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[189865_s_at]

Description F36D4.3 /REP_DB=WormBase Gene ID /WP=CE26365 /CHR=5 /FEA=Sanger Annotation /DEF=locus:hum-2 myosin (ST.LOUIS) TR:Q20099 protein_id:AAA93485.1
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 82      4grp_highest_8_8 > 35      4grp_high_8_8 > 56      6grp_highest_nonorm > 65      6grp_total > 81      4grp_total > 63      6grp_highest_sub > 04      6grp_cgc > 86      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 320
1 2 3 4 range
A 804.1
(3)
P 		654.8
(-3)
P 		721.1
(-0.3)
P 		672.1
(-2.3)
P 		149
B 817.2
(3)
P 		626.3
(-3)
P 		778.5
(1.8)
P 		720
(-0.1)
P 		191
C 781.2
(0.8)
P 		578
(-3)
P 		897.5
(3)
P 		743.3
(0.1)
P 		320
avr 800.8
(3) 		619.7
(-3) 		799
(2.9) 		711.8
(0.1) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 752
1 2 3 4 5 6 range
A 852.5
(-3)
P 		1188
(2.8)
P 		903.1
(-2.1)
P 		1158.2
(2.3)
P 		1199.2
(3)
P 		1079.5
(0.9)
P 		347
B 835.7
(-3)
P 		898.1
(-2.4)
P 		1138.3
(-0.1)
P 		1469
(3)
P 		954.7
(-1.9)
P 		1022.2
(-1.2)
P 		633
C 831.4
(-3)
P 		1006.6
(-1.6)
P 		1061.9
(-1.2)
P 		1583.4
(3)
P 		1143.3
(-0.5)
P 		974.7
(-1.9)
P 		752
avr 839.9
(-3) 		1030.9
(-1) 		1034.4
(-0.9) 		1403.5
(3) 		1099.1
(-0.2) 		1025.5
(-1) 		564
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 292.8
(3)
P 		142.1
(-1.7)
P 		188.5
(-0.2)
P 		98.4
(-3)
P 		194
B 260.6
(3)
P 		154.7
(-2.9)
P 		180.4
(-1.5)
P 		153.4
(-3)
P 		107
C 258.6
(1.3)
P 		166.2
(-1.7)
P 		312.8
(3)
P 		125.3
(-3)
P 		188
avr 270.7
(3) 		154.3
(-1.8) 		227.2
(1.2) 		125.7
(-3) 		145

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 265
1 2 3 4 5 6 range
A 157.1
(-3)
P 		422.5
(3)
P 		196.7
(-2.1)
P 		293.7
(0.1)
P 		361.9
(1.6)
P 		262.8
(-0.6)
P 		265
B 290.8
(-2.2)
P 		298.9
(-1.8)
P 		276.4
(-3)
P 		389.1
(3)
P 		387.9
(2.9)
P 		381.2
(2.6)
P 		113
C 308.2
(-0.7)
P 		307.1
(-0.8)
P 		270.3
(-3)
P 		318.4
(-0.1)
P 		369.9
(3)
P 		326.3
(0.4)
P 		100
avr 252
(-2.8) 		342.8
(1.5) 		247.8
(-3) 		333.7
(1.1) 		373.2
(3) 		323.4
(0.6) 		125
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189865_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12141
Sequence Type Exemplar sequence
Representative Public ID F36D4.3 NCBI
Target Description F36D4.3 /REP_DB=WormBase Gene ID /WP=CE26365 /CHR=5 /FEA=Sanger Annotation /DEF=locus:hum-2 myosin (ST.LOUIS) TR:Q20099 protein_id:AAA93485.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073032(11), NM_001028715(11), NM_171505(11), NM_001028716(11), NM_001028717(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073032 NCBI Caenorhabditis elegans Heavy chain, Unconventional Myosin family member (hum-2) (hum-2) mRNA, complete cds. 11/11 A 
NM_001028715 NCBI Caenorhabditis elegans Heavy chain, Unconventional Myosin family member (hum-2) (hum-2) mRNA, complete cds. 11/11 None
NM_171505 NCBI Caenorhabditis elegans Heavy chain, Unconventional Myosin family member (hum-2) (hum-2) mRNA, complete cds. 11/11 None
NM_001028716 NCBI Caenorhabditis elegans Heavy chain, Unconventional Myosin family member (hum-2) (hum-2) mRNA, complete cds. 11/11 None
NM_001028717 NCBI Caenorhabditis elegans Heavy chain, Unconventional Myosin family member (hum-2) (hum-2) mRNA, complete cds. 11/11 None
SNAP00000027390 ENSEMBL cdna:SNAP chromosome:CEL140:V:9399112:9404344:1 11/11 None
GENEFINDER00000027398 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9399112:9402294:1 11/11 None
