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[189828_s_at] heat shock protein

Description C15H9.6 /REP_DB=WormBase Gene ID /WP=CE08177 /GEN=hsp-3 /GB=AAB52671.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=heat shock protein
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 17      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 04      6grp_total > 06      4grp_total > 49      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5156
1 2 3 4 range
A 5315.8
(-3)
P 		9502.5
(3)
P 		9331.9
(2.8)
P 		6660
(-1.1)
P 		4187
B 5361.6
(-2.7)
P 		9003.3
(1.5)
P 		10299.3
(3)
P 		5142.9
(-3)
P 		5156
C 5176.9
(-3)
P 		8741.1
(2.8)
P 		8843.7
(3)
P 		5906.7
(-1.8)
P 		3667
avr 5284.8
(-3) 		9082.3
(2.4) 		9491.6
(3) 		5903.2
(-2.1) 		4207

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5772
1 2 3 4 5 6 range
A 6928.6
(3)
P 		6739
(2.7)
P 		3280
(-2.4)
P 		4408.3
(-0.7)
P 		2872.5
(-3)
P 		4085.5
(-1.2)
P 		4056
B 5773.8
(3)
P 		4492.1
(0.1)
P 		4166.2
(-0.6)
P 		3387.5
(-2.3)
P 		3162.7
(-2.8)
P 		3079.5
(-3)
P 		2694
C 8481.2
(3)
P 		4084.3
(-1.6)
P 		5241.7
(-0.4)
P 		2709.4
(-3)
P 		4072.2
(-1.6)
P 		3240.2
(-2.4)
P 		5772
avr 7061.2
(3) 		5105.1
(-0.2) 		4229.3
(-1.6) 		3501.7
(-2.8) 		3369.1
(-3) 		3468.4
(-2.8) 		3692
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2107
1 2 3 4 range
A 1935.5
(0.9)
P 		2062.7
(1.5)
P 		2439.5
(3)
P 		974.9
(-3)
P 		1465
B 1709.8
(-0.1)
P 		2223.6
(2.2)
P 		2386.6
(3)
P 		1096
(-3)
P 		1291
C 1713.7
(-0.9)
P 		2513.1
(1.4)
P 		3082.3
(3)
P 		996
(-3)
P 		2086
avr 1786.3
(-0.2) 		2266.5
(1.6) 		2636.1
(3) 		1022.3
(-3) 		1614

