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[189845_at] beta-galactosidase

Description T19B10.3 /REP_DB=WormBase Gene ID /WP=CE06457 /TR=Q27526 /GB=CAA98542.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=beta-galactosidase
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 32      4grp_high_8_8 > 43      6grp_highest_nonorm > 63      6grp_total > 64      4grp_total > 52      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 355
1 2 3 4 range
A 438.1
(3)
P 		431.4
(2.9)
P 		355.6
(1.2)
P 		165.5
(-3)
P 		273
B 479.4
(3)
P 		449.2
(2.1)
P 		350.3
(-0.6)
P 		266.4
(-3)
P 		213
C 430.3
(0.9)
P 		520.4
(3)
P 		441.7
(1.2)
P 		262.8
(-3)
P 		258
avr 449.3
(2.5) 		467
(3) 		382.5
(0.8) 		231.6
(-3) 		235

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 478
1 2 3 4 5 6 range
A 324.4
(-3)
P 		515.2
(-0.5)
P 		669
(1.5)
P 		782.8
(3)
P 		534.2
(-0.3)
P 		508
(-0.6)
P 		458
B 413.2
(-3)
P 		520.8
(-1.2)
P 		781.7
(3)
P 		650.2
(0.9)
P 		621.7
(0.4)
P 		657.6
(1)
P 		369
C 418.8
(-3)
P 		622.2
(0.2)
P 		673.7
(1)
P 		802
(3)
P 		434.6
(-2.8)
P 		700
(1.4)
P 		383
avr 385.5
(-3) 		552.7
(-0.2) 		708.1
(2.4) 		745
(3) 		530.2
(-0.6) 		621.9
(0.9) 		360
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 135
1 2 3 4 range
A 159.5
(3)
P 		93.7
(0.1)
P 		93
(0.1)
P 		24.2
(-3)
P 		135
B 152.9
(3)
P 		110.9
(0.4)
P 		81.2
(-1.5)
P 		56.8
(-3)
P 		96
C 142.5
(2.4)
P 		149.6
(2.8)
P 		153.9
(3)
P 		44.3
(-3)
P 		110
avr 151.6
(3) 		118.1
(1.2) 		109.4
(0.7) 		41.8
(-3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 193
1 2 3 4 5 6 range
A 59.8
(-3)
P 		183.2
(2.3)
P 		145.7
(0.7)
P 		198.5
(3)
P 		161.2
(1.4)
P 		123.7
(-0.2)
P 		139
B 143.8
(-3)
P 		173.4
(-1.4)
P 		189.8
(-0.5)
P 		172.2
(-1.4)
P 		252.6
(3)
P 		245.2
(2.6)
P 		109
C 155.2
(-2.1)
P 		189.8
(0.2)
P 		171.5
(-1)
P 		161.3
(-1.7)
P 		140.6
(-3)
P 		234.3
(3)
P 		94
avr 119.6
(-3) 		182.1
(1.6) 		169
(0.6) 		177.3
(1.2) 		184.8
(1.8) 		201.1
(3) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189845_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12818
Sequence Type Exemplar sequence
Representative Public ID T19B10.3 NCBI
Target Description T19B10.3 /REP_DB=WormBase Gene ID /WP=CE06457 /TR=Q27526 /GB=CAA98542.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=beta-galactosidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073448(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073448 NCBI Caenorhabditis elegans T19B10.3 (beta-galactosidase) mRNA, complete cds. 11/11 None
SNAP00000024743 ENSEMBL cdna:SNAP chromosome:CEL140:V:11225564:11228878:-1 11/11 None
GENEFINDER00000024758 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11225564:11231278:-1 11/11 None
T19B10.3 ENSEMBL cdna:known chromosome:CEL140:V:11225360:11228599:-1 gene:T19B10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11225563-11228878(-) 100.0 100.0
Public Domain and Genome References
Gene Title beta-galactosidase
Ensembl T19B10.3
Entrez Gene 188583 Entrez gene
SwissProt Q27526 EMBL-EBI
WormBase CE37992 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262351_AT glycosyl hydrolase family 35 protein at
CANINE:1582882_AT galactosidase, beta 1 cfa
CANINE_2:CFA.3737.1.S1_S_AT galactosidase, beta 1 cfa
CANINE_2:CFA.3737.2.S1_S_AT galactosidase, beta 1 cfa
CANINE:1582881_AT galactosidase, beta 1 cfa
DROSOPHILA_2:1633914_AT galactosidase dm
DROSGENOME1:143033_AT galactosidase dm
CHICKEN:GGAAFFX.11813.1.S1_AT galactosidase, beta 1 gga
