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[189820_at] beta-mannosidase

Description C33G3.4 /REP_DB=WormBase Gene ID /WP=CE08560 /TR=SW:Q93324 /GB=CAB01737.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=beta-mannosidase
Cluster 6grp_high1_10_10 > 83      6grp_total > 84      4grp_total > 60      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 386
1 2 3 4 range
A 305.2
(1.7)
P 		205.8
(-1)
A 		354.5
(3)
P 		129.2
(-3)
A 		225
B 311
(1.8)
P 		305.1
(1.7)
M 		355.1
(3)
P 		125.8
(-3)
A 		229
C 343.8
(0.3)
P 		285.7
(-0.7)
P 		512
(3)
P 		142.9
(-3)
A 		369
avr 320
(1.1) 		265.5
(-0.1) 		407.2
(3) 		132.6
(-3) 		275

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 555
1 2 3 4 5 6 range
A 140.2
(-3)
A 		404.9
(-0)
P 		379.2
(-0.3)
P 		673
(3)
P 		611
(2.3)
P 		483.9
(0.9)
P 		533
B 243.6
(-3)
P 		373.4
(-1.3)
P 		481.1
(0.2)
P 		695.4
(3)
P 		543
(1)
P 		469.9
(0)
P 		452
C 198
(-3)
P 		257.7
(-2)
P 		364.7
(-0.1)
P 		548.2
(3)
P 		444.3
(1.2)
P 		533.3
(2.7)
P 		350
avr 193.9
(-3) 		345.3
(-1) 		408.3
(-0.1) 		638.9
(3) 		532.8
(1.6) 		495.7
(1.1) 		445
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 111.1
(3)
P 		44.7
(-1.3)
A 		92.7
(1.8)
P 		18.9
(-3)
A 		92
B 99.2
(3)
P 		75.3
(1)
M 		82.3
(1.6)
P 		26.8
(-3)
A 		72
C 113.8
(0.5)
P 		82.2
(-0.7)
P 		178.4
(3)
P 		24.1
(-3)
A 		154
avr 108
(2.4) 		67.4
(-0.2) 		117.8
(3) 		23.3
(-3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 195
1 2 3 4 5 6 range
A 25.9
(-3)
A 		144
(1.5)
P 		82.6
(-0.9)
P 		170.7
(2.5)
P 		184.4
(3)
P 		117.8
(0.5)
P 		159
B 84.8
(-3)
P 		124.3
(-1.3)
P 		116.8
(-1.6)
P 		184.2
(1.4)
P 		220.6
(3)
P 		175.2
(1)
P 		136
C 73.4
(-3)
P 		78.6
(-2.7)
P 		92.8
(-1.9)
P 		110.2
(-0.9)
P 		143.8
(1)
P 		178.5
(3)
P 		105
avr 61.4
(-3) 		115.6
(-0.3) 		97.4
(-1.2) 		155
(1.6) 		182.9
(3) 		157.2
(1.7) 		122
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189820_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17682
Sequence Type Exemplar sequence
Representative Public ID C33G3.4 NCBI
Target Description C33G3.4 /REP_DB=WormBase Gene ID /WP=CE08560 /TR=SW:Q93324 /GB=CAB01737.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=beta-mannosidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077941(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077941 NCBI Caenorhabditis elegans C33G3.4 (beta-mannosidase) mRNA, complete cds. 11/11 None
SNAP00000041081 ENSEMBL cdna:SNAP chromosome:CEL140:X:14071119:14077163:1 11/11 None
GENEFINDER00000041087 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14071119:14076116:1 11/11 None
C33G3.4 ENSEMBL cdna:known chromosome:CEL140:X:14071105:14076206:1 gene:C33G3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14071118-14076116(+) 100.0 100.0
Public Domain and Genome References
Gene Title beta-mannosidase
Ensembl C33G3.4
Entrez Gene 181517 Entrez gene
SwissProt Q93324 EMBL-EBI
WormBase CE08560 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.16840.1.S1_AT similar to Beta-mannosidase precursor (Mannanase) (Mannase) cfa
