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[189802_s_at] Glycerol kinase

Description R11F4.1 /REP_DB=WormBase Gene ID /WP=CE04828 /TR=SW:Q21944 /GB=AAA79749.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Glycerol kinase
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 59      6grp_total > 77      4grp_total > 10      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12270
1 2 3 4 range
A 1027.5
(-3)
P 		2641.1
(-2.2)
P 		2189.3
(-2.4)
P 		13297.5
(3)
P 		12270
B 1193
(-3)
P 		2347.4
(-2.4)
P 		1976.4
(-2.6)
P 		12007.5
(3)
P 		10815
C 1131.3
(-3)
P 		2252.5
(-2.4)
P 		1993.9
(-2.6)
P 		12762.1
(3)
P 		11631
avr 1117.3
(-3) 		2413.7
(-2.3) 		2053.2
(-2.5) 		12689
(3) 		11572

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5487
1 2 3 4 5 6 range
A 1904
(-3)
P 		2456.1
(-2.4)
P 		2614.2
(-2.2)
P 		2804.6
(-2)
P 		2416.8
(-2.4)
P 		7391
(3)
P 		5487
B 2023.6
(-3)
P 		2175.7
(-2.8)
P 		2130.3
(-2.8)
P 		2280.7
(-2.6)
P 		2766.2
(-1.8)
P 		5715.8
(3)
P 		3692
C 2051.4
(-3)
P 		2066.7
(-3)
P 		2784.1
(-2)
P 		2527.5
(-2.3)
P 		2974.6
(-1.7)
P 		6420
(3)
P 		4369
avr 1993
(-3) 		2232.8
(-2.7) 		2509.5
(-2.3) 		2537.6
(-2.3) 		2719.2
(-2) 		6508.9
(3) 		4516
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2185
1 2 3 4 range
A 374.1
(-3)
P 		573.3
(-2.2)
P 		572.3
(-2.2)
P 		1946.5
(3)
P 		1572
B 380.4
(-3)
P 		579.7
(-2.5)
P 		458
(-2.8)
P 		2558.8
(3)
P 		2178
C 374.5
(-3)
P 		647.6
(-2.1)
P 		694.9
(-1.9)
P 		2151.9
(3)
P 		1777
avr 376.3
(-3) 		600.2
(-2.3) 		575.1
(-2.4) 		2219.1
(3) 		1843

