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[189796_s_at] carbonic anhydrase

Description K05G3.3 /REP_DB=WormBase Gene ID /WP=CE04755 /TR=Q27504 /GB=AAB52498.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=carbonic anhydrase
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 42      4grp_high_8_8 > 40      6grp_highest_nonorm > 60      6grp_total > 27      4grp_total > 55      6grp_highest_sub > De      6grp_cgc > 04      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 957
1 2 3 4 range
A 1377.8
(3)
P 		565.5
(-2.1)
P 		712.2
(-1.2)
P 		421.1
(-3)
P 		957
B 1148.7
(3)
P 		540.2
(-2.2)
P 		561.4
(-2)
P 		450
(-3)
P 		699
C 1015.4
(3)
P 		653.6
(-1.1)
P 		699.3
(-0.6)
P 		489.7
(-3)
P 		526
avr 1180.6
(3) 		586.4
(-1.9) 		657.6
(-1.3) 		453.6
(-3) 		727

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 688
1 2 3 4 5 6 range
A 607.1
(3)
P 		578.4
(2.5)
P 		230.5
(-3)
P 		363.1
(-0.9)
P 		293.4
(-2)
P 		340
(-1.3)
P 		377
B 650.9
(3)
P 		457.2
(0.1)
P 		254.2
(-3)
P 		256.1
(-3)
P 		401.2
(-0.8)
P 		346.2
(-1.6)
P 		397
C 841.7
(3)
P 		424.6
(-0.6)
P 		318.2
(-1.6)
P 		153.4
(-3)
P 		361.1
(-1.2)
P 		412.6
(-0.7)
P 		688
avr 699.9
(3) 		486.7
(0.1) 		267.6
(-2.9) 		257.5
(-3) 		351.9
(-1.7) 		366.3
(-1.5) 		442
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 440
1 2 3 4 range
A 501.7
(3)
P 		122.8
(-2.2)
P 		186.2
(-1.3)
P 		61.6
(-3)
P 		440
B 366.3
(3)
P 		133.4
(-2.2)
P 		130.1
(-2.2)
P 		95.9
(-3)
P 		270
C 336.1
(3)
P 		187.9
(-0.5)
P 		243.7
(0.8)
P 		82.6
(-3)
P 		254
avr 401.4
(3) 		148
(-1.7) 		186.7
(-1) 		80
(-3) 		321

