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[189848_at] DEAH-type helicase

Description F25H2.13 /REP_DB=WormBase Gene ID /WP=CE15894 /TR=Q93575 /GB=CAB02102.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=DEAH-type helicase
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_high_8_8 > 27      6grp_highest_nonorm > 83      6grp_total > 96      4grp_total > 36      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 395.8
(3)
P 		359.8
(0.8)
M 		299.3
(-3)
P 		325.6
(-1.4)
P 		97
B 350.2
(3)
P 		314.3
(-0.9)
P 		295.5
(-3)
P 		296.5
(-2.9)
P 		55
C 278
(-3)
P 		370.1
(1.6)
P 		312.2
(-1.3)
P 		398.8
(3)
P 		121
avr 341.3
(2.1) 		348.1
(3) 		302.3
(-3) 		340.3
(2) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 364
1 2 3 4 5 6 range
A 463.2
(1.2)
P 		314.7
(-3)
P 		339
(-2.3)
P 		348.7
(-2)
P 		510.3
(2.5)
P 		527.5
(3)
P 		213
B 398
(-2.1)
P 		362.5
(-2.9)
P 		359.1
(-3)
P 		437.1
(-1.1)
P 		571.8
(2.1)
P 		607.7
(3)
P 		249
C 243.4
(-3)
P 		403.2
(0.1)
P 		411.9
(0.3)
P 		552.7
(3)
P 		440.2
(0.8)
P 		527.5
(2.5)
P 		309
avr 368.2
(-2.7) 		360.1
(-3) 		370
(-2.7) 		446.2
(-0.3) 		507.4
(1.6) 		554.2
(3) 		194
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 144.1
(3)
P 		78.1
(-1.1)
M 		78.2
(-1.1)
P 		47.7
(-3)
P 		96
B 111.7
(3)
P 		77.6
(-1.2)
P 		68.5
(-2.3)
P 		63.2
(-3)
P 		49
C 92
(0.6)
P 		106.4
(2.7)
P 		108.8
(3)
P 		67.2
(-3)
P 		42
avr 115.9
(3) 		87.4
(-0) 		85.2
(-0.3) 		59.4
(-3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 85.4
(-2.1)
P 		111.9
(-0.1)
P 		73.8
(-3)
P 		88.4
(-1.9)
P 		154
(3)
P 		128.4
(1.1)
P 		80
B 138.5
(-0.9)
P 		120.7
(-1.6)
P 		87.2
(-3)
P 		115.8
(-1.8)
P 		232.3
(3)
P 		226.6
(2.8)
P 		145
C 90.2
(-3)
P 		123
(-0.7)
P 		104.8
(-2)
P 		111.1
(-1.5)
P 		142.4
(0.6)
P 		176.6
(3)
P 		86
avr 104.7
(-1.9) 		118.5
(-1) 		88.6
(-3) 		105.1
(-1.9) 		176.2
(2.9) 		177.2
(3) 		89
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189848_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1349
Sequence Type Exemplar sequence
Representative Public ID F25H2.13 NCBI
Target Description F25H2.13 /REP_DB=WormBase Gene ID /WP=CE15894 /TR=Q93575 /GB=CAB02102.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=DEAH-type helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060368(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060368 NCBI Caenorhabditis elegans F25H2.13 (F25H2.13) mRNA, complete cds. 11/11 None
F25H2.13 ENSEMBL cdna:known chromosome:CEL140:I:10580150:10585245:1 gene:F25H2.13 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000042627 4/11 Cross Hyb Matching Probes None
GENEFINDER00000042632 4/11 Cross Hyb Matching Probes None
SNAP00000028734 6/11 Cross Hyb Matching Probes None
GENEFINDER00000028736 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10580081-10585066(+) 100.0 100.0
Public Domain and Genome References
Gene Title DEAH-type helicase
Ensembl F25H2.13
Entrez Gene 172946 Entrez gene
SwissProt Q93575 EMBL-EBI
WormBase CE15894 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262066_AT helicase-related at
CANINE_2:CFAAFFX.20182.1.S1_AT similar to regulator of telomere elongation helicase 1 isoform 2 cfa
DROSOPHILA_2:1636909_AT dm
DROSGENOME1:144547_AT dm
CHICKEN:GGA.12530.1.S1_S_AT regulator of telomere elongation helicase 1 gga
CHICKEN:GGA.18194.1.S1_S_AT regulator of telomere elongation helicase 1 gga
CHICKEN:GGAAFFX.26754.1.S1_AT regulator of telomere elongation helicase 1 gga
CHICKEN:GGAAFFX.26754.2.S1_AT regulator of telomere elongation helicase 1 gga
HG-U133_PLUS_2:211526_S_AT regulator of telomere elongation helicase 1 hs
HG-U133A:211526_S_AT regulator of telomere elongation helicase 1 hs
HG-U133A_2:211526_S_AT regulator of telomere elongation helicase 1 hs
HG-U133_PLUS_2:216325_X_AT regulator of telomere elongation helicase 1 hs
HG-U133A_2:216325_X_AT regulator of telomere elongation helicase 1 hs
HG-U133A:216325_X_AT regulator of telomere elongation helicase 1 hs
HG-U133_PLUS_2:213829_X_AT regulator of telomere elongation helicase 1 hs
HG-U133A_2:213829_X_AT regulator of telomere elongation helicase 1 hs
HG-U133A:213829_X_AT regulator of telomere elongation helicase 1 hs
HG-U95AV2:35381_AT regulator of telomere elongation helicase 1 hs
HG-U133A:206467_X_AT regulator of telomere elongation helicase 1 hs
HG-FOCUS:206467_X_AT regulator of telomere elongation helicase 1 hs
