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[189832_at] trehalase precursor

Description F15A2.2 /REP_DB=WormBase Gene ID /WP=CE05631 /TR=Q19472 /GB=CAA94130.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=trehalase precursor
Cluster 6grp_high1_10_10 > 42      4grp_high_8_8 > 59      6grp_total > 62      4grp_total > 60      6grp_cgc > 74      6grp_high1_sub > 02_03      6grp_cgc_sub > 02_03_04_05      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 149
1 2 3 4 range
A 114.2
(1.4)
P 		91.8
(-0)
A 		139.1
(3)
P 		44.9
(-3)
A 		94
B 137.5
(0.8)
P 		102.3
(-0.6)
P 		191.5
(3)
P 		43
(-3)
P 		149
C 110.6
(-0)
P 		120
(0.5)
P 		170.3
(3)
P 		51
(-3)
A 		119
avr 120.8
(0.7) 		104.7
(-0.1) 		167
(3) 		46.3
(-3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 49.1
(-3)
A 		172.6
(3)
P 		163
(2.5)
P 		153.6
(2.1)
P 		133
(1.1)
P 		96.6
(-0.7)
P 		124
B 77.2
(-2.8)
P 		157.6
(2.7)
P 		162.3
(3)
P 		110.2
(-0.5)
P 		109.7
(-0.6)
P 		73.5
(-3)
P 		89
C 91.6
(-1.8)
P 		144.8
(0.2)
P 		217.6
(3)
P 		147.4
(0.3)
P 		139.3
(0)
P 		59.9
(-3)
P 		158
avr 72.6
(-3) 		158.3
(1.7) 		181
(3) 		137.1
(0.6) 		127.3
(0) 		76.7
(-2.8) 		108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 41.6
(3)
P 		19.9
(-0.7)
A 		36.4
(2.1)
P 		6.6
(-3)
A 		35
B 43.9
(2.9)
P 		25.3
(-0.3)
P 		44.4
(3)
P 		9.2
(-3)
P 		35
C 36.6
(0.3)
P 		34.5
(0.1)
P 		59.4
(3)
P 		8.6
(-3)
A 		51
avr 40.7
(2.1) 		26.6
(-0.1) 		46.7
(3) 		8.1
(-3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 9
(-3)
A 		61.4
(3)
P 		35.5
(0)
P 		39
(0.4)
P 		40.1
(0.6)
P 		23.5
(-1.3)
P 		52
B 26.9
(-3)
P 		52.5
(3)
P 		39.4
(-0.1)
P 		29.2
(-2.5)
P 		44.6
(1.1)
P 		27.4
(-2.9)
P 		26
C 33.9
(-0.7)
P 		44.2
(1.1)
P 		55.4
(3)
P 		29.6
(-1.4)
P 		45.1
(1.2)
P 		20.1
(-3)
P 		35
avr 23.3
(-3) 		52.7
(3) 		43.4
(1.1) 		32.6
(-1.1) 		43.3
(1.1) 		23.7
(-2.9) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189832_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17646
Sequence Type Exemplar sequence
Representative Public ID F15A2.2 NCBI
Target Description F15A2.2 /REP_DB=WormBase Gene ID /WP=CE05631 /TR=Q19472 /GB=CAA94130.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=trehalase precursor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077848(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077848 NCBI Caenorhabditis elegans TREhalase family member (tre-4) (tre-4) mRNA, complete cds. 11/11 None
GENEFINDER00000028424 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:13472076:13475775:1 11/11 None
SNAP00000028416 ENSEMBL cdna:SNAP chromosome:CEL140:X:13473487:13475775:1 11/11 None
F15A2.2 ENSEMBL cdna:known chromosome:CEL140:X:13472076:13475851:1 gene:F15A2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:13472075-13475775(+) 98.7 100.0
Public Domain and Genome References
Gene Title trehalase precursor
Gene Symbol tre-4
Ensembl F15A2.2
Entrez Gene 184505 Entrez gene
SwissProt Q19472 EMBL-EBI
WormBase CE35484 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5991 trehalose metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4555 alpha,alpha-trehalase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94130 Hypothetical protein F15A2.2 [Caenorhabditis elegans] ref|NP_510249.2| TREhalase family member (tre-4) [Caenorhabditis elegans] emb|CAD54511.1| trehalase [Caenorhabditis elegans] 0.0
blast CAE63144 Hypothetical protein CBG07446 [Caenorhabditis briggsae] 0.0
blast CAE63144 Hypothetical protein CBG07446 [Caenorhabditis briggsae] 1.0E-160
blast BAB40812.1 trehalase [Artemia franciscana] 4.0E-97
Protein Domains
Database ID Description E-Value
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 2.5E-65
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 1.3E-10
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 2.2E-13
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 2.5E-17
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 9.3E-10
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 1.3E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510249.2 1 583-605
F15A2.2 1 583-605
GENEFINDER00000028424 1 583-605
SNAP00000028416 1 370-392
Sequence
Target Sequence 
>C. ELEGANS:189832_AT
gatggataagtttgccgatcgtgttgaagattatatgaaaaaatcgggagcattggagtt
tgtgggcggtattccatcttcgttacctgctcagtccacgcaacagtgggattttccaaa
tgtttgggctccaaatcaacattttgtaattcaaagctttatggcttgcaataacagttt
cctgcagcaagaagctaaaaaacaagctatggaatttattgaaactgtgtataacggaat
gtacaacccaatagcaggactagatggaggagtatgggaaaagtacgacgcgaggagcac
gaatggggctccaggagctggaggagagtacgtggttcaagaaggatttggttggacaaa
tggagccgttatggacctcatttggacacttagagactctcacaaaaaacagattagatc
tgaaaatttgaagcttgatcaaggccaacactttgcattggtgttaacagcttttatatt
ttgtattgccatagcttttattgtgattatcaaaggaattatcaaaggaaacactttaaa
tcaacgggatgatgaagaagccggtgcccgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGGATAAGTTTGCCGATCGTGTT 471 407 1314 Antisense
AGTTTGTGGGCGGTATTCCATCTTC 365 63 1370 Antisense
CCTGCTCAGTCCACGCAACAGTGGG 566 265 1399 Antisense
TATGGCTTGCAATAACAGTTTCCTG 114 655 1473 Antisense
AAGTACGACGCGAGGAGCACGAATG 161 157 1594 Antisense
AGCACGAATGGGGCTCCAGGAGCTG 392 77 1609 Antisense
GGAGCTGGAGGAGAGTACGTGGTTC 707 521 1627 Antisense
GAGCCGTTATGGACCTCATTTGGAC 55 385 1676 Antisense
CATTTGGACACTTAGAGACTCTCAC 596 213 1692 Antisense
TGATCAAGGCCAACACTTTGCATTG 362 563 1749 Antisense
GATGATGAAGAAGCCGGTGCCCGTC 317 409 1861 Antisense
Sequence Source Affymetrix Proprietary Database