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[189818_at]

Description K08F8.1 /REP_DB=WormBase Gene ID /WP=CE03467 /TR=Q21360 /GB=CAA91283.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ribosomal S6 kinase
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 67      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 49      6grp_total > 68      4grp_total > 01      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14648
1 2 3 4 range
A 5846.2
(-3)
P 		6264.9
(-2.8)
P 		6683
(-2.7)
P 		20293.4
(3)
P 		14447
B 5916.3
(-3)
P 		6183.6
(-2.9)
P 		7092.5
(-2.4)
P 		16832.7
(3)
P 		10916
C 5645.7
(-3)
P 		6085.4
(-2.8)
P 		6359.8
(-2.6)
P 		17202.5
(3)
P 		11557
avr 5802.7
(-3) 		6178
(-2.8) 		6711.8
(-2.6) 		18109.5
(3) 		12307

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6458
1 2 3 4 5 6 range
A 6152.6
(-0.4)
P 		3835.3
(-2.5)
P 		3682.1
(-2.7)
P 		3337.6
(-3)
P 		4884
(-1.6)
P 		9795.8
(3)
P 		6458
B 5155.3
(-1.2)
P 		3852.3
(-2.9)
P 		4279
(-2.3)
P 		3771
(-3)
P 		4529.3
(-2)
P 		8281.5
(3)
P 		4511
C 4429.1
(-1.8)
P 		3906.7
(-2.5)
P 		4095.3
(-2.3)
P 		3536.6
(-3)
P 		4792.9
(-1.3)
P 		8023.8
(3)
P 		4487
avr 5245.7
(-1) 		3864.8
(-2.6) 		4018.8
(-2.5) 		3548.4
(-3) 		4735.4
(-1.6) 		8700.4
(3) 		5152
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2227
1 2 3 4 range
A 2128.6
(-0.1)
P 		1359.9
(-3)
P 		1747.1
(-1.6)
P 		2970.6
(3)
P 		1611
B 1886.7
(-2)
P 		1527.2
(-3)
P 		1643.5
(-2.7)
P 		3587.1
(3)
P 		2060
C 1868.9
(-2.4)
P 		1749.6
(-3)
P 		2216.6
(-0.6)
P 		2900.6
(3)
P 		1151
avr 1961.4
(-1.4) 		1545.6
(-3) 		1869.1
(-1.8) 		3152.8
(3) 		1607