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 281
1 2 3 4 5 6 range
A 111.9
(-0.6)
P 		205.7
(3)
P 		50.2
(-3)
P 		92.1
(-1.4)
P 		88.5
(-1.5)
P 		82.8
(-1.7)
P 		156
B 226.5
(3)
P 		152.2
(0.3)
P 		61.7
(-3)
P 		67.8
(-2.8)
P 		163
(0.7)
P 		129.1
(-0.5)
P 		165
C 312
(3)
P 		129.6
(-0.9)
P 		81
(-1.9)
P 		30.8
(-3)
P 		116.8
(-1.2)
P 		138.1
(-0.7)
P 		281
avr 216.8
(3) 		162.5
(0.9) 		64.3
(-3) 		63.6
(-3) 		122.8
(-0.7) 		116.7
(-0.9) 		153
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189796_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17792
Sequence Type Exemplar sequence
Representative Public ID K05G3.3 NCBI
Target Description K05G3.3 /REP_DB=WormBase Gene ID /WP=CE04755 /TR=Q27504 /GB=AAB52498.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=carbonic anhydrase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078273(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078273 NCBI Caenorhabditis elegans Carbonic AnHydrase family member (cah-3) (cah-3) mRNA, complete cds. 11/11 None
GENEFINDER00000038240 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16486921:16497427:1 11/11 None
SNAP00000038236 ENSEMBL cdna:SNAP chromosome:CEL140:X:16487447:16489024:1 11/11 None
K05G3.3 ENSEMBL cdna:known chromosome:CEL140:X:16487427:16489298:1 gene:K05G3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16487446-16489024(+) 100.0 100.0
Public Domain and Genome References
Gene Title carbonic anhydrase
Gene Symbol cah-3
Ensembl K05G3.3
Entrez Gene 181713 Entrez gene
SwissProt Q27504 EMBL-EBI
WormBase CE04755 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1586208_AT similar to Carbonic anhydrase II (Carbonate dehydratase II) (CA-II) (Carbonic anhydrase C) cfa
CANINE_2:CFA.18716.1.S1_S_AT similar to Carbonic anhydrase II (Carbonate dehydratase II) (CA-II) (Carbonic anhydrase C) cfa
CANINE_2:CFA.2644.1.A1_AT similar to Carbonic anhydrase II (Carbonate dehydratase II) (CA-II) (Carbonic anhydrase C) cfa
CANINE_2:CFAAFFX.13723.1.S1_S_AT similar to Carbonic anhydrase II (Carbonate dehydratase II) (CA-II) (Carbonic anhydrase C) cfa
DROSOPHILA_2:1636374_AT Carbonic anhydrase 1 dm
DROSGENOME1:152261_AT Carbonic anhydrase 1 dm
CHICKEN:GGA.3986.2.S1_A_AT carbonic anhydrase II gga
HG-U133A:209301_AT carbonic anhydrase II hs
HG-U133_PLUS_2:209301_AT carbonic anhydrase II hs
HG-FOCUS:209301_AT carbonic anhydrase II hs
HG-U133A_2:209301_AT carbonic anhydrase II hs
HUGENEFL:Y00339_S_AT carbonic anhydrase II hs
U133_X3P:G179794_3P_AT carbonic anhydrase II hs
HG-U95AV2:40095_AT carbonic anhydrase II hs
HG-U95D:75200_R_AT carbonic anhydrase II hs
MOE430A:1448752_AT carbonic anhydrase 2 mm
MOUSE430A_2:1448752_AT carbonic anhydrase 2 mm
MOUSE430_2:1448752_AT carbonic anhydrase 2 mm
MU11KSUBA:K00811_S_AT carbonic anhydrase 2 mm
MG-U74AV2:92642_AT carbonic anhydrase 2 mm
MU19KSUBB:TC26629_AT Carbonic anhydrase 2 (Car2), mRNA mm
MU19KSUBB:TC26630_S_AT Carbonic anhydrase 2 (Car2), mRNA mm
RG-U34A:X58294_AT carbonic anhydrase 2 rn
RG-U34A:U60578CDS_S_AT carbonic anhydrase 2 rn
RAE230A:1386922_AT carbonic anhydrase 2 rn
RAT230_2:1386922_AT carbonic anhydrase 2 rn
RAE230A:1367733_AT carbonic anhydrase 2 rn
RAT230_2:1367733_AT carbonic anhydrase 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6730 one-carbon compound metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4089 carbonate dehydratase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52498 Carbonic anhydrase protein 3 [Caenorhabditis elegans] ref|NP_510674.1| Carbonic AnHydrase family member (cah-3) [Caenorhabditis elegans] sp|Q27504|CAH3_CAEEL Putative carbonic anhydrase 3 (Carbonate dehydratase 3) 1.0E-147
blast AAB52498 Carbonic anhydrase protein 3 [Caenorhabditis elegans] ref|NP_510674.1| Carbonic AnHydrase family member (cah-3) [Caenorhabditis elegans] sp|Q27504|CAH3_CAEEL Putative carbonic anhydrase 3 (Carbonate dehydratase 3) 1.0E-145
blast CAE69965 Hypothetical protein CBG16360 [Caenorhabditis briggsae] 1.0E-144
blast CAE69965 Hypothetical protein CBG16360 [Caenorhabditis briggsae] 1.0E-141
blast AAB52497 Hypothetical protein K05G3.2 [Caenorhabditis elegans] ref|NP_510675.2| K05G3.2 [Caenorhabditis elegans] 5.0E-67
blast AAA80437 Carbonic anhydrase protein 5 [Caenorhabditis elegans] ref|NP_509186.3| Carbonic AnHydrase family member (cah-5) [Caenorhabditis elegans] sp|Q10462|CAH5_CAEEL Putative carbonic anhydrase 5 precursor (Carbonate dehydratase 5) 2.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR001148 EMBL-EBI Carbonic anhydrase, eukaryotic 2.7E-55
Pfam IPR001148 EMBL-EBI Carbonic anhydrase, eukaryotic 1.6E-57
Sequence
Target Sequence 
>C. ELEGANS:189796_S_AT
gaacacccagaaatctacggcggcggccttgatcaggtctaccgcctcgtgcaataccac
ttccattggggagagaatgacaacgagggatccgagcacacgctgggtgggctccgctat
ccggccgagcttcatctcgtccaccagggagttgaagaccccggcaagctagccgttgtt
ggtgtttttcttcaacttggaaaagagggaaaggcgttgtcgaatgaggagcgtgttttg
ggaaaactttgcaatccggagacagtgactcgtgtggagaatgttaggctttccgagaag
ctgccagcaaacaagaggtcgttctggagatacgaaggatctctcaccaccccgccatgc
tcggaaatcgtcacctggaccatcttcaccgagccagtcaccgtcacccacgatcaactt
gagttgttccgtcaggtccaggacatcgagaagcgtcccatcaagaaaaactaccgtccg
actca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACACCCAGAAATCTACGGCGGCG 93 353 226 Antisense
GGCGGCCTTGATCAGGTCTACCGCC 178 541 247 Antisense
GTGCAATACCACTTCCATTGGGGAG 647 463 274 Antisense
TGACAACGAGGGATCCGAGCACACG 354 571 303 Antisense
TTCATCTCGTCCACCAGGGAGTTGA 697 679 356 Antisense
TGAAGACCCCGGCAAGCTAGCCGTT 170 471 378 Antisense
TAGCCGTTGTTGGTGTTTTTCTTCA 426 649 395 Antisense
TAGGCTTTCCGAGAAGCTGCCAGCA 204 655 510 Antisense
CGTCACCCACGATCAACTTGAGTTG 168 247 627 Antisense
TGTTCCGTCAGGTCCAGGACATCGA 625 561 650 Antisense
TCAAGAAAAACTACCGTCCGACTCA 629 629 686 Antisense
Sequence Source Affymetrix Proprietary Database