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1798
1 2 3 4 5 6 range
A 351
(-3)
P 		873.5
(-0.8)
P 		569.3
(-2.1)
P 		711.3
(-1.5)
P 		729.3
(-1.4)
P 		1799.5
(3)
P 		1449
B 704.1
(-2.3)
P 		724.2
(-2.2)
P 		517.3
(-3)
P 		604.1
(-2.7)
P 		1123.9
(-0.7)
P 		2131.4
(3)
P 		1614
C 760.4
(-2.1)
P 		630.6
(-2.6)
P 		708.6
(-2.3)
P 		508.3
(-3)
P 		962.5
(-1.3)
P 		2149.1
(3)
P 		1641
avr 605.2
(-3) 		742.8
(-2.4) 		598.4
(-3) 		607.9
(-3) 		938.6
(-1.6) 		2026.7
(3) 		1428
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189802_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5094
Sequence Type Exemplar sequence
Representative Public ID R11F4.1 NCBI
Target Description R11F4.1 /REP_DB=WormBase Gene ID /WP=CE04828 /TR=SW:Q21944 /GB=AAA79749.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Glycerol kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062320(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062320 NCBI Caenorhabditis elegans R11F4.1 (R11F4.1) mRNA, complete cds. 11/11 None
SNAP00000009533 ENSEMBL cdna:SNAP chromosome:CEL140:II:4092005:4094075:1 11/11 None
GENEFINDER00000009535 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4092005:4094075:1 11/11 None
R11F4.1.2 ENSEMBL cdna:known chromosome:CEL140:II:4091831:4094346:1 gene:R11F4.1 11/11 None
R11F4.1.1 ENSEMBL cdna:known chromosome:CEL140:II:4091924:4094346:1 gene:R11F4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4092003-4094074(+) 100.0 100.0
Public Domain and Genome References
Gene Title Glycerol kinase
Ensembl R11F4.1
Entrez Gene 173747 Entrez gene
SwissProt Q21944 EMBL-EBI
WormBase CE04828 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260274_AT glycerol kinase, putative at
CANINE:1591880_AT similar to glycerol kinase isoform 2 cfa
CANINE:1591969_S_AT similar to glycerol kinase isoform 2 cfa
CANINE:1591968_AT similar to glycerol kinase isoform 2 cfa
CANINE:1585148_AT similar to glycerol kinase isoform 2 cfa
CANINE_2:CFA.12018.1.A1_AT similar to glycerol kinase isoform 2 cfa
CANINE_2:CFA.19596.1.S1_AT similar to glycerol kinase isoform 2 cfa
CANINE_2:CFA.19596.1.S1_S_AT similar to glycerol kinase isoform 2 cfa
CANINE_2:CFAAFFX.21204.1.S1_S_AT similar to glycerol kinase isoform 2 cfa
DROSGENOME1:152740_AT dm
DROSOPHILA_2:1633956_S_AT dm
CHICKEN:GGA.11825.1.S1_AT glycerol kinase gga
CHICKEN:GGA.11825.1.S1_S_AT glycerol kinase gga
CHICKEN:GGAAFFX.24924.1.S1_AT glycerol kinase gga
HG-U133_PLUS_2:215966_X_AT glycerol kinase hs
HG-U133A_2:215966_X_AT glycerol kinase hs
HG-U133A:215966_X_AT glycerol kinase hs
HG-U133_PLUS_2:217167_X_AT glycerol kinase hs
HG-U133A_2:217167_X_AT glycerol kinase hs
HG-U133A:217167_X_AT glycerol kinase hs
HG-U133_PLUS_2:215977_X_AT glycerol kinase hs
HG-U133A_2:215977_X_AT glycerol kinase hs
HG-U133A:215977_X_AT glycerol kinase hs
HG-U133_PLUS_2:207387_S_AT glycerol kinase hs
HG-U133A_2:207387_S_AT glycerol kinase hs
HG-U133A:207387_S_AT glycerol kinase hs
HG-FOCUS:207387_S_AT glycerol kinase hs
HG-U95AV2:38130_S_AT glycerol kinase hs
HU35KSUBC:RC_N64351_AT glycerol kinase hs
HUGENEFL:X69886_S_AT glycerol kinase hs
HUGENEFL:X68285_S_AT glycerol kinase hs
HUGENEFL:L13943_AT glycerol kinase hs
U133_X3P:G4504006_3P_A_AT glycerol kinase hs
U133_X3P:HS.282272.0.S1_3P_S_AT glycerol kinase hs
U133_X3P:HS.1466.1.A1_3P_X_AT glycerol kinase hs
U133_X3P:HS.1466.3.S1_3P_X_AT glycerol kinase hs
U133_X3P:HS.1466.3.S1_3P_A_AT glycerol kinase hs
U133_X3P:HS.1466.2.A1_3P_AT glycerol kinase hs
HG-U133A_2:214681_AT glycerol kinase hs
HG-U133_PLUS_2:214681_AT glycerol kinase hs
HG-U133A:214681_AT glycerol kinase hs
HG-U95AV2:38129_AT glycerol kinase hs
HG-U95C:61873_AT glycerol kinase hs
HG-U95D:74732_S_AT glycerol kinase hs
HG-U95E:81410_AT glycerol kinase hs
HG-U133A_2:215975_X_AT glycerol kinase hs
HG-U133_PLUS_2:215975_X_AT glycerol kinase hs
HG-U133A:215975_X_AT glycerol kinase hs
MOUSE430_2:1445242_AT glycerol kinase mm
MOE430B:1445242_AT glycerol kinase mm
MU11KSUBA:AA445671_S_AT glycerol kinase mm
MU11KSUBA:U48403_S_AT glycerol kinase mm
MG-U74AV2:97525_AT glycerol kinase mm
MG-U74CV2:169383_R_AT glycerol kinase mm
MOE430A:1422703_AT glycerol kinase mm
MOUSE430A_2:1422703_AT glycerol kinase mm
MOUSE430_2:1422703_AT glycerol kinase mm
MOE430A:1422704_AT glycerol kinase mm
MOUSE430A_2:1422704_AT glycerol kinase mm
MOUSE430_2:1422704_AT glycerol kinase mm
MU19KSUBC:TC36226_I_AT Glycerol kinase, transcript variant 1, mRNA (cDNA clone MGC:5951 IMAGE:3593896) mm
YEAST_2:1769845_AT Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
6072 glycerol-3-phosphate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4370 glycerol kinase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA79749 Hypothetical protein R11F4.1 [Caenorhabditis elegans] ref|NP_494721.1| R11F4.1 [Caenorhabditis elegans] sp|Q21944|GLPK_CAEEL Probable glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase) (GK) 0.0
blast CAE71520 Hypothetical protein CBG18455 [Caenorhabditis briggsae] 0.0
blast AAT00543 putative glycerol kinase [Dictyocaulus viviparus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000577 EMBL-EBI Carbohydrate kinase, FGGY 9.0E-99
Pfam IPR000577 EMBL-EBI Carbohydrate kinase, FGGY 3.5E-46
Sequence
Target Sequence 
>C. ELEGANS:189802_S_AT
caatttggtgctgactctccagttgtctacgctctcgaaggatccgggtcgattggcggg
aatgttgtcagattcctcagagataatttcaagtttatctctgacgcgaaagaaatggaa
gggctttgcagaagtgtggaagacacctccggagcctactttgtcccatcattcaccgga
ttatacactccgtactgggactcgacagctcgaggaaccattctcggactcacccaggtc
acccaacgcgagcacatctgtctagctgctctgcgagccgttgctttccaaagtgctgag
atgattgctgcagttgaacaggatctcgaaggaggtaccaaggttacaactctgaaagtc
gatggaggaatgatcgctaacaagctgttcaatgagattcaagcggatattatgggacgg
gatattgtgactccaaagattaccgagatttccggatggggagcagcggttgccggtgga
atcggagctcagcagatcagtttggacgagtttttacagcagtctagtgaggataatcga
tacactccacaaaaagacgataactggaggtctgcagaacttgctcgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATTTGGTGCTGACTCTCCAGTTG 365 181 892 Antisense
TCCAGTTGTCTACGCTCTCGAAGGA 460 585 909 Antisense
GAAGGATCCGGGTCGATTGGCGGGA 370 333 928 Antisense
TGTCCCATCATTCACCGGATTATAC 631 555 1053 Antisense
TATACACTCCGTACTGGGACTCGAC 657 659 1073 Antisense
TCGAGGAACCATTCTCGGACTCACC 552 601 1101 Antisense
CAACGCGAGCACATCTGTCTAGCTG 357 187 1135 Antisense
AGCCGTTGCTTTCCAAAGTGCTGAG 559 83 1167 Antisense
GCGGTTGCCGGTGGAATCGGAGCTC 302 289 1357 Antisense
GAGCTCAGCAGATCAGTTTGGACGA 9 389 1376 Antisense
TGGAGGTCTGCAGAACTTGCTCGCT 258 549 1456 Antisense
Sequence Source Affymetrix Proprietary Database