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[189777_at] actin-like protein

Description C49H3.8 /REP_DB=WormBase Gene ID /WP=CE04234 /TR=Q9GYR2 /GB=AAF99891.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=actin-like protein
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 84      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 82      6grp_total > 74      4grp_total > 62      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 163
1 2 3 4 range
A 309.1
(3)
P 		271.5
(1)
P 		262.9
(0.6)
P 		193.5
(-3)
P 		116
B 334.2
(3)
P 		257.9
(-2.2)
P 		309.5
(1.3)
P 		246.5
(-3)
P 		88
C 332.8
(1.7)
P 		273.6
(-1.6)
P 		356.8
(3)
P 		247.2
(-3)
P 		110
avr 325.4
(3) 		267.7
(-0.6) 		309.7
(2) 		229.1
(-3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 251
1 2 3 4 5 6 range
A 198.2
(-3)
P 		337.7
(1.5)
P 		385.5
(3)
P 		351.8
(1.9)
P 		341.1
(1.6)
P 		327.5
(1.1)
P 		187
B 303.8
(-3)
P 		312.8
(-2.5)
P 		343.2
(-0.9)
P 		367.5
(0.3)
P 		410.2
(2.6)
P 		418.3
(3)
P 		115
C 381.1
(0.7)
P 		270
(-3)
P 		377.9
(0.6)
P 		413
(1.8)
P 		388
(1)
P 		449.2
(3)
P 		179
avr 294.4
(-3) 		306.8
(-2.3) 		368.9
(1.3) 		377.4
(1.8) 		379.8
(1.9) 		398.3
(3) 		104
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 112.6
(3)
P 		58.9
(-0.8)
P 		68.7
(-0.1)
P 		28.3
(-3)
P 		84
B 106.6
(3)
P 		63.7
(-1.8)
P 		71.7
(-0.9)
P 		52.5
(-3)
P 		54
C 110.2
(2)
P 		78.6
(-0.3)
P 		124.3
(3)
P 		41.7
(-3)
P 		83
avr 109.8
(3) 		67.1
(-0.7) 		88.2
(1.1) 		40.8
(-3) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 36.5
(-3)
P 		120.1
(3)
P 		83.9
(0.4)
P 		89.2
(0.8)
P 		102.9
(1.8)
P 		79.7
(0.1)
P 		84
B 105.7
(-1.4)
P 		104.1
(-1.5)
P 		83.3
(-3)
P 		97.3
(-2)
P 		166.7
(3)
P 		156
(2.2)
P 		83
C 141.3
(2.2)
P 		82.4
(-3)
P 		96.2
(-1.8)
P 		83.1
(-2.9)
P 		125.5
(0.8)
P 		150.4
(3)
P 		68
avr 94.5
(-2.1) 		102.2
(-1) 		87.8
(-3) 		89.9
(-2.7) 		131.7
(3) 		128.7
(2.6) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189777_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8960
Sequence Type Exemplar sequence
Representative Public ID C49H3.8 NCBI
Target Description C49H3.8 /REP_DB=WormBase Gene ID /WP=CE04234 /TR=Q9GYR2 /GB=AAF99891.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=actin-like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068913(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068913 NCBI Caenorhabditis elegans C49H3.8 (C49H3.8) mRNA, complete cds. 11/11 None
SNAP00000028664 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7900264:7902643:-1 11/11 None
C49H3.8 ENSEMBL cdna:known chromosome:CEL140:IV:7900264:7901974:-1 gene:C49H3.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000028675 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7900269-7901980(-) 100.0 100.0
Public Domain and Genome References
Gene Title actin-like protein
Ensembl C49H3.8
Entrez Gene 183628 Entrez gene
SwissProt Q9GYR2 EMBL-EBI
WormBase CE04234 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
15629 actin cytoskeleton inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5200 structural constituent of cytoskeleton inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF99891 Hypothetical protein C49H3.8 [Caenorhabditis elegans] ref|NP_501314.1| C49H3.8 [Caenorhabditis elegans] 1.0E-173
blast CAE70904 Hypothetical protein CBG17701 [Caenorhabditis briggsae] 1.0E-140
blast CAE70904 Hypothetical protein CBG17701 [Caenorhabditis briggsae] 1.0E-139
blast EAA00312 ENSANGP00000016785 [Anopheles gambiae str. PEST] ref|XP_320451.2| ENSANGP00000016785 [Anopheles gambiae str. PEST] 9.0E-35
blast AAH45412 Actin-related protein 10 homolog [Danio rerio] ref|NP_956464.1| actin-related protein 10 homolog [Danio rerio] 3.0E-21
Protein Domains
Database ID Description E-Value
Pfam IPR004000 EMBL-EBI Actin/actin-like 5.6E-7
Pfam IPR004000 EMBL-EBI Actin/actin-like 5.6E-7
Sequence
Target Sequence 
>C. ELEGANS:189777_AT
acgcttagtttatcactcatttgtctggataaggaacgggccgaaaattggacaaaatgg
gaagcgtcagaagaaggagaaaaaccggatattgagacactttgcaaagaaagactattt
ccgaataatggaaaaatatttgtagttcctccagttgtattcgaaacaagtactgaaatc
ttcttcaatgagaatctcaatcctacatcattcgatctttcccttccgaaacttcttcac
aagctggtatcaaaatgcccgattgatctccggaaaaagctattcccaaatatactttta
actggtggagtctctacaattcctggtctcatgaaacgtttggaacaagaaattcaagtt
atcgacgagaaaaacaattccaaaattgccgtaattgtaaaattctatcaattttcggaa
atcaaaaacacgccacttttcgtttcctggctcggtgcaagtctattgggatcacttcga
gaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGCTTAGTTTATCACTCATTTGTC 543 91 358 Antisense
AAACCGGATATTGAGACACTTTGCA 181 125 439 Antisense
TTGTAGTTCCTCCAGTTGTATTCGA 505 705 497 Antisense
AATCCTACATCATTCGATCTTTCCC 586 167 556 Antisense
TTCCGAAACTTCTTCACAAGCTGGT 682 697 581 Antisense
CAAAATGCCCGATTGATCTCCGGAA 536 189 608 Antisense
TGGAGTCTCTACAATTCCTGGTCTC 634 549 663 Antisense
ATTCCTGGTCTCATGAAACGTTTGG 609 7 676 Antisense
TTCGGAAATCAAAAACACGCCACTT 409 695 771 Antisense
CTGGCTCGGTGCAAGTCTATTGGGA 252 241 804 Antisense
AGTCTATTGGGATCACTTCGAGAGA 685 61 817 Antisense
Sequence Source Affymetrix Proprietary Database