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[189841_s_at] sec7

Description K06H7.4 /REP_DB=WormBase Gene ID /WP=CE26942 /GEN=grp-1 /TR=SW:P34512 /GB=AAF99986.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=sec7
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 56      4grp_highest_8_8 > 53      4grp_high_8_8 > 08      6grp_highest_nonorm > 82      6grp_total > 57      4grp_total > 31      6grp_highest_sub > Cu      6grp_cgc > 37      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 267
1 2 3 4 range
A 368.6
(3)
P 		140.6
(-3)
P 		185.3
(-1.8)
P 		332.6
(2.1)
P 		228
B 389.8
(3)
P 		180.1
(-2.8)
P 		174
(-3)
P 		246.5
(-1)
P 		216
C 407.3
(3)
P 		170.2
(-3)
P 		180
(-2.8)
P 		315.6
(0.7)
P 		237
avr 388.6
(3) 		163.6
(-3) 		179.8
(-2.6) 		298.2
(0.6) 		225

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 285
(-3)
P 		350.3
(0.7)
P 		373.9
(2.1)
P 		318
(-1.1)
P 		295.5
(-2.4)
P 		389.8
(3)
P 		105
B 414.8
(3)
P 		306.2
(-3)
P 		324.9
(-2)
P 		307
(-3)
P 		329.4
(-1.7)
P 		408.8
(2.7)
P 		109
C 372.7
(1)
P 		344.9
(-0.1)
P 		318.1
(-1.1)
P 		334.3
(-0.5)
P 		267.6
(-3)
P 		425.8
(3)
P 		158
avr 357.5
(0.3) 		333.8
(-1) 		339
(-0.7) 		319.8
(-1.8) 		297.5
(-3) 		408.1
(3) 		111
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 134.2
(3)
P 		30.5
(-3)
P 		48.4
(-2)
P 		48.7
(-1.9)
P 		104
B 124.3
(3)
P 		44.5
(-2.7)
P 		40.3
(-3)
P 		52.5
(-2.1)
P 		84
C 134.8
(3)
P 		48.9
(-3)
P 		62.7
(-2)
P 		53.2
(-2.7)
P 		86
avr 131.1
(3) 		41.3
(-3) 		50.5
(-2.4) 		51.5
(-2.3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 52.5
(-3)
P 		124.6
(3)
P 		81.4
(-0.6)
P 		80.7
(-0.7)
P 		89.2
(0.1)
P 		94.9
(0.5)
P 		72
B 144.3
(2.3)
P 		101.9
(-1.1)
P 		78.9
(-3)
P 		81.3
(-2.8)
P 		133.8
(1.5)
P 		152.5
(3)
P 		74
C 138.2
(2.7)
P 		105.3
(0)
P 		81
(-1.9)
P 		67.2
(-3)
P 		86.6
(-1.5)
P 		142.5
(3)
P 		75
avr 111.7
(1) 		110.6
(0.8) 		80.4
(-2.6) 		76.4
(-3) 		103.2
(0) 		130
(3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189841_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6739
Sequence Type Exemplar sequence
Representative Public ID K06H7.4 NCBI
Target Description K06H7.4 /REP_DB=WormBase Gene ID /WP=CE26942 /GEN=grp-1 /TR=SW:P34512 /GB=AAF99986.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=sec7
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066363(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066363 NCBI Caenorhabditis elegans K06H7.4 (sec7) mRNA, complete cds. 11/11 None
SNAP00000036731 ENSEMBL cdna:SNAP chromosome:CEL140:III:8085771:8087980:1 11/11 None
GENEFINDER00000036743 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8085771:8087980:1 11/11 None
K06H7.4.1 ENSEMBL cdna:known chromosome:CEL140:III:8085625:8088354:1 gene:K06H7.4 11/11 None
K06H7.4.2 ENSEMBL cdna:known chromosome:CEL140:III:8085626:8088354:1 gene:K06H7.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8085768-8087978(+) 100.0 100.0
Public Domain and Genome References
Gene Title sec7
Gene Symbol grp-1
Ensembl K06H7.4
Entrez Gene 176140 Entrez gene
SwissProt P34512 EMBL-EBI
WormBase CE26942 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99986 Gtp exchange factor for arfs protein 1 [Caenorhabditis elegans] ref|NP_498764.1| K06H7.4 [Caenorhabditis elegans] sp|P34512|GRP1_CAEEL GTP exchange factor for ARFs 0.0
blast S44844 K06H7.4 protein - Caenorhabditis elegans 0.0
blast CAB38534 GRP1 protein [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000904 EMBL-EBI SEC7-like domain 1.0E-100
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.7E-29
Pfam IPR000904 EMBL-EBI SEC7-like domain 1.0E-100
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.7E-29
Sequence
Target Sequence 
>C. ELEGANS:189841_S_AT
ggtgcaattacaatcgaacagctcacagaagtatatgaatcagttagtgttacacaattc
aagatcccagatgaagtatcgacaagtggaaaaggaactgtgaacgatattctattacat
gctgaacgagaaggatggcttttcaagcagagtagtaatccattattttctggtgctctt
tcgtggaaaaaacgatggtttgttctttctgagaattgtctctactactttgaccaaatg
actgataaggaaccaaagggaatcatcacattggcaaatgttggaatacggaaggtcgag
gcaccctccaggccattcatgtttgagatattctctctatcggatggacaaatcaaagca
tgcaaaacagaacaagatgggcggcttgtcgaaggacgtcattcgatttacaaaatatgt
gcagtaaatgatgaagatatgagatcatggatcaacgcaatatcacgaatgatggcaccg
cagcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTGCAATTACAATCGAACAGCTCA 349 499 628 Antisense
GTGTTACACAATTCAAGATCCCAGA 612 485 674 Antisense
TATTTTCTGGTGCTCTTTCGTGGAA 328 663 791 Antisense
AAACGATGGTTTGTTCTTTCTGAGA 174 125 817 Antisense
GAATTGTCTCTACTACTTTGACCAA 630 323 840 Antisense
GAATACGGAAGGTCGAGGCACCCTC 249 325 911 Antisense
TATTCTCTCTATCGGATGGACAAAT 28 663 956 Antisense
GATGGGCGGCTTGTCGAAGGACGTC 569 407 1003 Antisense
GAAGGACGTCATTCGATTTACAAAA 164 333 1018 Antisense
GATCATGGATCAACGCAATATCACG 655 419 1070 Antisense
TCACGAATGATGGCACCGCAGCAAC 369 625 1090 Antisense
Sequence Source Affymetrix Proprietary Database