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[189906_at] zinc metalloprotease

Description T11F9.5 /REP_DB=WormBase Gene ID /WP=CE06417 /TR=Q22401 /GB=CAA98533.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_total > 60      4grp_total > 24      6grp_cgc > 94      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 16.7
(-3)
A 		29.3
(-0.1)
A 		42.8
(3)
A 		37
(1.7)
A 		26
B 39.4
(-1)
M 		50.7
(3)
A 		33.6
(-3)
A 		41.2
(-0.3)
A 		17
C 42.6
(3)
P 		24.2
(-3)
A 		35.6
(0.7)
P 		35.4
(0.7)
P 		18
avr 32.9
(-3) 		34.7
(-0.8) 		37.3
(2.3) 		37.9
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 22.8
(1.9)
A 		27.9
(3)
P 		18
(0.8)
A 		19.7
(1.2)
A 		0.8
(-3)
A 		17
(0.6)
A 		27
B 22.6
(-3)
A 		26
(-2)
A 		43.9
(3)
P 		42.6
(2.6)
P 		34.5
(0.4)
A 		25.3
(-2.2)
P 		21
C 35.1
(-1.2)
M 		28.9
(-2.2)
P 		37.1
(-0.9)
A 		63.1
(3)
A 		32.9
(-1.6)
M 		23.3
(-3)
P 		40
avr 26.8
(-1.5) 		27.6
(-1.3) 		33
(0.3) 		41.8
(3) 		22.7
(-2.8) 		21.9
(-3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 6.1
(-2.3)
A 		6.4
(-2)
A 		11.2
(3)
A 		5.4
(-3)
A 		6
B 12.6
(3)
M 		12.5
(2.9)
A 		7.8
(-3)
A 		8.8
(-1.8)
A 		5
C 14.1
(3)
P 		7
(-2.3)
A 		12.4
(1.7)
P 		6
(-3)
P 		8
avr 10.9
(3) 		8.6
(-0.3) 		10.5
(2.4) 		6.7
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 4.2
(-0.5)
A 		9.9
(3)
P 		3.9
(-0.7)
A 		5
(-0)
A 		0.2
(-3)
A 		4.1
(-0.6)
A 		10
B 7.9
(-3)
A 		8.7
(-2.2)
A 		10.7
(-0.2)
P 		11.3
(0.3)
P 		14
(3)
A 		9.4
(-1.5)
P 		6
C 13
(3)
M 		8.8
(-1.8)
P 		9.4
(-1.2)
A 		12.7
(2.7)
A 		10.6
(0.2)
M 		7.8
(-3)
P 		5
avr 8.4
(0) 		9.1
(1.6) 		8
(-0.9) 		9.7
(3) 		8.3
(-0.2) 		7.1
(-3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189906_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13457
Sequence Type Exemplar sequence
Representative Public ID T11F9.5 NCBI
Target Description T11F9.5 /REP_DB=WormBase Gene ID /WP=CE06417 /TR=Q22401 /GB=CAA98533.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073506(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073506 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-21) (nas-21) mRNA, complete cds. 11/11 None
T11F9.5 ENSEMBL cdna:known chromosome:CEL140:V:11475161:11476046:1 gene:T11F9.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000013920 7/11 Cross Hyb Matching Probes None
GENEFINDER00000013936 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11475160-11476046(+) 100.0 100.0
Public Domain and Genome References
Gene Title zinc metalloprotease
Gene Symbol nas-21
Ensembl T11F9.5
Entrez Gene 188422 Entrez gene
SwissProt Q22401 EMBL-EBI
WormBase CE06417 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98533 Hypothetical protein T11F9.5 [Caenorhabditis elegans] ref|NP_505907.1| Nematode AStacin protease family member (nas-21) [Caenorhabditis elegans] 1.0E-161
blast Q22401 Zinc metalloproteinase nas-21 precursor (Nematode astacin 21) 1.0E-142
blast CAE75254 Hypothetical protein CBG23214 [Caenorhabditis briggsae] 7.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 1.3E-22
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 9.6E-5
Sequence
Target Sequence 
>C. ELEGANS:189906_AT
attcgagagcaaccgtgctgagagcaatggaaaaaatatctaaccacacatgtatcaagt
tttctccaaaagatgcacgaataaaacttcgaattgtgagtgataaaggatgccaagcgg
ccattggacgagttgggggagatcagcaatatttgagttttcctacatcttgctatagtg
ttggaagtgcaagtgagctgattcatgtaattggctttcttcattctcatcaaagggctg
atcgtgacgaataccttaagttgaacttgcaaccatggagattgaatgattggtttcaaa
caatgcagtataaaaagtatttggatcaatggtggattgtaccatatgattatggtagta
tcatgcaataccatgattctgataatgaatatgggcctaaaaattcaaaatacttccgaa
caatgggttcgcaaataccttcgtatttcgattatctgatgattaacgaatactaccaat
gttcttgtgaaggggaagaacaaataaactgcaagaatcgagggtatccaaatccagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCGAGAGCAACCGTGCTGAGAGC 602 7 230 Antisense
ACCACACATGTATCAAGTTTTCTCC 321 85 272 Antisense
GATGCCAAGCGGCCATTGGACGAGT 605 415 338 Antisense
TTGGACGAGTTGGGGGAGATCAGCA 9 707 353 Antisense
TTGAGTTTTCCTACATCTTGCTATA 428 705 382 Antisense
ATGTAATTGGCTTTCTTCATTCTCA 149 49 434 Antisense
AAGGGCTGATCGTGACGAATACCTT 560 159 462 Antisense
ATGGGTTCGCAAATACCTTCGTATT 421 49 652 Antisense
AATACCTTCGTATTTCGATTATCTG 107 173 663 Antisense
AACGAATACTACCAATGTTCTTGTG 318 145 694 Antisense
AGAATCGAGGGTATCCAAATCCAGG 413 73 743 Antisense
Sequence Source Affymetrix Proprietary Database