
P 		63.3
(-0.5)
P 		28
(-3)
P 		84
C 84.3
(3)
P 		76.9
(2.3)
P 		45.1
(-0.8)
P 		43.3
(-1)
P 		29.3
(-2.4)
P 		23
(-3)
P 		61
avr 60.3
(0.4) 		91.1
(3) 		59.4
(0.3) 		44.3
(-1) 		45.8
(-0.9) 		20.6
(-3) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189917_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2845
Sequence Type Exemplar sequence
Representative Public ID F46C5.3 NCBI
Target Description F46C5.3 /REP_DB=WormBase Gene ID /WP=CE24986 /TR=Q20459 /GB=CAA91045.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063479(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063479 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-25) (nas-25) mRNA, complete cds. 11/11 None
SNAP00000022655 ENSEMBL cdna:SNAP chromosome:CEL140:II:8826602:8828034:1 11/11 None
GENEFINDER00000022660 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8826024:8828034:1 11/11 None
F46C5.3 ENSEMBL cdna:known chromosome:CEL140:II:8825558:8828066:1 gene:F46C5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8826598-8828031(+) 100.0 100.0
Public Domain and Genome References
Gene Title zinc metalloprotease
Gene Symbol nas-25
Ensembl F46C5.3
Entrez Gene 174412 Entrez gene
SwissProt Q20459 EMBL-EBI
WormBase CE28935 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91045 Hypothetical protein F46C5.3 [Caenorhabditis elegans] ref|NP_495880.1| Nematode AStacin protease family member (nas-25) [Caenorhabditis elegans] sp|Q20459|NAS25_CAEEL Zinc metalloproteinase nas-25 precursor (Nematode astacin 25) 0.0
blast CAE59745 Hypothetical protein CBG03189 [Caenorhabditis briggsae] 0.0
blast CAA91045 Hypothetical protein F46C5.3 [Caenorhabditis elegans] ref|NP_495880.1| Nematode AStacin protease family member (nas-25) [Caenorhabditis elegans] sp|Q20459|NAS25_CAEEL Zinc metalloproteinase nas-25 precursor (Nematode astacin 25) 1.0E-180
blast CAE59745 Hypothetical protein CBG03189 [Caenorhabditis briggsae] 1.0E-169
blast CAE64915 Hypothetical protein CBG09735 [Caenorhabditis briggsae] 3.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 4.2E-62
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 1.9E-54
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495880.1 1 3-25
F46C5.3 1 3-25
GENEFINDER00000022660 1 3-25
Sequence
Target Sequence 
>C. ELEGANS:189917_AT
tattcagctgattctttttcgaacaaagatgacgaacaaacaatgcttccaaaagatcga
tcatttatcgaaacaatgggaagtatgattccaaacttttacgacttcgatcaaattaat
cagtattatcagtgttatgactcatgcagaaatgccgggcaattagctaattgtgcaaat
gggggaataccaaaccccaataattgtcaagtttgtaactgtccaatgggttatggtgga
gatctctgtgatcagaggccagaaggatgtggcagtacactcgttgcaacagatcgctgg
caaaaacagaagctttctgtgagattcagtagaaatgatgatcagtatttcacgttttgt
aattcatggattgtcggaccctccgatcgaacattgcaagttatttatgaaattacatcc
gattcaatcagacgacaaatttgttcatttggttgttatgaaggaggaattgaagtgaaa
catcttcaggacccaaggataacaaatgatagagattgttgtctgaatactccattaaat
ctaacaactactgttaatccactcccagttattctatatacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTCAGCTGATTCTTTTTCGAACA 206 663 424 Antisense
GATTCCAAACTTTTACGACTTCGAT 299 431 510 Antisense
GACTCATGCAGAAATGCCGGGCAAT 166 371 562 Antisense
AATGGGTTATGGTGGAGATCTCTGT 578 161 648 Antisense
ACACTCGTTGCAACAGATCGCTGGC 214 111 700 Antisense
GATGATCAGTATTTCACGTTTTGTA 214 411 760 Antisense
GTAATTCATGGATTGTCGGACCCTC 660 461 782 Antisense
GAAACATCTTCAGGACCCAAGGATA 155 357 900 Antisense
GATTGTTGTCTGAATACTCCATTAA 604 433 937 Antisense
ACAACTACTGTTAATCCACTCCCAG 2 113 967 Antisense
TCCACTCCCAGTTATTCTATATACG 320 589 981 Antisense
Sequence Source Affymetrix Proprietary Database