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2377
1 2 3 4 5 6 range
A 1134.2
(-1.7)
P 		1364
(-0.9)
P 		801.9
(-3)
P 		846.5
(-2.8)
P 		1473.8
(-0.5)
P 		2385
(3)
P 		1583
B 1793.8
(-0.7)
P 		1282.3
(-2.2)
P 		1039.1
(-2.9)
P 		998.8
(-3)
P 		1840.2
(-0.6)
P 		3088.1
(3)
P 		2089
C 1641.7
(-0.2)
P 		1192
(-1.5)
P 		1042.3
(-2)
P 		711.2
(-3)
P 		1550.9
(-0.4)
P 		2686
(3)
P 		1975
avr 1523.2
(-0.8) 		1279.4
(-1.6) 		961.1
(-2.7) 		852.2
(-3) 		1621.6
(-0.5) 		2719.7
(3) 		1868
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189818_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3962
Sequence Type Exemplar sequence
Representative Public ID K08F8.1 NCBI
Target Description K08F8.1 /REP_DB=WormBase Gene ID /WP=CE03467 /TR=Q21360 /GB=CAA91283.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ribosomal S6 kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063459(11), NM_001027068(10), NM_001027067(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063459 NCBI Caenorhabditis elegans K08F8.1a (K08F8.1) mRNA, complete cds. 11/11 None
NM_001027068 NCBI Caenorhabditis elegans K08F8.1d (K08F8.1) mRNA, complete cds. 10/11 None
NM_001027067 NCBI Caenorhabditis elegans K08F8.1c (K08F8.1) mRNA, complete cds. 11/11 None
SNAP00000029904 ENSEMBL cdna:SNAP chromosome:CEL140:II:8744050:8746991:1 11/11 None
GENEFINDER00000029916 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8744050:8746991:1 11/11 None
K08F8.1a.4 ENSEMBL cdna:known chromosome:CEL140:II:8743646:8747155:1 gene:K08F8.1 11/11 None
K08F8.1a.1 ENSEMBL cdna:known chromosome:CEL140:II:8743987:8747155:1 gene:K08F8.1 11/11 None
K08F8.1d.2 ENSEMBL cdna:known chromosome:CEL140:II:8743998:8747138:1 gene:K08F8.1 10/11 None
K08F8.1a.3 ENSEMBL cdna:known chromosome:CEL140:II:8743998:8747564:1 gene:K08F8.1 11/11 None
K08F8.1a.2 ENSEMBL cdna:known chromosome:CEL140:II:8743998:8747155:1 gene:K08F8.1 11/11 None
K08F8.1d.1 ENSEMBL cdna:known chromosome:CEL140:II:8743998:8747137:1 gene:K08F8.1 11/11 None
K08F8.1c ENSEMBL cdna:known chromosome:CEL140:II:8745422:8746991:1 gene:K08F8.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182125 7/11 Cross Hyb Matching Probes None
NM_001027069 4/11 Cross Hyb Matching Probes None
K08F8.1e.2 4/11 Cross Hyb Matching Probes None
K08F8.1e.4 4/11 Cross Hyb Matching Probes None
K08F8.1b.2 7/11 Cross Hyb Matching Probes None
K08F8.1b.1 3/11 Cross Hyb Matching Probes None
K08F8.1e.5 4/11 Cross Hyb Matching Probes None
K08F8.1e.1 4/11 Cross Hyb Matching Probes None
K08F8.1e.3 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174398 5 3 NM_001027067 189818_at (11)
NM_001027068 189818_at (10)
NM_063459 189818_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8744046-8746988(+) 100.0 100.0
Public Domain and Genome References
Ensembl K08F8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91283 Hypothetical protein K08F8.1a [Caenorhabditis elegans] ref|NP_495860.1| K08F8.1a [Caenorhabditis elegans] 0.0
blast CAE59719 Hypothetical protein CBG03152 [Caenorhabditis briggsae] 0.0
blast CAE48503 Hypothetical protein K08F8.1c [Caenorhabditis elegans] ref|NP_001022238.1| K08F8.1c [Caenorhabditis elegans] 0.0
blast CAH19101 Hypothetical protein K08F8.1d [Caenorhabditis elegans] ref|NP_001022239.1| K08F8.1d [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
hanks 2.3.1 CaMK Group; CaMK Other; MAPKAP2 2.0E-77
hanks 2.3.1 CaMK Group; CaMK Other; MAPKAP2 9.0E-81
hanks 2.3.1 CaMK Group; CaMK Other; MAPKAP2 3.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-61
Pfam IPR000719 EMBL-EBI Protein kinase 4.8E-52
Pfam IPR000719 EMBL-EBI Protein kinase 4.5E-57
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022238.1 1 169-188
K08F8.1c 1 169-188
Sequence
Target Sequence 
>C. ELEGANS:189818_AT
ttatgcggatatccgcccttctactcgatgaaaggtgtcgctttgtctccgggcatgcga
tcacgaattgccaatgcatattatgcgttcccacacgaggaatgggattgtgtgtcaaaa
gatactaaggatgacattcgttgtttgttgcgtacaaatccaagtgatcgtctcactatt
catgagctgatggcaacaccattggtaactggagaaccgattgtacctggaaaacacatc
acaacagctattgccattcctggagctgatgagtccgagtgtggaggaggagttttcgat
gacggatttattgttgaagaggaagaaactcctgatacggttgccgaaattcttccagtc
ccaaaaagtgtccgtttccttcgagacggtgtcaaggctccacgactccacagtatccaa
gaggaagttggtcgtgccatggaaattatgcgtatgggtactgatcaagtctacatcaag
aatccaactgcctcatgcaacaacttgttcgagagacgtcgcgctgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTATGCGGATATCCGCCCTTCTACT 648 679 1021 Antisense
GATGAAAGGTGTCGCTTTGTCTCCG 640 411 1047 Antisense
TCTCCGGGCATGCGATCACGAATTG 227 609 1066 Antisense
ATGCATATTATGCGTTCCCACACGA 616 43 1094 Antisense
GCTATTGCCATTCCTGGAGCTGATG 54 305 1267 Antisense
AGAAACTCCTGATACGGTTGCCGAA 570 67 1344 Antisense
TGTCCGTTTCCTTCGAGACGGTGTC 44 557 1389 Antisense
TCCACGACTCCACAGTATCCAAGAG 479 587 1419 Antisense
TACATCAAGAATCCAACTGCCTCAT 20 645 1492 Antisense
ACTGCCTCATGCAACAACTTGTTCG 352 99 1507 Antisense
ACTTGTTCGAGAGACGTCGCGCTGT 275 103 1523 Antisense
Sequence Source Affymetrix Proprietary Database