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[189804_s_at] enoyl-CoA hydratase

Description F38H4.8 /REP_DB=WormBase Gene ID /WP=CE17808 /TR=Q20184 /GB=CAB01180.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=enoyl-CoA hydratase
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 63      4grp_highest_8_8 > 17      4grp_high_8_8 > 62      6grp_highest_nonorm > 74      6grp_total > 64      4grp_total > 58      6grp_cgc > 92      6grp_high1_sub > 03      6grp_cgc_sub > 03      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1054
1 2 3 4 range
A 811.1
(-0.3)
P 		966.5
(0.6)
P 		1356.3
(3)
P 		362.8
(-3)
P 		994
B 830.6
(-0.6)
P 		1110.2
(1.1)
P 		1417.1
(3)
P 		444.5
(-3)
P 		973
C 771.3
(0.1)
P 		1120.1
(2.9)
P 		1138.7
(3)
P 		366.2
(-3)
P 		773
avr 804.3
(-0.3) 		1065.6
(1.4) 		1304
(3) 		391.2
(-3) 		913

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 497
1 2 3 4 5 6 range
A 646.9
(-2)
P 		689.6
(-0.9)
P 		817.1
(2.7)
P 		829.8
(3)
P 		611.9
(-3)
P 		749.5
(0.8)
P 		218
B 506.8
(-3)
P 		702.3
(-0.6)
P 		1003.3
(3)
P 		739.7
(-0.2)
P 		580.2
(-2.1)
P 		688.9
(-0.8)
P 		497
C 509
(-3)
P 		625.2
(-1.2)
P 		903.5
(3)
P 		858.2
(2.3)
P 		615.4
(-1.4)
P 		810.9
(1.6)
P 		395
avr 554.2
(-3) 		672.4
(-1) 		908
(3) 		809.2
(1.3) 		602.5
(-2.2) 		749.8
(0.3) 		354
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 344
1 2 3 4 range
A 295.3
(1.8)
P 		209.8
(0.1)
P 		354.6
(3)
P 		53.1
(-3)
P 		302
B 264.9
(1.4)
P 		274.2
(1.6)
P 		328.4
(3)
P 		94.7
(-3)
P 		234
C 255.3
(0.5)
P 		322
(1.7)
P 		396.9
(3)
P 		61.7
(-3)
P 		335
avr 271.8
(1.2) 		268.7
(1.1) 		360
(3) 		69.8
(-3) 		290

