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[189824_at]

Description W03F8.5 /REP_DB=WormBase Gene ID /WP=CE17275 /GEN=lam-1 /TR=O44565 /GB=AAB94193.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=laminin
Cluster 6grp_total > 67      4grp_total > 20      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 6
(0.4)
A 		5.8
(-0.1)
A 		6.9
(3)
A 		4.8
(-3)
A 		2
B 6
(3)
A 		4.3
(0.4)
A 		2.1
(-3)
A 		2.9
(-1.8)
A 		4
C 2.6
(-3)
A 		4.7
(-1.6)
A 		2.8
(-2.9)
A 		11.5
(3)
A 		9
avr 4.9
(-0.6) 		4.9
(-0.6) 		3.9
(-3) 		6.4
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 5.8
(-2)
A 		2.7
(-2.8)
A 		1.7
(-3)
A 		3.1
(-2.7)
A 		2.4
(-2.8)
A 		27.3
(3)
A 		26
B 2.1
(-2.7)
A 		2.4
(-2)
A 		3
(-0.4)
A 		2
(-3)
A 		2.3
(-2.2)
A 		4.3
(3)
A 		2
C 2.6
(-1.4)
A 		3.7
(0.1)
A 		4.1
(0.7)
A 		2.9
(-1)
A 		1.4
(-3)
A 		5.8
(3)
A 		4
avr 3.5
(-2.1) 		2.9
(-2.5) 		2.9
(-2.5) 		2.7
(-2.6) 		2
(-3) 		12.5
(3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 2.2
(3)
A 		1.3
(-0.6)
A 		1.8
(1.4)
A 		0.7
(-3)
A 		2
B 1.9
(3)
A 		1.1
(-0.4)
A 		0.5
(-3)
A 		0.6
(-2.6)
A 		1
C 0.9
(-3)
A 		1.4
(-0)
A 		1
(-2.4)
A 		1.9
(3)
A 		1
avr 1.7
(3) 		1.3
(-1) 		1.1
(-3) 		1.1
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 1.1
(-2.3)
A 		1
(-2.4)
A 		0.4
(-3)
A 		0.8
(-2.6)
A 		0.7
(-2.7)
A 		6.7
(3)
A 		6
B 0.7
(-1.9)
A 		0.8
(-1.4)
A 		0.7
(-1.9)
A 		0.5
(-3)
A 		0.9
(-0.8)
A 		1.6
(3)
A 		1
C 1
(-0.6)
A 		1.1
(-0.2)
A 		1
(-0.6)
A 		0.6
(-2.2)
A 		0.4
(-3)
A 		1.9
(3)
A 		2
avr 0.9
(-2.4) 		1
(-2.1) 		0.7
(-2.8) 		0.6
(-3) 		0.7
(-2.8) 		3.4
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189824_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10416
Sequence Type Exemplar sequence
Representative Public ID W03F8.5 NCBI
Target Description W03F8.5 /REP_DB=WormBase Gene ID /WP=CE17275 /GEN=lam-1 /TR=O44565 /GB=AAB94193.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=laminin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028248(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028248 NCBI Caenorhabditis elegans W03F8.10 (W03F8.10) mRNA, complete cds. 11/11 None
SNAP00000036701 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5173322:5183208:1 11/11 None
GENEFINDER00000036711 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5173322:5183507:1 10/11 None
W03F8.10 ENSEMBL cdna:known chromosome:CEL140:IV:5180179:5183698:1 gene:W03F8.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5173327-5183214(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W03F8.10
Entrez Gene 3565510 Entrez gene
SwissProt Q7YZM5 EMBL-EBI
WormBase CE34385 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAP46270 Hypothetical protein W03F8.10 [Caenorhabditis elegans] ref|NP_001023419.1| W03F8.10 [Caenorhabditis elegans] 0.0
blast CAE72830 Hypothetical protein CBG20114 [Caenorhabditis briggsae] 0.0
blast AAP46271 Laminin related. see also lmb- protein 1 [Caenorhabditis elegans] ref|NP_500734.2| W03F8.5 [Caenorhabditis elegans] 0.0
blast CAE72754 Hypothetical protein CBG20003 [Caenorhabditis briggsae] 0.0
blast EAA01110 ENSANGP00000016875 [Anopheles gambiae str. PEST] ref|XP_321757.2| ENSANGP00000016875 [Anopheles gambiae str. PEST] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008211 EMBL-EBI Laminin, N-terminal 1.1E-122
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 9.6E-20
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 1.9E-19
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 3.4E-18
Pfam IPR008211 EMBL-EBI Laminin, N-terminal 4.4E-22
Pfam IPR008211 EMBL-EBI Laminin, N-terminal 9.4E-93
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 9.6E-20
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 1.9E-19
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 3.4E-18
Sequence
Target Sequence 
>C. ELEGANS:189824_AT
tcgcgaggcacttcaactggcagatcaagcctacaacaacgtaacttccgtacttgagga
gatcgacacaatgcaagtacaagcccgtgagctcatcgacaaggctcgtaactctacaga
agccgtcgaggggaaggcacaagctgccaacacgacacttgccgagttggaaggtgtgat
gagtggagtcaaggttgaatatctccagatttcggaatctgcaaagaatgcgttgactac
ggttgatgccgcgttggcagctgctacaaatgctgagcagaagaacaaacaaattcagac
cgatcttgagcgtgccacggagcttctcgaaaaacgaatggaaggaaatgttgctccaca
acagagagctgaaaagctaagagaaagagctgccaagctgctctatcaggctcagagaca
caacgatgatattgacaatttatcaaaggactcaaccgaaatgcgattggacgactacga
gacgattctggctgatttgaattcccgactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGCGAGGCACTTCAACTGGCAGAT 398 597 4872 Antisense
AAGCCCGTGAGCTCATCGACAAGGC 656 147 4952 Antisense
GCTCGTAACTCTACAGAAGCCGTCG 396 297 4975 Antisense
GGAAGGCACAAGCTGCCAACACGAC 142 531 5003 Antisense
AACACGACACTTGCCGAGTTGGAAG 419 135 5020 Antisense
TGACTACGGTTGATGCCGCGTTGGC 654 565 5105 Antisense
CCGCGTTGGCAGCTGCTACAAATGC 135 263 5120 Antisense
AATTCAGACCGATCTTGAGCGTGCC 172 175 5163 Antisense
CCAAGCTGCTCTATCAGGCTCAGAG 244 277 5264 Antisense
GGACGACTACGAGACGATTCTGGCT 669 523 5340 Antisense
TCTGGCTGATTTGAATTCCCGACTG 291 607 5358 Antisense
Sequence Source Affymetrix Proprietary Database