F36D4.3b ENSEMBL cdna:known chromosome:CEL140:V:9388738:9402646:1 gene:F36D4.3 11/11 A 
F36D4.3a ENSEMBL cdna:known chromosome:CEL140:V:9388738:9402647:1 gene:F36D4.3 11/11 A 
F36D4.3c.2 ENSEMBL cdna:known chromosome:CEL140:V:9399058:9402294:1 gene:F36D4.3 11/11 None
F36D4.3c.1 ENSEMBL cdna:known chromosome:CEL140:V:9399083:9402294:1 gene:F36D4.3 11/11 None
F36D4.3e ENSEMBL cdna:known chromosome:CEL140:V:9399406:9402294:1 gene:F36D4.3 11/11 None
F36D4.3f ENSEMBL cdna:known chromosome:CEL140:V:9400897:9402294:1 gene:F36D4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9388737-9402294(+) 100.0 100.0
Public Domain and Genome References
Ensembl F36D4.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR04675 Heavy chain, unconventional myosin protein 2, isoform a [Caenorhabditis elegans] ref|NP_505433.3| Heavy chain, Unconventional Myosin family member (hum-2) [Caenorhabditis elegans] 0.0
blast AAR04676 Heavy chain, unconventional myosin protein 2, isoform b [Caenorhabditis elegans] ref|NP_001023886.1| Heavy chain, Unconventional Myosin family member (hum-2) [Caenorhabditis elegans] gb|AAA97926.1| hum-2 [Caenorhabditis elegans] 0.0
blast CAE72166 Hypothetical protein CBG19269 [Caenorhabditis briggsae] 0.0
blast AAM29688 Heavy chain, unconventional myosin protein 2, isoform c [Caenorhabditis elegans] ref|NP_741590.1| Heavy chain, Unconventional Myosin family member (hum-2) [Caenorhabditis elegans] 0.0
blast AAO12438 Heavy chain, unconventional myosin protein 2, isoform e [Caenorhabditis elegans] ref|NP_001023887.1| Heavy chain, Unconventional Myosin family member (hum-2) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002710 EMBL-EBI Dilute (DIL) domain 1.2E-45
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Pfam IPR000048 EMBL-EBI IQ calmodulin-binding region 6.3E-8
Pfam IPR000048 EMBL-EBI IQ calmodulin-binding region 6.4E-8
Pfam IPR000048 EMBL-EBI IQ calmodulin-binding region 6.4E-9
Pfam IPR002710 EMBL-EBI Dilute (DIL) domain 1.2E-45
Pfam IPR002710 EMBL-EBI Dilute (DIL) domain 1.2E-45
Pfam IPR002710 EMBL-EBI Dilute (DIL) domain 1.2E-45
Pfam IPR002710 EMBL-EBI Dilute (DIL) domain 1.2E-45
Pfam IPR002710 EMBL-EBI Dilute (DIL) domain 1.2E-45
Pfam IPR007233 EMBL-EBI Sybindin-like protein 9.5E-125
Pfam IPR002710 EMBL-EBI Dilute (DIL) domain 1.2E-45
Sequence
Target Sequence 
>C. ELEGANS:189865_S_AT
atggccagatggatgtgtgctctcgccttgaactatatgatgttccgaagggagttgtgc
aattttgagaaggcaattcagatcaaacacaatgtgacacaaatccaaaattggctcaac
gcaaaaggactgtctgattgtcgtgatcacttcgaaccacttgtgcaagcatgtcatcta
ctgcaaagtcgcaaagatccttctaatctcgacacactatgtggtgaaatgacaagtcgg
ttgaagccacggcaagttgttgctatacttcaacactacgatccttctgatgaaatggag
gacggtctttctccagaattccttgtgcagattcagaagaaactgaacgagagagctatt
gcaaataatgatccaattgaggataaggacaaactgataatgctgggtacatatcttcca
ccattcgacactcaaccattctcttattctgatttcccacttgagactttaagcctccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCCAGATGGATGTGTGCTCTCG 310 53 4996 Antisense
GTGCTCTCGCCTTGAACTATATGAT 395 479 5012 Antisense
GGACTGTCTGATTGTCGTGATCACT 279 525 5122 Antisense
GTGATCACTTCGAACCACTTGTGCA 589 483 5138 Antisense
AAGATCCTTCTAATCTCGACACACT 615 155 5189 Antisense
GACAAGTCGGTTGAAGCCACGGCAA 633 361 5226 Antisense
TATACTTCAACACTACGATCCTTCT 34 661 5259 Antisense
CTTTCTCCAGAATTCCTTGTGCAGA 594 221 5302 Antisense
ATGCTGGGTACATATCTTCCACCAT 37 43 5395 Antisense
AACCATTCTCTTATTCTGATTTCCC 59 147 5429 Antisense
CCCACTTGAGACTTTAAGCCTCCCA 61 245 5451 Antisense
Sequence Source Affymetrix Proprietary Database