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 119.3
(-3)
P 		245.2
(3)
P 		178
(-0.2)
P 		210.5
(1.3)
P 		184.6
(0.1)
P 		182.5
(0)
P 		126
B 176.3
(-3)
P 		233.8
(1.3)
P 		243.6
(2)
P 		195.9
(-1.5)
P 		235.7
(1.4)
P 		256.9
(3)
P 		81
C 188.7
(-2)
P 		190.8
(-1.9)
P 		230
(0.5)
P 		172.6
(-3)
P 		199.1
(-1.4)
P 		271.5
(3)
P 		99
avr 161.4
(-3) 		223.3
(1.9) 		217.2
(1.4) 		193
(-0.5) 		206.5
(0.6) 		237
(3) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189804_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9005
Sequence Type Exemplar sequence
Representative Public ID F38H4.8 NCBI
Target Description F38H4.8 /REP_DB=WormBase Gene ID /WP=CE17808 /TR=Q20184 /GB=CAB01180.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=enoyl-CoA hydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069845(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069845 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-2) (ech-2) mRNA, complete cds. 11/11 None
SNAP00000007682 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11855117:11856126:-1 11/11 None
GENEFINDER00000007686 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11855117:11857185:-1 11/11 None
F38H4.8 ENSEMBL cdna:known chromosome:CEL140:IV:11854949:11856311:-1 gene:F38H4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11855122-11856317(-) 100.0 100.0
Public Domain and Genome References
Gene Title enoyl-CoA hydratase
Gene Symbol ech-2
Ensembl F38H4.8
Entrez Gene 3564942 Entrez gene
SwissProt Q20184 EMBL-EBI
WormBase CE17808 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18702.1.S1_AT similar to enoyl Coenzyme A hydratase domain containing 3 cfa
CANINE_2:CFAAFFX.8236.1.S1_S_AT similar to enoyl Coenzyme A hydratase domain containing 3 cfa
DROSOPHILA_2:1636153_AT dm
DROSGENOME1:152860_AT dm
CHICKEN:GGA.6284.2.S1_A_AT enoyl Coenzyme A hydratase domain containing 3 gga
HG-U95B:47636_AT enoyl Coenzyme A hydratase domain containing 3 hs
HG-U133_PLUS_2:219298_AT enoyl Coenzyme A hydratase domain containing 3 hs
HG-U133A:219298_AT enoyl Coenzyme A hydratase domain containing 3 hs
HG-U133A_2:219298_AT enoyl Coenzyme A hydratase domain containing 3 hs
HU35KSUBD:RC_AA435985_AT enoyl Coenzyme A hydratase domain containing 3 hs
U133_X3P:G13375972_3P_AT enoyl Coenzyme A hydratase domain containing 3 hs
HG-U133_PLUS_2:239574_AT Enoyl Coenzyme A hydratase domain containing 3 hs
HG-U133B:239574_AT Enoyl Coenzyme A hydratase domain containing 3 hs
HG-U95B:52204_AT Enoyl Coenzyme A hydratase domain containing 3 hs
HU35KSUBA:RC_AA463737_AT Enoyl Coenzyme A hydratase domain containing 3 hs
U133_X3P:HS.22242.0.A1_3P_AT Enoyl Coenzyme A hydratase domain containing 3 hs
MOUSE430_2:1418862_AT enoyl Coenzyme A hydratase domain containing 3 mm
MOE430A:1418862_AT enoyl Coenzyme A hydratase domain containing 3 mm
MOUSE430A_2:1418862_AT enoyl Coenzyme A hydratase domain containing 3 mm
MG-U74BV2:116643_AT enoyl Coenzyme A hydratase domain containing 3 mm
MG-U74CV2:168381_S_AT enoyl Coenzyme A hydratase domain containing 3 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01180 Hypothetical protein F38H4.8 [Caenorhabditis elegans] ref|NP_502246.1| Enoyl-CoA Hydratase family member (ech-2) [Caenorhabditis elegans] 1.0E-154
blast CAB01180 Hypothetical protein F38H4.8 [Caenorhabditis elegans] ref|NP_502246.1| Enoyl-CoA Hydratase family member (ech-2) [Caenorhabditis elegans] 1.0E-151
blast CAE62134 Hypothetical protein CBG06178 [Caenorhabditis briggsae] 1.0E-141
blast CAE62134 Hypothetical protein CBG06178 [Caenorhabditis briggsae] 1.0E-137
blast CAB01180 Hypothetical protein F38H4.8 [Caenorhabditis elegans] ref|NP_502246.1| Enoyl-CoA Hydratase family member (ech-2) [Caenorhabditis elegans] 1.0E-126
blast CAE62134 Hypothetical protein CBG06178 [Caenorhabditis briggsae] 1.0E-117
blast AAI01898 Unknown (protein for MGC:124810) [Rattus norvegicus] 3.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 1.9E-29
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 1.7E-23
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 1.7E-23
Sequence
Target Sequence 
>C. ELEGANS:189804_S_AT
cctcctgtgatgtcgttgttgctggaaaaagttcgaaatttttagttccaggacagaagc
tcggattattctgctcgacacctggaatcgctcttgtgcgtgcagtaccacgtaaagtag
caatggacatgcttctgactgctcaaccaatcgactcggaagctgctcttcgaagtggac
ttgtctcccgcgtggttgaagacgatcaagtcaagttcgaagcgctcaatgtcgccgaac
aaattgggcacttcagccgctcagtgacggctcttggaaaagcgttcttctatacacaag
cagaattgagcactgtggatgcgtacagatatggttcccgagtgatggttggaaatctga
agctcaaggattgccaagaaggaatttctgcatttattggaaaacgacctgcagacttcg
agcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTCCTGTGATGTCGTTGTTGCTGG 516 267 404 Antisense
GAAGCTCGGATTATTCTGCTCGACA 406 343 459 Antisense
CACCTGGAATCGCTCTTGTGCGTGC 520 201 482 Antisense
AATGGACATGCTTCTGACTGCTCAA 224 163 525 Antisense
ACTGCTCAACCAATCGACTCGGAAG 351 95 541 Antisense
CTCTTCGAAGTGGACTTGTCTCCCG 56 231 569 Antisense
AGTTCGAAGCGCTCAATGTCGCCGA 79 63 617 Antisense
AACAAATTGGGCACTTCAGCCGCTC 363 135 641 Antisense
TCAGCCGCTCAGTGACGGCTCTTGG 479 625 656 Antisense
GATGCGTACAGATATGGTTCCCGAG 552 413 721 Antisense
AAAACGACCTGCAGACTTCGAGCAC 4 131 804 Antisense
Sequence Source Affymetrix Proprietary Database