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[189883_at] glucose transporter

Description F53H8.3 /REP_DB=WormBase Gene ID /WP=CE04656 /TR=Q20734 /GB=AAA82340.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=glucose transporter
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 97      4grp_highest_8_8 > 04      4grp_high_8_8 > 30      6grp_highest_nonorm > 83      6grp_total > 88      4grp_total > 34      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 338
1 2 3 4 range
A 462.7
(-3)
P 		704.4
(1.4)
P 		637.9
(0.2)
P 		790.9
(3)
P 		328
B 504.5
(-3)
P 		726.5
(1.8)
P 		647.2
(0.1)
P 		780.2
(3)
P 		276
C 453.2
(-3)
P 		721.5
(3)
P 		642.6
(1.2)
P 		546.6
(-0.9)
P 		268
avr 473.5
(-3) 		717.5
(3) 		642.6
(1.2) 		705.9
(2.7) 		244

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 541
1 2 3 4 5 6 range
A 421.1
(-2.4)
P 		431.7
(-2.1)
P 		404.4
(-2.8)
P 		395.8
(-3)
P 		633.9
(3)
P 		568.2
(1.3)
P 		238
B 346.5
(-3)
P 		466.6
(-1.2)
P 		440.8
(-1.6)
P 		476.5
(-1.1)
P 		540.2
(-0.2)
P 		756.6
(3)
P 		410
C 241.5
(-3)
P 		448.9
(-0.7)
P 		299.3
(-2.4)
P 		473.2
(-0.4)
P 		469.9
(-0.5)
P 		782.1
(3)
P 		541
avr 336.4
(-3) 		449.1
(-1.2) 		381.5
(-2.3) 		448.5
(-1.2) 		548
(0.5) 		702.3
(3) 		366
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 132
1 2 3 4 range
A 168.5
(3)
P 		152.9
(1.2)
P 		166.8
(2.8)
P 		115.8
(-3)
P 		53
B 160.9
(-0.8)
P 		179.4
(3)
P 		150
(-3)
P 		166.3
(0.3)
P 		29
C 150
(-0.4)
P 		207.4
(2.2)
P 		224
(3)
P 		92.2
(-3)
P 		132
avr 159.8
(0.8) 		179.9
(3) 		180.3
(3) 		124.8
(-3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 77.6
(-3)
P 		153.5
(1)
P 		88.1
(-2.4)
P 		100.4
(-1.8)
P 		191.3
(3)
P 		138.3
(0.2)
P 		114
B 120.6
(-2.5)
P 		155.3
(-1.3)
P 		107
(-3)
P 		126.2
(-2.3)
P 		219.5
(0.9)
P 		282.1
(3)
P 		175
C 89.5
(-2.6)
P 		137
(-1)
P 		76.2
(-3)
P 		95.2
(-2.4)
P 		152
(-0.5)
P 		261.8
(3)
P 		186
avr 95.9
(-2.8) 		148.6
(-0.5) 		90.4
(-3) 		107.3
(-2.3) 		187.6
(1.3) 		227.4
(3) 		137
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189883_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18454
Sequence Type Exemplar sequence
Representative Public ID F53H8.3 NCBI
Target Description F53H8.3 /REP_DB=WormBase Gene ID /WP=CE04656 /TR=Q20734 /GB=AAA82340.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=glucose transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075780(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075780 NCBI Caenorhabditis elegans F53H8.3 (F53H8.3) mRNA, complete cds. 11/11 None
GENEFINDER00000026709 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:938593:942545:1 11/11 None
F53H8.3 ENSEMBL cdna:known chromosome:CEL140:X:938513:942568:1 gene:F53H8.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026697 3/11 Cross Hyb Matching Probes None
SNAP00000026698 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:938592-942545(+) 100.0 100.0
Public Domain and Genome References
Gene Title glucose transporter
Ensembl F53H8.3
Entrez Gene 186195 Entrez gene
SwissProt Q20734 EMBL-EBI
WormBase CE04656 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
8643 carbohydrate transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
5351 sugar porter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA82340 Hypothetical protein F53H8.3 [Caenorhabditis elegans] ref|NP_508181.1| F53H8.3 [Caenorhabditis elegans] 0.0
blast CAE63596 Hypothetical protein CBG08087 [Caenorhabditis briggsae] 0.0
blast AAA82340 Hypothetical protein F53H8.3 [Caenorhabditis elegans] ref|NP_508181.1| F53H8.3 [Caenorhabditis elegans] 1.0E-148
blast CAE63596 Hypothetical protein CBG08087 [Caenorhabditis briggsae] 1.0E-139
blast XP_392938 PREDICTED: similar to CG1086-PB, isoform B [Apis mellifera] 2.0E-69
blast XP_392938 PREDICTED: similar to CG1086-PB, isoform B [Apis mellifera] 4.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 8.2E-35
Pfam IPR005828 EMBL-EBI General substrate transporter 1.4E-32
Pfam IPR005828 EMBL-EBI General substrate transporter 7.8E-74
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508181.1 12 12-34,54-76,89-106,116-138,151-170,180-202,323-345,360-382,389-411,424-446,459-481,486-508
F53H8.3 12 12-34,54-76,89-106,116-138,151-170,180-202,323-345,360-382,389-411,424-446,459-481,486-508
GENEFINDER00000026709 12 21-43,76-95,108-125,135-157,170-189,199-221,282-304,319-341,348-370,383-405,418-440,445-467
Sequence
Target Sequence 
>C. ELEGANS:189883_AT
gtacaagtccgttcctaattcacaaagttggaagacggtggctatcattgttccagttag
ccagttgtatggtggctttgatgttgctctctttattcacatttttgcagaattacgaaa
atatcgaatgggctagatacggaacaatattttctctggtgttctacatgtgtgtctatg
gtgttggttctccaatcccatggatcattgcaagtgaactttttacacagcagttccggg
caaccgctgtgacagtgtcggtatttgttgcgtggacatttgcgtttatcgttagcacat
cttatctaccattccagcagcttgttggaatcacattcagttatttccccttcatcatcg
ggcttgccgttttcggtatcttcatttatgtcctacttccggaaactcgtgatcgaccaa
tggttgaaattgtgacagaagttcatcaccgaacagcttccttgtcagctggaagaccat
gggatgctagtcggccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTACAAGTCCGTTCCTAATTCACAA 673 459 1139 Antisense
GACGGTGGCTATCATTGTTCCAGTT 289 377 1172 Antisense
ATGGTGGCTTTGATGTTGCTCTCTT 341 51 1207 Antisense
TATGGTGTTGGTTCTCCAATCCCAT 212 653 1315 Antisense
GGGCAACCGCTGTGACAGTGTCGGT 397 493 1376 Antisense
GTTGCGTGGACATTTGCGTTTATCG 113 441 1405 Antisense
TAGCACATCTTATCTACCATTCCAG 593 651 1431 Antisense
GAATCACATTCAGTTATTTCCCCTT 362 327 1466 Antisense
GCTTGCCGTTTTCGGTATCTTCATT 384 305 1500 Antisense
GTCCTACTTCCGGAAACTCGTGATC 683 469 1528 Antisense
AGACCATGGGATGCTAGTCGGCCAC 194 69 1612 Antisense
Sequence Source Affymetrix Proprietary Database