CHICKEN:GGAAFFX.7597.1.S1_AT galactosidase, beta 1 gga
HUGENEFL:M34423_AT galactosidase, beta 1 hs
HG-U133A:201576_S_AT galactosidase, beta 1 hs
HG-U133_PLUS_2:201576_S_AT galactosidase, beta 1 hs
HG-FOCUS:201576_S_AT galactosidase, beta 1 hs
HG-U133A_2:201576_S_AT galactosidase, beta 1 hs
U133_X3P:G10834965_3P_A_AT galactosidase, beta 1 hs
HG-U95AV2:37742_AT galactosidase, beta 1 hs
HG-U133_PLUS_2:241344_AT Galactosidase, beta 1 hs
HG-U133B:241344_AT Galactosidase, beta 1 hs
HG-U95B:48001_AT Galactosidase, beta 1 hs
HG-U95D:86074_AT Galactosidase, beta 1 hs
HU35KSUBD:RC_N53976_AT Galactosidase, beta 1 hs
U133_X3P:HS.179864.0.S1_3P_AT Galactosidase, beta 1 hs
MOE430A:1416205_AT galactosidase, beta 1 mm
MOUSE430A_2:1416205_AT galactosidase, beta 1 mm
MOUSE430_2:1416205_AT galactosidase, beta 1 mm
MU11KSUBA:M57734_S_AT galactosidase, beta 1 mm
MG-U74AV2:103647_AT galactosidase, beta 1 mm
MG-U74BV2:110035_AT galactosidase, beta 1 mm
MG-U74AV2:161754_F_AT galactosidase, beta 1 mm
MOE430B:1435795_AT galactosidase, beta 1 mm
MOUSE430_2:1435795_AT galactosidase, beta 1 mm
MOE430A:1437159_AT Galactosidase, beta 1, mRNA (cDNA clone MGC:25403 IMAGE:4240285) mm
MOUSE430A_2:1437159_AT Galactosidase, beta 1, mRNA (cDNA clone MGC:25403 IMAGE:4240285) mm
MOUSE430_2:1437159_AT Galactosidase, beta 1, mRNA (cDNA clone MGC:25403 IMAGE:4240285) mm
RAE230A:1376781_AT Galactosidase, beta 1 rn
RAT230_2:1376781_AT Galactosidase, beta 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
9341 beta-galactosidase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4565 beta-galactosidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98542 Hypothetical protein T19B10.3 [Caenorhabditis elegans] ref|NP_505849.2| T19B10.3 [Caenorhabditis elegans] 0.0
blast CAE75193 Hypothetical protein CBG23139 [Caenorhabditis briggsae] 0.0
blast CAE65789 Hypothetical protein CBG10890 [Caenorhabditis briggsae] 1.0E-139
Protein Domains
Database ID Description E-Value
Pfam IPR001944 EMBL-EBI Glycoside hydrolase, family 35 2.0E-109
Pfam IPR001944 EMBL-EBI Glycoside hydrolase, family 35 3.9E-109
Pfam IPR001944 EMBL-EBI Glycoside hydrolase, family 35 2.0E-109
Sequence
Target Sequence 
>C. ELEGANS:189845_AT
acttgacaattccctcatttggagattttgttcatgtttatctcaatggaaagtacattg
acactctcacgcgaagatattacaacctgaccagaaactcagtgatcattgaaggttgcc
tggagaatgaagagaatcggctttttatgttggtcgaaaatcaaggaagaaaaacattcg
aaacaattaatgatagaaagggaattctttcggatgtttttatgaatggacaggcgattc
aattttggactcaatgtggaatcaagcttcctcttcaagaagacttctacttcagaaaag
caatgagaaacaattaccggaagaatgttaaaagtaatcaaaaacagggagttttcattg
gaattcttagtgtggatgccccaactgatacatggcttgacactactggatggggaaaag
gaattgcaattgtgaatggaagaaattttggaagatattggccaactaaaggtccacaaa
tgactttgtacattccagcagaattcttgaaaattggagaaaattcagtgatgatggttg
agctagaaggagctgaggaagcttgtacaagtacatcatcgtgcattgctgact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTGACAATTCCCTCATTTGGAGA 204 105 1628 Antisense
GAAAGTACATTGACACTCTCACGCG 237 361 1676 Antisense
GCGAAGATATTACAACCTGACCAGA 309 287 1698 Antisense
AGAGAATCGGCTTTTTATGTTGGTC 211 55 1758 Antisense
GGACAGGCGATTCAATTTTGGACTC 16 525 1855 Antisense
GTGGAATCAAGCTTCCTCTTCAAGA 419 491 1883 Antisense
GGAATTCTTAGTGTGGATGCCCCAA 102 533 1987 Antisense
GATGCCCCAACTGATACATGGCTTG 219 415 2002 Antisense
ATGGCTTGACACTACTGGATGGGGA 388 53 2019 Antisense
ATGACTTTGTACATTCCAGCAGAAT 509 45 2107 Antisense
AGTACATCATCGTGCATTGCTGACT 86 61 2197 Antisense
Sequence Source Affymetrix Proprietary Database