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2599
1 2 3 4 5 6 range
A 1277.2
(-1)
P 		2396.7
(3)
P 		714.3
(-3)
P 		1118.1
(-1.6)
P 		866.8
(-2.5)
P 		994.7
(-2)
P 		1682
B 2009
(3)
P 		1495.2
(0.2)
P 		1011.7
(-2.4)
P 		897.2
(-3)
P 		1285
(-0.9)
P 		1148.3
(-1.6)
P 		1112
C 3143.6
(3)
P 		1246.2
(-1.4)
P 		1334.1
(-1.2)
P 		544.9
(-3)
P 		1317.7
(-1.2)
P 		1084.7
(-1.8)
P 		2599
avr 2143.3
(3) 		1712.7
(1) 		1020
(-2.2) 		853.4
(-3) 		1156.5
(-1.6) 		1075.9
(-2) 		1290
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189828_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17218
Sequence Type Exemplar sequence
Representative Public ID C15H9.6 NCBI
Target Description C15H9.6 /REP_DB=WormBase Gene ID /WP=CE08177 /GEN=hsp-3 /GB=AAB52671.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=heat shock protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076618(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076618 NCBI Caenorhabditis elegans Heat Shock Protein family member (hsp-3) (hsp-3) mRNA, complete cds. 11/11 None
SNAP00000042799 ENSEMBL cdna:SNAP chromosome:CEL140:X:6089349:6091721:-1 11/11 None
GENEFINDER00000042811 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6089349:6092868:-1 11/11 None
C15H9.6.2 ENSEMBL cdna:known chromosome:CEL140:X:6088991:6091759:-1 gene:C15H9.6 11/11 None
C15H9.6.1 ENSEMBL cdna:known chromosome:CEL140:X:6088991:6091743:-1 gene:C15H9.6 11/11 None
C15H9.6.4 ENSEMBL cdna:known chromosome:CEL140:X:6089003:6092082:-1 gene:C15H9.6 11/11 None
C15H9.6.3 ENSEMBL cdna:known chromosome:CEL140:X:6089003:6091745:-1 gene:C15H9.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6089348-6091721(-) 100.0 100.0
Public Domain and Genome References
Gene Title heat shock protein
Gene Symbol hsp-3
Ensembl C15H9.6
Entrez Gene 180880 Entrez gene
SwissProt P27420 EMBL-EBI
WormBase CE08177 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15172_S_AT luminal binding protein 1 (BiP-1) (BP1) at
ATH1-121501:245956_S_AT luminal binding protein 1 (BiP-1) (BP1) at
CANINE:1592312_AT similar to 78 kDa glucose-regulated protein precursor (GRP 78) (Immunoglobulin heavy chain binding protein) (BiP) (Endoplasmic reticulum lumenal Ca(2+) binding protein grp78) cfa
CANINE_2:CFA.20174.1.S1_AT similar to 78 kDa glucose-regulated protein precursor (GRP 78) (Immunoglobulin heavy chain binding protein) (BiP) (Endoplasmic reticulum lumenal Ca(2+) binding protein grp78) cfa
CANINE_2:CFAAFFX.30936.1.S1_S_AT similar to 78 kDa glucose-regulated protein precursor (GRP 78) (Immunoglobulin heavy chain binding protein) (BiP) (Endoplasmic reticulum lumenal Ca(2+) binding protein grp78) cfa
DROSGENOME1:143193_AT Heat shock protein cognate 3 dm
DROSOPHILA_2:1635423_S_AT Heat shock protein cognate 3 dm
DROSGENOME1:144895_F_AT Heat shock protein cognate 3 dm
CHICKEN:GGA.4219.1.S1_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) gga
HU35KSUBD:RC_AA433933_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
HUGENEFL:M19645_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
U133_X3P:HS.75410.4.A1_3P_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
U133_X3P:HS.75410.1.S1_3P_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
HG-U133A_2:211936_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
HG-U133_PLUS_2:211936_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
HG-U133A:211936_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
HG-U133_PLUS_2:230031_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
HG-U133B:230031_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
HG-U95AV2:36614_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
HG-U95B:47634_AT heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) hs
MOE430A:1416064_A_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MOUSE430A_2:1416064_A_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MOUSE430_2:1416064_A_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MOUSE430_2:1447824_X_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MOE430B:1447824_X_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MU11KSUBA:D78645_F_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MU11KSUBB:MSA.479.0_F_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MU11KSUBB:MSA.29510.0_F_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MG-U74AV2:101955_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MOE430A:1427464_S_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MOUSE430A_2:1427464_S_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MOUSE430_2:1427464_S_AT heat shock 70kD protein 5 (glucose-regulated protein) mm
MU19KSUBB:TC28908_AT Heat shock 70kD protein 5 (glucose-regulated protein) (Hspa5), mRNA mm
MU19KSUBB:TC28908_G_AT Heat shock 70kD protein 5 (glucose-regulated protein) (Hspa5), mRNA mm
MU19KSUBC:TC36148_F_AT Heat shock 70kD protein 5 (glucose-regulated protein) (Hspa5), mRNA mm
MU19KSUBC:TC36148_R_AT Heat shock 70kD protein 5 (glucose-regulated protein) (Hspa5), mRNA mm
MU19KSUBC:TC36149_AT Heat shock 70kD protein 5 (glucose-regulated protein) (Hspa5), mRNA mm
RT-U34:M14050_S_AT heat shock 70kD protein 5 rn
RG-U34A:M14050_S_AT heat shock 70kD protein 5 rn
RG-U34A:S63521_I_AT heat shock 70kD protein 5 rn
RG-U34A:S63521_R_AT heat shock 70kD protein 5 rn
RAE230A:1370283_AT heat shock 70kD protein 5 rn
RAT230_2:1370283_AT heat shock 70kD protein 5 rn
YEAST_2:1777504_AT ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
42221 response to chemical stimulus inferred from sequence similarity QuickGO AmiGO
9306 protein secretion inferred from sequence similarity QuickGO AmiGO
6950 response to stress inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5788 endoplasmic reticulum lumen inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16887 ATPase activity inferred from sequence similarity QuickGO AmiGO
5515 protein binding inferred from sequence similarity QuickGO AmiGO
5509 calcium ion binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52671 Heat shock protein protein 3 [Caenorhabditis elegans] ref|NP_509019.1| Heat Shock Protein family member (hsp-3) [Caenorhabditis elegans] sp|P27420|HSP7C_CAEEL Heat shock 70 kDa protein C precursor pir||T15513 heat shock 70K protein C precursor HSP70C - Caenorhabditis elegans 0.0
blast AAA28074 BiP, heat shock protein 0.0
blast P19208 Heat shock 70 kDa protein C precursor emb|CAE68866.1| Hypothetical protein CBG14829 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 1.0E-126
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000042811 1 121-143
Sequence
Target Sequence 
>C. ELEGANS:189828_S_AT
tcaccatccaggtcttcgaaggagaacgcccaatgaccaaggacaaccatcagctcggaa
agttcgacctcaccggactcccaccagcaccacgcggagttccacaaattgaggttactt
tcgagattgacgtcaacggaatcctccacgttactgccgaggataagggaaccggaaaca
agaacaagatcaccatcaccaacgaccagaacagactcagccccgaagacatcgagcgca
tgatcaacgatgccgagaagttcgctgaggatgacaagaaggtcaaggataaggctgagg
ctcgcaacgagcttgagtcttacgcttacaacctgaaaaaccagattgaagacaaagaga
agctcggaggaaagctcgacgaggatgataagaagactatcgaggaggccgtcgaggagg
ctatctcatggctcggatccaacgctgaagcatctgctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCACCATCCAGGTCTTCGAAGGAGA 613 625 1409 Antisense
GAACGCCCAATGACCAAGGACAACC 274 347 1432 Antisense
TCAGCTCGGAAAGTTCGACCTCACC 311 625 1458 Antisense
TTGACGTCAACGGAATCCTCCACGT 645 703 1535 Antisense
TCCACGTTACTGCCGAGGATAAGGG 169 589 1553 Antisense
CACCAACGACCAGAACAGACTCAGC 390 201 1605 Antisense
GATAAGGCTGAGGCTCGCAACGAGC 170 423 1696 Antisense
GAGCTTGAGTCTTACGCTTACAACC 482 387 1717 Antisense
GGAGGCCGTCGAGGAGGCTATCTCA 437 515 1812 Antisense
CTATCTCATGGCTCGGATCCAACGC 544 223 1829 Antisense
GATCCAACGCTGAAGCATCTGCTGA 596 415 1844 Antisense
Sequence Source Affymetrix Proprietary Database