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[189763_at] RAS-related protein

Description C25D7.7 /REP_DB=WormBase Gene ID /WP=CE08393 /TR=O17599 /GB=CAB02777.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 89      4grp_highest_8_8 > 07      4grp_high_8_8 > 20      6grp_highest_nonorm > 91      6grp_total > 99      4grp_total > 19      6grp_highest_sub > Cu      6grp_cgc > 79      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 244.8
(-2.7)
P 		326.1
(3)
P 		240
(-3)
P 		318
(2.4)
P 		86
B 250.1
(-3)
P 		274.1
(-1.9)
P 		273.2
(-1.9)
P 		379.3
(3)
P 		129
C 323.5
(0.9)
P 		318.9
(0.7)
P 		241.5
(-3)
P 		367.6
(3)
P 		126
avr 272.8
(-1.8) 		306.4
(0.2) 		251.6
(-3) 		355
(3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 174.4
(-1.6)
P 		247.5
(2)
P 		197.1
(-0.5)
P 		254.8
(2.4)
P 		146.6
(-3)
P 		266.9
(3)
P 		120
B 165.5
(-3)
P 		175.6
(-2.6)
P 		176.6
(-2.6)
P 		166.7
(-3)
P 		315.8
(3)
P 		226
(-0.6)
P 		150
C 313.5
(3)
P 		198
(-2.1)
P 		180.5
(-2.8)
P 		176.6
(-3)
P 		294.7
(2.2)
P 		240.4
(-0.2)
P 		137
avr 217.8
(-0.1) 		207
(-1) 		184.7
(-3) 		199.4
(-1.7) 		252.4
(3) 		244.4
(2.3) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 89.1
(3)
P 		70.8
(0.4)
P 		62.7
(-0.7)
P 		46.6
(-3)
P 		43
B 79.7
(2.6)
P 		67.7
(-1.5)
P 		63.3
(-3)
P 		80.8
(3)
P 		18
C 107.1
(3)
P 		91.7
(1)
P 		84.2
(-0)
P 		62
(-3)
P 		45
avr 92
(3) 		76.7
(-0.2) 		70.1
(-1.5) 		63.1
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 96
1 2 3 4 5 6 range
A 32.1
(-3)
P 		88
(3)
P 		42.9
(-1.8)
P 		64.6
(0.5)
P 		44.2
(-1.7)
P 		65
(0.5)
P 		56
B 57.6
(-2)
P 		58.5
(-1.9)
P 		42.9
(-3)
P 		44.2
(-2.9)
P 		128.3
(3)
P 		84.3
(-0.1)
P 		85
C 116.2
(3)
P 		60.4
(-1.1)
P 		45.9
(-2.2)
P 		35.5
(-3)
P 		95.4
(1.5)
P 		80.5
(0.3)
P 		81
avr 68.6
(0.3) 		69
(0.3) 		43.9
(-3) 		48.1
(-2.4) 		89.3
(3) 		76.6
(1.3) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189763_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14187
Sequence Type Exemplar sequence
Representative Public ID C25D7.7 NCBI
Target Description C25D7.7 /REP_DB=WormBase Gene ID /WP=CE08393 /TR=O17599 /GB=CAB02777.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074306(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074306 NCBI Caenorhabditis elegans RAP homolog (vertebrate Rap GTPase family) family member (rap-2) (rap-2) mRNA, complete cds. 10/11 None
SNAP00000019712 ENSEMBL cdna:SNAP chromosome:CEL140:V:15064484:15066658:-1 10/11 None
GENEFINDER00000019727 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15065150:15066658:-1 10/11 None
C25D7.7 ENSEMBL cdna:known chromosome:CEL140:V:15064484:15066658:-1 gene:C25D7.7 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15064483-15066658(-) 100.0 100.0
Public Domain and Genome References
Gene Title RAS-related protein
Gene Symbol rap-2
Ensembl C25D7.7
Entrez Gene 180011 Entrez gene
SwissProt O17599 EMBL-EBI
WormBase CE35831 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGAAFFX.10815.1.S1_AT similar to rap2A-like protein gga
HG-U133_PLUS_2:214488_AT RAP2B, member of RAS oncogene family hs
HG-U133A_2:214488_AT RAP2B, member of RAS oncogene family hs
HG-U133A:214488_AT RAP2B, member of RAS oncogene family hs
HG-U133_PLUS_2:238622_AT RAP2B, member of RAS oncogene family hs
HG-U133B:238622_AT RAP2B, member of RAS oncogene family hs
HG-U95AV2:31696_AT RAP2B, member of RAS oncogene family hs
HU35KSUBA:R39394_AT RAP2B, member of RAS oncogene family hs
HU35KSUBA:AA490572_AT RAP2B, member of RAS oncogene family hs
HU35KSUBD:RC_AA598432_AT RAP2B, member of RAS oncogene family hs
HU35KSUBD:RC_AA446133_AT RAP2B, member of RAS oncogene family hs
U133_X3P:HS.98643.0.A1_3P_AT RAP2B, member of RAS oncogene family hs
U133_X3P:HS.239527.0.S1_3P_AT RAP2B, member of RAS oncogene family hs
U133_X3P:HS.155218.3.A1_3P_AT RAP2B, member of RAS oncogene family hs
HG-U133A_2:213923_AT RAP2B, member of RAS oncogene family hs
HG-FOCUS:213923_AT RAP2B, member of RAS oncogene family hs
HG-U133_PLUS_2:213923_AT RAP2B, member of RAS oncogene family hs