DROSOPHILA_2:1624198_AT dm
DROSGENOME1:152253_AT dm
DROSGENOME1:149266_AT dm
CHICKEN:GGA.15982.1.S1_AT mannosidase, beta A, lysosomal gga
HG-U133A_2:203778_AT mannosidase, beta A, lysosomal hs
HG-U133A:203778_AT mannosidase, beta A, lysosomal hs
HG-U133_PLUS_2:203778_AT mannosidase, beta A, lysosomal hs
HG-FOCUS:203778_AT mannosidase, beta A, lysosomal hs
HU35KSUBB:RC_T83584_AT mannosidase, beta A, lysosomal hs
U133_X3P:G5174522_3P_AT mannosidase, beta A, lysosomal hs
HG-U95AV2:37472_AT mannosidase, beta A, lysosomal hs
MG-U74BV2:108097_AT mannosidase, beta A, lysosomal mm
MG-U74CV2:170018_R_AT mannosidase, beta A, lysosomal mm
MOE430A:1450626_AT mannosidase, beta A, lysosomal mm
MOUSE430A_2:1450626_AT mannosidase, beta A, lysosomal mm
MOUSE430_2:1450626_AT mannosidase, beta A, lysosomal mm
MG-U74CV2:169208_I_AT Mannosidase, beta A, lysosomal, mRNA (cDNA clone MGC:35624 IMAGE:4952710) mm
MU19KSUBB:TC34532_AT Mannosidase, beta A, lysosomal, mRNA (cDNA clone MGC:35624 IMAGE:4952710) mm
RG-U34B:RC_AA946434_AT mannosidase, beta A, lysosomal (predicted) rn
RAE230A:1371875_AT mannosidase, beta A, lysosomal (predicted) rn
RAT230_2:1371875_AT mannosidase, beta A, lysosomal (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4553 hydrolase activity, hydrolyzing O-glycosyl compounds inferred from electronic annotation QuickGO AmiGO
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01737 Hypothetical protein C33G3.4 [Caenorhabditis elegans] ref|NP_510342.1| C33G3.4 [Caenorhabditis elegans] sp|Q93324|MANBA_CAEEL Probable beta-mannosidase precursor (Mannanase) (Mannase) 0.0
blast CAE63039 Hypothetical protein CBG07306 [Caenorhabditis briggsae] 0.0
blast NP_956453 mannosidase, beta A, lysosomal [Danio rerio] gb|AAH45360.1| Mannosidase, beta A, lysosomal [Danio rerio] 1.0E-142
Sequence
Target Sequence 
>C. ELEGANS:189820_AT
gtagctgtgtattcgtttgctgatgaaaccgacttcaacttgaaagtattccttctcaac
gataatccatatttactacacaatattactgttaacgtacaaatgctcagctggggcaat
ggtcttgatccaattctcacaaatgagttccatattgatagtgtacctgcgggaagctcg
gaagtattaaaaactggaatcacattctccaaaatcacagaattatcggaatatttatac
gtgtcaacgctatatgattcatctggggtgaaaattcatgaagatgtactagttccggat
ttcttgtttgaggttgatttcaacacatttggtgatgtacaaataagtgatgttcaacga
attgacgaaaaaacttacgatctcaccattaccacggatagagtctcaccatttacttgg
ataacttgcaaaaaaccgttcaccggctggttcagtgacaatgggttccacatgattcag
aggctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAGCTGTGTATTCGTTTGCTGATG 426 457 2143 Antisense
AGTATTCCTTCTCAACGATAATCCA 502 61 2187 Antisense
TGCTCAGCTGGGGCAATGGTCTTGA 445 579 2246 Antisense
ATGGTCTTGATCCAATTCTCACAAA 270 51 2261 Antisense
GATAGTGTACCTGCGGGAAGCTCGG 274 425 2299 Antisense
GATGTACTAGTTCCGGATTTCTTGT 97 407 2425 Antisense
GAAAAAACTTACGATCTCACCATTA 639 353 2509 Antisense
CCATTACCACGGATAGAGTCTCACC 683 269 2528 Antisense
GAGTCTCACCATTTACTTGGATAAC 51 401 2543 Antisense
CGTTCACCGGCTGGTTCAGTGACAA 334 247 2579 Antisense
GGGTTCCACATGATTCAGAGGCTGC 174 497 2605 Antisense
Sequence Source Affymetrix Proprietary Database