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[189892_at]

Description F58B4.1 /REP_DB=WormBase Gene ID /WP=CE06011 /TR=Q20975 /GB=CAA98497.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 36      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 41      6grp_total > 07      4grp_total > 39      6grp_highest_sub > Cu      6grp_cgc > 24      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 597
1 2 3 4 range
A 986.7
(3)
P 		566.7
(-2)
P 		481.1
(-3)
P 		682.4
(-0.6)
P 		506
B 1051.9
(3)
P 		454.5
(-3)
P 		513.9
(-2.4)
P 		688.9
(-0.6)
P 		597
C 908.9
(3)
P 		611.1
(-1)
P 		460.7
(-3)
P 		719.9
(0.5)
P 		448
avr 982.5
(3) 		544.1
(-2.3) 		485.2
(-3) 		697.1
(-0.4) 		497

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 736
1 2 3 4 5 6 range
A 1109.4
(3)
P 		864.4
(0.3)
P 		757.5
(-0.8)
P 		559.4
(-3)
P 		558.3
(-3)
P 		693.1
(-1.5)
P 		551
B 1069.3
(3)
P 		799.9
(0.4)
P 		643.9
(-1.1)
P 		709.3
(-0.5)
P 		451.1
(-3)
P 		701.1
(-0.6)
P 		618
C 921.3
(2.8)
P 		943.2
(3)
P 		745.6
(0.9)
P 		671.5
(0.1)
P 		373.3
(-3)
P 		631.9
(-0.3)
P 		570
avr 1033.3
(3) 		869.2
(1.3) 		715.7
(-0.3) 		646.7
(-1.1) 		460.9
(-3) 		675.4
(-0.8) 		572
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 259
1 2 3 4 range
A 359.2
(3)
P 		123
(-2.5)
P 		125.8
(-2.4)
P 		99.9
(-3)
P 		259
B 335.4
(3)
P 		112.3
(-3)
P 		119.1
(-2.8)
P 		146.8
(-2.1)
P 		223
C 300.9
(3)
P 		175.7
(-1.2)
P 		160.6
(-1.7)
P 		121.4
(-3)
P 		180
avr 331.8
(3) 		137
(-2.6) 		135.2
(-2.6) 		122.7
(-3) 		209

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 251
1 2 3 4 5 6 range
A 204.5
(-0.7)
P 		307.4
(3)
P 		165
(-2.2)
P 		141.9
(-3)
P 		168.5
(-2)
P 		168.7
(-2)
P 		166
B 372.1
(3)
P 		266.3
(0.1)
P 		156.4
(-3)
P 		187.9
(-2.1)
P 		183.3
(-2.3)
P 		261.5
(-0.1)
P 		216
C 341.5
(3)
P 		287.8
(1.5)
P 		189.8
(-1.1)
P 		135
(-2.6)
P 		120.8
(-3)
P 		211.5
(-0.5)
P 		221
avr 306
(3) 		287.2
(2.3) 		170.4
(-2.4) 		154.9
(-3) 		157.5
(-2.9) 		213.9
(-0.7) 		151
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189892_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13585
Sequence Type Exemplar sequence
Representative Public ID F58B4.1 NCBI
Target Description F58B4.1 /REP_DB=WormBase Gene ID /WP=CE06011 /TR=Q20975 /GB=CAA98497.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=zinc metalloprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028823(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028823 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-31) (nas-31) mRNA, complete cds. 11/11 None
GENEFINDER00000018002 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10921469:10924343:-1 11/11 None
F58B4.1b ENSEMBL cdna:known chromosome:CEL140:V:10921462:10924423:-1 gene:F58B4.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028822 7/11 Cross Hyb Matching Probes None
SNAP00000017993 7/11 Cross Hyb Matching Probes None
F58B4.1a 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
186493 2 1 NM_001028823 189892_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10921528-10924343(-) 98.71 100.0
Public Domain and Genome References
Ensembl F58B4.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE48502 Hypothetical protein F58B4.1b [Caenorhabditis elegans] ref|NP_001023994.1| Nematode AStacin protease family member (nas-31) [Caenorhabditis elegans] sp|Q7JLI1|NAS31_CAEEL Zinc metalloproteinase nas-31 precursor (Nematode astacin 31) 0.0
blast CAA98497 Hypothetical protein F58B4.1a [Caenorhabditis elegans] ref|NP_001023993.1| Nematode AStacin protease family member (nas-31) [Caenorhabditis elegans] 0.0
blast CAE64915 Hypothetical protein CBG09735 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 6.4E-104
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.0E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.6E-9
Sequence
Target Sequence 
>C. ELEGANS:189892_AT
caggttacaggtactgctcttctgcagaccaaaacactgtgcatcgttcaactggctcac
tagttccaataattttattcaatcgatatgctagtacaaaagctgttcttgagtatcgtg
ctgtaactccatctgttgatgtctcagcaacatacacaacatttgcaccgattgttaaca
gttgccaggatttacatccgaactgcgacttttacaagtttttcggcatgtgtcgaagta
aaaaaattaggagtaattgtaaattcacatgtcacgattgtaataataataatgcatcgc
cttttggctctaactttttcaataataattacaatagtttcaataactggtacacgaata
aaaacaaaaattactacccctactcaaattcaaataacaataagccatggatttgtgccg
cgtatgcatggaatggtttttgtgttaatccgttctactcaatgcaagctcgtcattact
attgtgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGGTTACAGGTACTGCTCTTCTGC 120 209 1343 Antisense
AAACACTGTGCATCGTTCAACTGGC 370 129 1374 Antisense
GTTCAACTGGCTCACTAGTTCCAAT 134 445 1388 Antisense
GCTGTTCTTGAGTATCGTGCTGTAA 330 295 1444 Antisense
CGTGCTGTAACTCCATCTGTTGATG 106 245 1459 Antisense
GGATTTACATCCGAACTGCGACTTT 599 507 1530 Antisense
AATGCATCGCCTTTTGGCTCTAACT 449 165 1633 Antisense
TTACTACCCCTACTCAAATTCAAAT 220 661 1713 Antisense
TAAGCCATGGATTTGTGCCGCGTAT 707 637 1743 Antisense
AATCCGTTCTACTCAATGCAAGCTC 49 169 1789 Antisense
GCAAGCTCGTCATTACTATTGTGCA 344 321 1806 Antisense
Sequence Source Affymetrix Proprietary Database