HG-U133A:213923_AT RAP2B, member of RAS oncogene family hs
HG-U133A_2:214487_S_AT RAP2B, member of RAS oncogene family hs
HG-FOCUS:214487_S_AT RAP2B, member of RAS oncogene family hs
HG-U133_PLUS_2:214487_S_AT RAP2B, member of RAS oncogene family hs
HG-U133A:214487_S_AT RAP2B, member of RAS oncogene family hs
HG-U95B:43765_AT RAP2B, member of RAS oncogene family hs
HG-U95B:43766_G_AT RAP2B, member of RAS oncogene family hs
HG-U95B:56039_AT RAP2B, member of RAS oncogene family hs
HG-U133_PLUS_2:227897_AT RAP2B, member of RAS oncogene family hs
HG-U133B:227897_AT RAP2B, member of RAS oncogene family hs
HU35KSUBA:H72545_AT RAP2B, member of RAS oncogene family hs
U133_X3P:HS.155218.2.S1_3P_AT RAP2B, member of RAS oncogene family hs
MG-U74AV2:99874_AT RAP2B, member of RAS oncogene family mm
MU11KSUBA:AA408555_AT RAP2B, member of RAS oncogene family mm
MU11KSUBB:MSA.5927.0_S_AT RAP2B, member of RAS oncogene family mm
MG-U74AV2:104020_AT RAP2B, member of RAS oncogene family mm
MG-U74BV2:164066_AT RAP2B, member of RAS oncogene family mm
MOE430A:1439548_AT RAP2B, member of RAS oncogene family mm
MOUSE430A_2:1439548_AT RAP2B, member of RAS oncogene family mm
MOUSE430_2:1439548_AT RAP2B, member of RAS oncogene family mm
MOE430A:1417915_AT RAP2B, member of RAS oncogene family mm
MOUSE430A_2:1417915_AT RAP2B, member of RAS oncogene family mm
MOUSE430_2:1417915_AT RAP2B, member of RAS oncogene family mm
MOE430A:1417914_AT RAP2B, member of RAS oncogene family mm
MOUSE430A_2:1417914_AT RAP2B, member of RAS oncogene family mm
MOUSE430_2:1417914_AT RAP2B, member of RAS oncogene family mm
MOE430A:1448885_AT RAP2B, member of RAS oncogene family mm
MOUSE430A_2:1448885_AT RAP2B, member of RAS oncogene family mm
MOUSE430_2:1448885_AT RAP2B, member of RAS oncogene family mm
MG-U74CV2:140455_AT RAP2B, member of RAS oncogene family (Rap2b), mRNA mm
MU19KSUBC:TC35848_AT RAP2B, member of RAS oncogene family (Rap2b), mRNA mm
RAE230A:1369614_AT RAP2B, member of RAS oncogene family rn
RAT230_2:1369614_AT RAP2B, member of RAS oncogene family rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02777 Hypothetical protein C25D7.7 [Caenorhabditis elegans] ref|NP_506707.2| RAP homolog (vertebrate Rap GTPase family) family member (rap-2) [Caenorhabditis elegans] 2.0E-90
blast CAE66326 Hypothetical protein CBG11577 [Caenorhabditis briggsae] 8.0E-89
blast CAB02777 Hypothetical protein C25D7.7 [Caenorhabditis elegans] ref|NP_506707.2| RAP homolog (vertebrate Rap GTPase family) family member (rap-2) [Caenorhabditis elegans] 7.0E-62
blast CAE66326 Hypothetical protein CBG11577 [Caenorhabditis briggsae] 7.0E-62
blast CAA37178 unnamed protein product [Homo sapiens] 1.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.2E-90
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 3.6E-72
Sequence
Target Sequence 
>C. ELEGANS:189763_AT
ggtcgttctgggtagtggaggtgtcggaaaatcggcgttgacggtgcaatttgtcagtag
cacattcatcgagaagtatgatccgacgattgaggacttttatcgcaaggaaattgaagt
ggacggtcaaccatcagtactggaaattctggacactgcaggaacggaacaattttcttc
gatgcgggatctttatatcaaaaatggacagggcttcgttgttgtctactcgatcaccag
tcaacagacgtttcatgatattcgaaatatgaaagagcaaattgtcagggttaagggatc
cgaaaatgtgccgattttgttagttggaaacaaatgcgatctttcacatcagagacaagt
acgatcagaggaaggattggcgttggaaaattcaaaacacaaagtttttaacattttatc
aaattttgtttgggaaaaaatcattttctggaaaaatcgattttttttcaaaatagaaac
cgaaagttggtcatgtccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCGTTCTGGGTAGTGGAGGTGTC 642 499 30 Antisense
AAATCGGCGTTGACGGTGCAATTTG 562 117 58 Antisense
GCAATTTGTCAGTAGCACATTCATC 309 323 75 Antisense
AAGTGGACGGTCAACCATCAGTACT 232 159 146 Antisense
GAAATTCTGGACACTGCAGGAACGG 38 365 172 Antisense
GAACAATTTTCTTCGATGCGGGATC 490 353 196 Antisense
TGGACAGGGCTTCGTTGTTGTCTAC 56 549 234 Antisense
TTGTCTACTCGATCACCAGTCAACA 24 709 251 Antisense
CACCAGTCAACAGACGTTTCATGAT 134 203 264 Antisense
AATGCGATCTTTCACATCAGAGACA 358 167 362 Antisense
GAAACCGAAAGTTGGTCATGTCCAT 166 357 505 Antisense
Sequence Source Affymetrix Proprietary Database