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[189788_at]

Description F46H6.2 /REP_DB=WormBase Gene ID /WP=CE25891 /TR=Q9GYP0 /GB=AAF99949.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=diaglycerol kinase
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 19      4grp_highest_8_8 > 22      4grp_high_8_8 > 14      6grp_highest_nonorm > 61      6grp_total > 04      4grp_total > 09      6grp_cgc > 02      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 418
1 2 3 4 range
A 469.9
(-3)
P 		502.5
(-2.5)
P 		515.8
(-2.3)
P 		839.7
(3)
P 		370
B 421.3
(-3)
P 		422.2
(-3)
P 		477
(-1.7)
P 		676.6
(3)
P 		255
C 521.9
(-3)
P 		547.4
(-2.2)
P 		521.8
(-3)
P 		721.7
(3)
P 		200
avr 471
(-3) 		490.7
(-2.6) 		504.9
(-2.3) 		746
(3) 		275

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 343
1 2 3 4 5 6 range
A 464.3
(0.8)
P 		525.8
(3)
P 		357.3
(-3)
P 		469.8
(1)
P 		440.2
(-0)
P 		478.9
(1.3)
P 		169
B 463.3
(0.4)
P 		482
(1)
P 		458.1
(0.2)
P 		400.6
(-1.7)
P 		540.5
(3)
P 		362.4
(-3)
P 		178
C 658.5
(3)
P 		452.2
(-0.6)
P 		419.8
(-1.2)
P 		445.9
(-0.7)
P 		435.7
(-0.9)
P 		315.2
(-3)
P 		343
avr 528.7
(3) 		486.7
(1.2) 		411.7
(-1.9) 		438.8
(-0.8) 		472.1
(0.6) 		385.5
(-3) 		143
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 171.1
(3)
P 		109.1
(-3)
P 		134.8
(-0.5)
P 		122.9
(-1.7)
P 		62
B 134.3
(1.5)
P 		104.3
(-3)
P 		110.5
(-2.1)
P 		144.2
(3)
P 		40
C 172.8
(2.1)
P 		157.4
(0.6)
P 		181.9
(3)
P 		121.7
(-3)
P 		60
avr 159.4
(3) 		123.6
(-3) 		142.4
(0.2) 		129.6
(-2) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 166
1 2 3 4 5 6 range
A 85.6
(-2.6)
P 		187
(3)
P 		77.8
(-3)
P 		119.2
(-0.7)
P 		132.8
(0)
P 		116.6
(-0.9)
P 		109
B 161.2
(-0.1)
P 		160.4
(-0.1)
P 		111.2
(-2.7)
P 		106.1
(-3)
P 		219.6
(3)
P 		135.2
(-1.5)
P 		114
C 244.1
(3)
P 		138
(-1.1)
P 		106.8
(-2.3)
P 		89.7
(-3)
P 		141
(-1)
P 		105.5
(-2.4)
P 		154
avr 163.6
(2.9) 		161.8
(2.8) 		98.6
(-3) 		105
(-2.4) 		164.5
(3) 		119.1
(-1.1) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189788_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16859
Sequence Type Exemplar sequence
Representative Public ID F46H6.2 NCBI
Target Description F46H6.2 /REP_DB=WormBase Gene ID /WP=CE25891 /TR=Q9GYP0 /GB=AAF99949.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=diaglycerol kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029510(11), NM_001029508(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029510 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-2) (dgk-2) mRNA, complete cds. 11/11 None
NM_001029508 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-2) (dgk-2) mRNA, complete cds. 11/11 None
SNAP00000026673 ENSEMBL cdna:SNAP chromosome:CEL140:X:4495198:4496747:-1 11/11 None
GENEFINDER00000026676 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4495198:4504070:-1 11/11 None
F46H6.2c ENSEMBL cdna:known chromosome:CEL140:X:4494886:4497211:-1 gene:F46H6.2 11/11 A 
F46H6.2a ENSEMBL cdna:known chromosome:CEL140:X:4495198:4504126:-1 gene:F46H6.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180720 3 2 NM_001029508 174382_s_at (11), 189788_at (11)
NM_001029510 174381_at (11), 174382_s_at (11), 189788_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4495136-4504071(-) 99.93 99.93
Public Domain and Genome References
Ensembl F46H6.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAP40526 Diacylglycerol kinase protein 2, isoform a [Caenorhabditis elegans] ref|NP_001024679.1| DiacylGlycerol Kinase family member (dgk-2) [Caenorhabditis elegans] 0.0
blast AAP40528 Diacylglycerol kinase protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024681.1| DiacylGlycerol Kinase family member (dgk-2) [Caenorhabditis elegans] 0.0
blast CAE68602 Hypothetical protein CBG14478 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.3E-34
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 4.0E-38
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.3E-34
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 4.9E-5
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 1.4E-6
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 4.0E-38
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 1.7E-31
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 4.0E-38
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024679.1 1 9-26
F46H6.2a 1 9-26
GENEFINDER00000026676 1 9-26
Sequence
Target Sequence 
>C. ELEGANS:189788_AT
acaaactacgtgtcggttggtgtcgatgcttgtgtcacgttaggaatgcaaaacacaaga
gaatcaattcccagagcaatgagtagcaggctactcaacaaattcctcttcttcacattt
ggaaccaaggatgtttttgagagagtttgcaagggtctgaacgagagaatcgacctatat
ttagacgacgtccacgtcaaccttcccgatatcgaaggtctcatctttctcaacattcca
tattggggcgctggagtcaagccatgggccacgtacaatgatagtcatcgacaagaatgc
gacgatgagatgattgaagtgtttgcggtgacatcatcgtttcatattgctcaaatgcag
atcggactggcgagcccgttgtgcattgggcaggccaagcatgcgaagct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAAACTACGTGTCGGTTGGTGTCG 226 109 841 Antisense
GGTGTCGATGCTTGTGTCACGTTAG 92 497 859 Antisense
GGCTACTCAACAAATTCCTCTTCTT 68 539 929 Antisense
TTAGACGACGTCCACGTCAACCTTC 421 545 1021 Antisense
TATCGAAGGTCTCATCTTTCTCAAC 125 659 1050 Antisense
TGGAGTCAAGCCATGGGCCACGTAC 132 549 1092 Antisense
GAAGTGTTTGCGGTGACATCATCGT 628 335 1156 Antisense
GACATCATCGTTTCATATTGCTCAA 214 371 1170 Antisense
AATGCAGATCGGACTGGCGAGCCCG 522 161 1194 Antisense
GCGAGCCCGTTGTGCATTGGGCAGG 481 287 1210 Antisense
TTGGGCAGGCCAAGCATGCGAAGCT 516 703 1226 Antisense
Sequence Source Affymetrix Proprietary Database