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[189825_at] RAS-related protein

Description C08F8.7 /REP_DB=WormBase Gene ID /WP=CE08025 /TR=O01312 /GB=CAA97427.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster 6grp_high1_10_10 > 82      6grp_total > 84      4grp_total > 41      6grp_cgc > 86      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 8.6
(-3)
A 		152.1
(2.5)
P 		165.3
(3)
P 		31.8
(-2.1)
A 		157
B 23.6
(-3)
A 		96.1
(1.7)
P 		116.8
(3)
P 		61.8
(-0.5)
P 		93
C 42.6
(-3)
M 		140
(3)
P 		139
(2.9)
P 		110.1
(1.2)
P 		97
avr 24.9
(-3) 		129.4
(2.4) 		140.4
(3) 		67.9
(-0.8) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 265
1 2 3 4 5 6 range
A 46.7
(-3)
A 		118.1
(-0.9)
P 		183.9
(1)
P 		254.6
(3)
P 		229.8
(2.3)
P 		151.6
(0)
P 		208
B 32.4
(-3)
P 		156.9
(-0.2)
P 		174.1
(0.2)
P 		297.1
(3)
P 		201
(0.8)
P 		143.2
(-0.5)
P 		265
C 41.6
(-3)
P 		136.6
(-0.5)
P 		93.3
(-1.6)
P 		267.3
(3)
P 		208.2
(1.4)
P 		190.7
(1)
P 		226
avr 40.2
(-3) 		137.2
(-0.5) 		150.4
(-0.2) 		273
(3) 		213
(1.5) 		161.8
(0.1) 		233
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 3.1
(-3)
A 		33
(1.5)
P 		43.2
(3)
P 		4.7
(-2.8)
A 		40
B 7.5
(-3)
A 		23.7
(2)
P 		27.1
(3)
P 		13.2
(-1.3)
P 		20
C 14.1
(-3)
M 		40.2
(1.6)
P 		48.4
(3)
P 		18.6
(-2.2)
P 		34
avr 8.2
(-3) 		32.3
(1.6) 		39.6
(3) 		12.2
(-2.2) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 8.6
(-3)
A 		42
(0.3)
P 		40
(0.1)
P 		64.6
(2.5)
P 		69.4
(3)
P 		36.9
(-0.2)
P 		61
B 11.3
(-3)
P 		52.2
(0.5)
P 		42.3
(-0.4)
P 		78.7
(2.7)
P 		81.7
(3)
P 		53.4
(0.6)
P 		70
C 15.4
(-3)
P 		41.7
(0)
P 		23.7
(-2)
P 		53.8
(1.4)
P 		67.4
(3)
P 		63.8
(2.6)
P 		52
avr 11.8
(-3) 		45.3
(0.3) 		35.3
(-0.7) 		65.7
(2.3) 		72.8
(3) 		51.4
(0.9) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189825_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10276
Sequence Type Exemplar sequence
Representative Public ID C08F8.7 NCBI
Target Description C08F8.7 /REP_DB=WormBase Gene ID /WP=CE08025 /TR=O01312 /GB=CAA97427.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069694(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069694 NCBI Caenorhabditis elegans RAP homolog (vertebrate Rap GTPase family) family member (rap-3) (rap-3) mRNA, complete cds. 11/11 None
SNAP00000012981 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11177258:11178050:1 11/11 None
GENEFINDER00000012989 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11177258:11178050:1 10/11 None
C08F8.7 ENSEMBL cdna:known chromosome:CEL140:IV:11177258:11177868:1 gene:C08F8.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11177263-11177874(+) 100.0 100.0
Public Domain and Genome References
Gene Title RAS-related protein
Gene Symbol rap-3
Ensembl C08F8.7
Entrez Gene 182417 Entrez gene
SwissProt O01312 EMBL-EBI
WormBase CE08025 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA97427 Hypothetical protein C08F8.7 [Caenorhabditis elegans] ref|NP_502095.1| RAP homolog (vertebrate Rap GTPase family) family member (rap-3) [Caenorhabditis elegans] 2.0E-91
blast CAA97427 Hypothetical protein C08F8.7 [Caenorhabditis elegans] ref|NP_502095.1| RAP homolog (vertebrate Rap GTPase family) family member (rap-3) [Caenorhabditis elegans] 8.0E-89
blast CAA97427 Hypothetical protein C08F8.7 [Caenorhabditis elegans] ref|NP_502095.1| RAP homolog (vertebrate Rap GTPase family) family member (rap-3) [Caenorhabditis elegans] 2.0E-78
blast CAE62016 Hypothetical protein CBG06025 [Caenorhabditis briggsae] 5.0E-75
blast CAE62016 Hypothetical protein CBG06025 [Caenorhabditis briggsae] 8.0E-75
blast CAE62016 Hypothetical protein CBG06025 [Caenorhabditis briggsae] 4.0E-73
blast CAA90983 Hypothetical protein C27B7.8 [Caenorhabditis elegans] ref|NP_501549.1| RAP homolog (vertebrate Rap GTPase family) family member (rap-1) [Caenorhabditis elegans] emb|CAE74068.1| Hypothetical protein CBG21720 [Caenorhabditis briggsae] 1.0E-43
blast CAA90983 Hypothetical protein C27B7.8 [Caenorhabditis elegans] ref|NP_501549.1| RAP homolog (vertebrate Rap GTPase family) family member (rap-1) [Caenorhabditis elegans] emb|CAE74068.1| Hypothetical protein CBG21720 [Caenorhabditis briggsae] 1.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.9E-26
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.4E-36
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.6E-41
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 4.2E-74
Sequence
Target Sequence 
>C. ELEGANS:189825_AT
gaggagttgggaaaagcgcattgactttgcaatacgtgcaaggaatatttgttcacactt
atgacgccacaattgaagattcctatagaaaattatcaaaagttgatgctgaaaatgcga
ggctggaaattttagatactgctggaacggtaaaactttcaattcaggaaattcaacata
tgttaattcaggagcaattcactggaatgcgagaaacatattaccgtactgctcagggat
ttgtactcgtcttttctcttgccgaaacttctacttttgaaaatttgaaacaaacgttta
cagatattatcgcaattcgtggaaaggaggttccaatgatattagtcggaaataaatctg
atttggcagaaacaaggcaagttgatgaatcagaagctcacaattttgctgaaaaactca
aaatcccatattttgagactagtgcaaggctcaaccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGAGTTGGGAAAAGCGCATTGAC 104 403 47 Antisense
GCGCATTGACTTTGCAATACGTGCA 159 279 62 Antisense
ATTTGTTCACACTTATGACGCCACA 16 19 93 Antisense
ATGACGCCACAATTGAAGATTCCTA 484 45 107 Antisense
TCAGGAGCAATTCACTGGAATGCGA 578 623 234 Antisense
AACATATTACCGTACTGCTCAGGGA 621 135 261 Antisense
GCTCAGGGATTTGTACTCGTCTTTT 706 297 277 Antisense
TTCTCTTGCCGAAACTTCTACTTTT 357 689 300 Antisense
ACAGATATTATCGCAATTCGTGGAA 33 109 346 Antisense
AAATCCCATATTTTGAGACTAGTGC 406 119 467 Antisense
TGAGACTAGTGCAAGGCTCAACCAG 642 565 480 Antisense
Sequence Source Affymetrix Proprietary Database