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[189810_at] cytochrome P450

Description ZK1320.4 /REP_DB=WormBase Gene ID /WP=CE01702 /TR=SW:Q09653 /GB=CAA87042.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 37      4grp_high_8_8 > 11      6grp_highest_nonorm > 40      6grp_total > 36      4grp_total > 13      6grp_highest_sub > Cu      6grp_cgc > 15      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 553
1 2 3 4 range
A 451.4
(3)
P 		202.3
(-2)
P 		155
(-3)
P 		356.3
(1.1)
P 		296
B 422.1
(0.4)
P 		242.2
(-2)
P 		167.4
(-3)
A 		616.9
(3)
P 		450
C 406.9
(-0.5)
P 		206.3
(-2.8)
P 		185.9
(-3)
P 		707.7
(3)
P 		522
avr 426.8
(1) 		216.9
(-2.3) 		169.4
(-3) 		560.3
(3) 		391

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1035
1 2 3 4 5 6 range
A 961.3
(3)
P 		579
(-0.3)
P 		534.4
(-0.7)
P 		506.6
(-0.9)
P 		262.8
(-3)
P 		749.8
(1.2)
P 		699
B 1221.7
(3)
P 		675.8
(-0.4)
P 		805.3
(0.4)
P 		490.9
(-1.6)
P 		270.1
(-3)
P 		746.5
(0)
P 		952
C 953.4
(3)
P 		812.3
(1.9)
P 		557.4
(-0.1)
P 		636.6
(0.5)
P 		187.2
(-3)
P 		829.4
(2)
P 		766
avr 1045.5
(3) 		689
(0.3) 		632.4
(-0.1) 		544.7
(-0.7) 		240
(-3) 		775.2
(1) 		806
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 164.3
(3)
P 		43.9
(-2.8)
P 		40.5
(-3)
P 		52.2
(-2.4)
P 		124
B 134.6
(3)
P 		59.8
(-1.7)
P 		38.8
(-3)
A 		131.5
(2.8)
P 		96
C 134.7
(3)
P 		59.3
(-3)
P 		64.8
(-2.6)
P 		119.3
(1.8)
P 		75
avr 144.5
(3) 		54.3
(-2.6) 		48
(-3) 		101
(0.3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 365
1 2 3 4 5 6 range
A 177.2
(1.6)
P 		205.9
(3)
P 		116.4
(-1.2)
P 		128.5
(-0.7)
P 		79.3
(-3)
P 		182.6
(1.9)
P 		127
B 425.1
(3)
P 		225
(-0.8)
P 		195.5
(-1.4)
P 		130
(-2.6)
P 		109.7
(-3)
P 		278.4
(0.2)
P 		315
C 353.4
(3)
P 		247.9
(0.8)
P 		141.9
(-1.3)
P 		128
(-1.6)
P 		60.6
(-3)
P 		277.6
(1.4)
P 		293
avr 318.6
(3) 		226.3
(0.6) 		151.3
(-1.3) 		128.8
(-1.8) 		83.2
(-3) 		246.2
(1.2) 		235
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189810_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3982
Sequence Type Exemplar sequence
Representative Public ID ZK1320.4 NCBI
Target Description ZK1320.4 /REP_DB=WormBase Gene ID /WP=CE01702 /TR=SW:Q09653 /GB=CAA87042.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cytochrome P450
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063684(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063684 NCBI Caenorhabditis elegans CYtochrome P450 family member (cyp-13A10) (cyp-13A10) mRNA, complete cds. 11/11 None
SNAP00000038582 ENSEMBL cdna:SNAP chromosome:CEL140:II:9660826:9662751:-1 11/11 None
GENEFINDER00000038603 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9660826:9665575:-1 11/11 None
ZK1320.4 ENSEMBL cdna:known chromosome:CEL140:II:9660761:9662857:-1 gene:ZK1320.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9660822-9663086(-) 100.0 100.0
Public Domain and Genome References
Gene Title cytochrome P450
Gene Symbol cyp-13A10
Ensembl ZK1320.4
Entrez Gene 174522 Entrez gene
SwissProt Q09653 EMBL-EBI
WormBase CE37736 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1626401_AT Cytochrome P450-6a2 dm
DROSGENOME1:143127_AT Cytochrome P450-6a2 dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4497 monooxygenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87042 Hypothetical protein ZK1320.4 [Caenorhabditis elegans] ref|NP_496085.3| CYtochrome P450 family member (cyp-13A10) [Caenorhabditis elegans] sp|Q09653|C13AA_CAEEL Putative cytochrome P450 CYP13A10 0.0
blast T27750 hypothetical protein ZK1320.4 - Caenorhabditis elegans 0.0
blast CAE57997 Hypothetical protein CBG01060 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
p450 CP11_MESAU Animalia; CYP1A1; ham; CP11_MESAU; Hamster; Q00557 1.0E-128
p450 CPA7_HUMAN Animalia; CYP2A7; hum; CPA7_HUMAN; Homo sapiens; P20853 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.3E-85
Pfam IPR001128 EMBL-EBI Cytochrome P450 3.3E-85
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496085.3 1 3-25
ZK1320.4 1 3-25
SNAP00000038582 1 3-25
GENEFINDER00000038603 8 5-24,45-67,77-99,120-142,178-195,226-248,263-280,406-423
Sequence
Target Sequence 
>C. ELEGANS:189810_AT
gaaatagtcggtcagtgtctcgtttttttgattgctggcttcgatacaactgctctatcc
ttatcctatactacatttctcctagcaacacatccagaagttcaaaagaaattacaagaa
gaaatagaacgagaatgcatagaaccatcaatttcatttgatcatctctcaaaactaaaa
tacatggattgtattatcaaagagactttacgtttgtaccctttgggaacaatggcgaac
tctcggagatgcatgagagccactaaattgggaaatgtagaagttgaagttggaacaatg
gttcaagtagatacttggagtcttcatactgatacaaagatttggggagatgatgcgaag
gagttcaagccagaaagatggcttgaccccaactgtgaccaagttttccaaaaaggagga
tatatttcattcggattaggacctcgtcagtgcgttggaatgcgattggcttatatggag
gaaaagatgctcttggctcatattcttaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATAGTCGGTCAGTGTCTCGTTT 163 363 1030 Antisense
GTGTCTCGTTTTTTTGATTGCTGGC 403 487 1044 Antisense
GATTGCTGGCTTCGATACAACTGCT 203 435 1059 Antisense
GATACAACTGCTCTATCCTTATCCT 615 423 1072 Antisense
ATCCTTATCCTATACTACATTTCTC 397 39 1086 Antisense
CTACATTTCTCCTAGCAACACATCC 139 227 1100 Antisense
ACAATGGCGAACTCTCGGAGATGCA 105 115 1258 Antisense
TGACCCCAACTGTGACCAAGTTTTC 629 225 1413 Antisense
ATTTCATTCGGATTAGGACCTCGTC 575 15 1453 Antisense
GGACCTCGTCAGTGCGTTGGAATGC 323 523 1468 Antisense
GATGCTCTTGGCTCATATTCTTAGA 683 413 1515 Antisense
Sequence Source Affymetrix Proprietary Database