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[189789_s_at]

Description F18C12.2A /REP_DB=WormBase Gene ID /WP=CE09462 /TR=Q93529 /GB=CAA99831.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=DNA-J like domain
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 25      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 51      6grp_total > 25      4grp_total > 55      6grp_highest_sub > De      6grp_cgc > 24      6grp_high1_sub > 01_02      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 421
1 2 3 4 range
A 741.3
(3)
P 		426.8
(-1.6)
P 		447.1
(-1.3)
P 		335.2
(-3)
P 		406
B 756.4
(3)
P 		470.7
(-1.5)
P 		457.4
(-1.8)
P 		378.8
(-3)
P 		378
C 738
(3)
P 		443.3
(-3)
P 		509.4
(-1.7)
P 		443.6
(-3)
P 		295
avr 745.2
(3) 		446.9
(-2) 		471.3
(-1.6) 		385.9
(-3) 		359

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 362
1 2 3 4 5 6 range
A 597.9
(0.9)
P 		712.3
(3)
P 		392.6
(-3)
P 		572.3
(0.4)
P 		458.5
(-1.8)
P 		507.7
(-0.8)
P 		320
B 728.7
(3)
P 		611.7
(1.1)
P 		511.5
(-0.6)
P 		478.4
(-1.1)
P 		366.3
(-3)
P 		426.2
(-2)
P 		362
C 725
(3)
P 		627
(0.8)
P 		639
(1)
P 		574.7
(-0.4)
P 		462.3
(-3)
P 		529.4
(-1.5)
P 		263
avr 683.9
(3) 		650.3
(2.2) 		514.4
(-1) 		541.8
(-0.3) 		429
(-3) 		487.8
(-1.6) 		255
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 221
1 2 3 4 range
A 269.9
(3)
P 		92.6
(-1.8)
P 		116.9
(-1.2)
P 		49.1
(-3)
P 		221
B 241.2
(3)
P 		116.3
(-1.7)
P 		106
(-2.1)
P 		80.7
(-3)
P 		161
C 244.3
(3)
P 		127.4
(-1.1)
P 		177.6
(0.6)
P 		74.8
(-3)
P 		170
avr 251.8
(3) 		112.1
(-1.6) 		133.5
(-0.9) 		68.2
(-3) 		184

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 110.2
(-2.1)
P 		253.3
(3)
P 		85.5
(-3)
P 		145.2
(-0.9)
P 		138.4
(-1.1)
P 		123.6
(-1.6)
P 		168
B 253.6
(3)
P 		203.6
(0.7)
P 		124.2
(-3)
P 		126.7
(-2.9)
P 		148.8
(-1.9)
P 		158.9
(-1.4)
P 		129
C 268.7
(3)
P 		191.3
(-0)
P 		162.6
(-1.2)
P 		115.6
(-3)
P 		149.6
(-1.7)
P 		177.2
(-0.6)
P 		153
avr 210.8
(2.7) 		216.1
(3) 		124.1
(-3) 		129.2
(-2.7) 		145.6
(-1.6) 		153.2
(-1.1) 		92
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 189789_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2142
Sequence Type Exemplar sequence
Representative Public ID F18C12.2A NCBI
Target Description F18C12.2A /REP_DB=WormBase Gene ID /WP=CE09462 /TR=Q93529 /GB=CAA99831.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=DNA-J like domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059821(11), NM_001026224(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059821 NCBI Caenorhabditis elegans Receptor Mediated Endocytosis family member (rme-8) (rme-8) mRNA, complete cds. 11/11 A 
NM_001026224 NCBI Caenorhabditis elegans Receptor Mediated Endocytosis family member (rme-8) (rme-8) mRNA, complete cds. 11/11 None
SNAP00000022188 ENSEMBL cdna:SNAP chromosome:CEL140:I:8091756:8103243:1 11/11 None
GENEFINDER00000022190 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8091756:8103243:1 11/11 None
F18C12.2a ENSEMBL cdna:known chromosome:CEL140:I:8091709:8103720:1 gene:F18C12.2 11/11 A 
F18C12.2b ENSEMBL cdna:known chromosome:CEL140:I:8091756:8103243:1 gene:F18C12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8089955-8103174(+) 100.0 100.0
Public Domain and Genome References
Ensembl F18C12.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA99831 Hypothetical protein F18C12.2a [Caenorhabditis elegans] ref|NP_492222.2| Receptor Mediated Endocytosis family member (rme-8) [Caenorhabditis elegans] gb|AAK54248.1| endocytosis protein RME-8 [Caenorhabditis elegans] 0.0
blast CAA99832 Hypothetical protein F18C12.2b [Caenorhabditis elegans] ref|NP_001021395.1| Receptor Mediated Endocytosis family member (rme-8) [Caenorhabditis elegans] 0.0
blast CAE66544 Hypothetical protein CBG11852 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 6.99999986863986E-8
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.6E-14
Sequence
Target Sequence 
>C. ELEGANS:189789_S_AT
actgctgatcaattgccatctcaaggatatctaccacagttctgcactgcaatgtgtctt
caaaacacatctgcgtctcgttcagcaattctaattcttcaagaattgagcgaaaatcaa
ttctgttgtgatgctctttctcagctgccatgtattgatggaataatgaaatcaatgaag
aatcaaccaagtttgatgagagaatcggcgcacgctttaaaatgtctcatgaagagaaat
acaggagaattggctcaacaaatgctctcatgtggaatggttccatatttgttacaagtt
ttggatagttcaatgaatggagtatcaaacggagcagctgctcgtgccgagattgttgac
gcattaaaaagcgcaattttagatctgaaagttggtcagaagattgctgagattttagac
aagagtcccgtctgggcacagttta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTGCTGATCAATTGCCATCTCAAG 504 97 6136 Antisense
CTCAAGGATATCTACCACAGTTCTG 676 227 6155 Antisense
GTTCTGCACTGCAATGTGTCTTCAA 64 443 6174 Antisense
TCTTCAAAACACATCTGCGTCTCGT 143 617 6192 Antisense
GCGTCTCGTTCAGCAATTCTAATTC 173 291 6208 Antisense
CAATTCTGTTGTGATGCTCTTTCTC 151 181 6253 Antisense
CTCTTTCTCAGCTGCCATGTATTGA 215 231 6269 Antisense
ATCGGCGCACGCTTTAAAATGTCTC 561 33 6339 Antisense
GGCTCAACAAATGCTCTCATGTGGA 139 539 6387 Antisense
AGCAGCTGCTCGTGCCGAGATTGTT 624 77 6468 Antisense
AAGAGTCCCGTCTGGGCACAGTTTA 450 123 6556 Antisense
Sequence Source Affymetrix Proprietary Database