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[189759_at] phosphate permease

Description B0222.2 /REP_DB=WormBase Gene ID /WP=CE06693 /TR=Q17454 /GB=AAA92319.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=phosphate permease
Cluster 6grp_total > 46      4grp_total > 03      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 11.8
(-2.2)
A 		6.6
(-3)
A 		10.4
(-2.4)
A 		47.8
(3)
A 		41
B 12.7
(-2.1)
A 		11.9
(-2.3)
A 		9.4
(-3)
A 		32
(3)
A 		23
C 9
(-2.3)
A 		5.8
(-2.7)
A 		3.3
(-3)
A 		51.1
(3)
A 		48
avr 11.2
(-2.4) 		8.1
(-2.9) 		7.7
(-3) 		43.6
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 19.9
(0.5)
A 		3.9
(-3)
A 		9.1
(-1.9)
A 		31.3
(3)
A 		12.5
(-1.1)
A 		5.2
(-2.7)
A 		27
B 2.1
(-3)
A 		39.3
(1.6)
A 		12.3
(-1.7)
A 		10.8
(-1.9)
A 		10.5
(-2)
A 		50.9
(3)
A 		49
C 18.7
(3)
A 		12.5
(0)
A 		7
(-2.6)
A 		17.7
(2.5)
A 		8.1
(-2)
A 		6.1
(-3)
A 		13
avr 13.6
(-0.8) 		18.6
(1.9) 		9.5
(-3) 		19.9
(2.6) 		10.4
(-2.5) 		20.7
(3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 4.3
(0.1)
A 		1.4
(-3)
A 		2.7
(-1.6)
A 		7
(3)
A 		6
B 4.1
(-0.5)
A 		2.9
(-2.1)
A 		2.2
(-3)
A 		6.8
(3)
A 		5
C 3
(-1.5)
A 		1.7
(-2.5)
A 		1.1
(-3)
A 		8.6
(3)
A 		8
avr 3.8
(-1) 		2
(-3) 		2
(-3) 		7.5
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 3.7
(-0.8)
A 		1.4
(-2.9)
A 		2
(-2.4)
A 		7.9
(3)
A 		3.8
(-0.7)
A 		1.3
(-3)
A 		7
B 0.7
(-3)
A 		13.1
(1.1)
A 		3
(-2.2)
A 		2.9
(-2.3)
A 		4.3
(-1.8)
A 		19
(3)
A 		18
C 6.9
(3)
A 		3.8
(-0.6)
A 		1.8
(-3)
A 		3.6
(-0.9)
A 		2.6
(-2.1)
A 		2
(-2.8)
A 		5
avr 3.8
(-1.2) 		6.1
(1.5) 		2.3
(-3) 		4.8
(-0.1) 		3.6
(-1.5) 		7.4
(3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189759_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11876
Sequence Type Exemplar sequence
Representative Public ID B0222.2 NCBI
Target Description B0222.2 /REP_DB=WormBase Gene ID /WP=CE06693 /TR=Q17454 /GB=AAA92319.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=phosphate permease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072969(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072969 NCBI Caenorhabditis elegans B0222.2 (B0222.2) mRNA, complete cds. 11/11 None
SNAP00000023003 ENSEMBL cdna:SNAP chromosome:CEL140:V:9136839:9140304:-1 9/11 None
GENEFINDER00000023013 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9138242:9140304:-1 11/11 None
B0222.2 ENSEMBL cdna:known chromosome:CEL140:V:9138242:9140304:-1 gene:B0222.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9138242-9140305(-) 100.0 100.0
Public Domain and Genome References
Gene Title phosphate permease
Ensembl B0222.2
Entrez Gene 181857 Entrez gene
SwissProt Q17454 EMBL-EBI
WormBase CE35207 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6817 phosphate transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5315 inorganic phosphate transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA92319 Hypothetical protein B0222.2 [Caenorhabditis elegans] ref|NP_505370.2| B0222.2 [Caenorhabditis elegans] 0.0
blast AAA92320 Hypothetical protein B0222.3 [Caenorhabditis elegans] ref|NP_505371.2| B0222.3 [Caenorhabditis elegans] 0.0
blast CAE72112 Hypothetical protein CBG19205 [Caenorhabditis briggsae] 0.0
blast AAA92319 Hypothetical protein B0222.2 [Caenorhabditis elegans] ref|NP_505370.2| B0222.2 [Caenorhabditis elegans] 2.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR001204 EMBL-EBI Phosphate transporter 1.0E-126
Pfam IPR001204 EMBL-EBI Phosphate transporter 1.9E-6
Pfam IPR001204 EMBL-EBI Phosphate transporter 8.8E-11
Pfam IPR001204 EMBL-EBI Phosphate transporter 2.8E-104
Pfam IPR001204 EMBL-EBI Phosphate transporter 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505370.2 9 27-46,66-88,108-130,135-152,164-186,201-223,362-384,416-438,450-472
B0222.2 9 27-46,66-88,108-130,135-152,164-186,201-223,362-384,416-438,450-472
SNAP00000023003 3 25-47,101-123,246-268
GENEFINDER00000023013 9 27-46,66-88,108-130,164-186,199-221,234-256,379-401,433-455,467-489
Sequence
Target Sequence 
>C. ELEGANS:189759_AT
gtacaacaacgaaagactccatctggaaaaattcgaaaattctatgtttggcttctccca
gaccgtactcgtgttgatggtcgtaacatcacaaagttattttccacaattcaagtgttc
acagcgtgttttgctggatttgctcatggagccaatgatgtcagtaacgctattgctccg
cttgcagcaattatttcaatctatcgaacaaagtctgtcgagcaaaccgaatcagttcca
atttatgtcctcttatatggagtatttgctatttgtgtaggattatggttattcggtcat
tatgttataaaaaccgtcggtacaaatatgtcggaaatcaatccagcaagtggattcaca
attgaatttggagcagcgatgacatcacttcttgccagtaaagtgggaagtgtggtggca
gttggaatgatacgttctgaaaatggtgttaaatggtcaatatttcgagatattgccata
tcgtgggttgtcactctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTACAACAACGAAAGACTCCATCTG 164 461 937 Antisense
AAATTCTATGTTTGGCTTCTCCCAG 602 115 973 Antisense
CTCCCAGACCGTACTCGTGTTGATG 695 233 991 Antisense
TCAAGTGTTCACAGCGTGTTTTGCT 193 633 1047 Antisense
ATGTCAGTAACGCTATTGCTCCGCT 325 47 1094 Antisense
TGCTCCGCTTGCAGCAATTATTTCA 691 581 1110 Antisense
GTCGAGCAAACCGAATCAGTTCCAA 214 473 1153 Antisense
TCAGTTCCAATTTATGTCCTCTTAT 448 623 1168 Antisense
GCAGCGATGACATCACTTCTTGCCA 269 315 1309 Antisense
GTGGTGGCAGTTGGAATGATACGTT 239 489 1348 Antisense
TGCCATATCGTGGGTTGTCACTCTT 414 585 1410 Antisense
Sequence Source Affymetrix Proprietary Database