HG-U133_PLUS_2:206467_X_AT regulator of telomere elongation helicase 1 hs
HG-U133A_2:206467_X_AT regulator of telomere elongation helicase 1 hs
HG-U133_PLUS_2:206092_X_AT regulator of telomere elongation helicase 1 hs
HG-U133A:206092_X_AT regulator of telomere elongation helicase 1 hs
HG-FOCUS:206092_X_AT regulator of telomere elongation helicase 1 hs
HG-U133A_2:206092_X_AT regulator of telomere elongation helicase 1 hs
HU35KSUBB:RC_AA025673_AT regulator of telomere elongation helicase 1 hs
U133_X3P:G7706540_3P_S_AT regulator of telomere elongation helicase 1 hs
U133_X3P:G4507584_3P_S_AT regulator of telomere elongation helicase 1 hs
U133_X3P:HS.281383.2.S1_3P_S_AT regulator of telomere elongation helicase 1 hs
HG-U95AV2:33727_R_AT regulator of telomere elongation helicase 1 hs
MOUSE430_2:1435516_X_AT regulator of telomere elongation helicase 1 mm
MOE430B:1435516_X_AT regulator of telomere elongation helicase 1 mm
MG-U74CV2:165546_AT regulator of telomere elongation helicase 1 mm
MOE430B:1436728_S_AT regulator of telomere elongation helicase 1 mm
MOUSE430_2:1436728_S_AT regulator of telomere elongation helicase 1 mm
MOE430B:1455347_AT regulator of telomere elongation helicase 1 mm
MOUSE430_2:1455347_AT regulator of telomere elongation helicase 1 mm
MOE430B:1456729_X_AT regulator of telomere elongation helicase 1 mm
MOUSE430_2:1456729_X_AT regulator of telomere elongation helicase 1 mm
MG-U74CV2:134220_AT DEAH helicase isoform 6 (Rtel) mRNA, complete cds; alternatively spliced mm
RG-U34C:RC_AI071280_AT similar to helicase-like protein NHL isoform 2 (predicted) rn
RG-U34C:RC_AI231057_AT similar to helicase-like protein NHL isoform 2 (predicted) rn
RAE230A:1374921_AT similar to helicase-like protein NHL isoform 2 (predicted) rn
RAT230_2:1374921_AT similar to helicase-like protein NHL isoform 2 (predicted) rn
RAE230B:1397955_AT similar to helicase-like protein NHL isoform 2 (predicted) rn
RAT230_2:1397955_AT similar to helicase-like protein NHL isoform 2 (predicted) rn
RAE230B:1391964_AT similar to helicase-like protein NHL isoform 2 (predicted) rn
RAT230_2:1391964_AT similar to helicase-like protein NHL isoform 2 (predicted) rn
RAE230A:1375711_AT Similar to helicase-like protein NHL isoform 2 (predicted) rn
RAT230_2:1375711_AT Similar to helicase-like protein NHL isoform 2 (predicted) rn
RG-U34B:RC_AI044876_AT Similar to helicase-like protein NHL isoform 2 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6139 nucleobase, nucleoside, nucleotide and nucleic acid metabolism inferred from electronic annotation QuickGO AmiGO
6289 nucleotide-excision repair inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
4003 ATP-dependent DNA helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
16818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02102 Hypothetical protein F25H2.13 [Caenorhabditis elegans] emb|CAB05720.1| Hypothetical protein F25H2.13 [Caenorhabditis elegans] ref|NP_492769.1| F25H2.13 [Caenorhabditis elegans] 0.0
blast CAE58983 Hypothetical protein CBG02256 [Caenorhabditis briggsae] 0.0
blast CAE56914 Hypothetical protein CBG24757 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010614 EMBL-EBI DEAD_2 5.2E-82
Sequence
Target Sequence 
>C. ELEGANS:189848_AT
agatatgttctcgcctgcaaatatgaaattggaaaagaaagaaatcactaaattacgtcc
tccacaattgataccttcaacttcttcagttttcagcctaccaacgaacgaagatgagct
gaaaatcaaaaaatgggagcaagaaaacgatattcaatgcttatcttcaataccccttga
atctaataaacgaaagtttaaaattgaaacaccagggccatcgacatctacgctgacaca
gaaaagtgaaccaccaaaaaagaagaaaattctgcttttgacacgaaatactctacctga
tgaatatcaaaaagcaattgaaattccaacttctgaacttttgaaagatatgtctgacga
caataaaaagcaatttgccaccacactcagaagctacaaagctgaaagtatcagatggga
tgaagtttttcaaagatttcggccaatttttgttccgcacaaggctgatttgtttattgc
ttgctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATATGTTCTCGCCTGCAAATATG 130 67 2442 Antisense
GAAATCACTAAATTACGTCCTCCAC 406 363 2482 Antisense
TTACGTCCTCCACAATTGATACCTT 678 679 2494 Antisense
GATACCTTCAACTTCTTCAGTTTTC 704 423 2511 Antisense
TTCAGTTTTCAGCCTACCAACGAAC 574 679 2526 Antisense
TCAATGCTTATCTTCAATACCCCTT 34 631 2595 Antisense
TTGAAACACCAGGGCCATCGACATC 40 701 2645 Antisense
CATCGACATCTACGCTGACACAGAA 40 213 2660 Antisense
TGCCACCACACTCAGAAGCTACAAA 390 587 2817 Antisense
TTTCGGCCAATTTTTGTTCCGCACA 25 671 2878 Antisense
GGCTGATTTGTTTATTGCTTGCTCC 637 539 2904 Antisense
Sequence Source Affymetrix Proprietary Database