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[189746_at] glycosyl hydrolase

Description E02H9.5 /REP_DB=WormBase Gene ID /WP=CE09122 /TR=Q9TZA0 /GB=AAC68766.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=glycosyl hydrolase
Cluster 6grp_total > 43      4grp_total > 53      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 77.2
(0.8)
A 		101.3
(3)
M 		41.9
(-2.4)
A 		35.3
(-3)
A 		66
B 56.6
(1.6)
A 		39.3
(-1.5)
A 		64.3
(3)
P 		30.8
(-3)
A 		34
C 83.2
(3)
P 		49.4
(-3)
A 		76.4
(1.8)
P 		50
(-2.9)
A 		34
avr 72.3
(3) 		63.3
(1.4) 		60.9
(1) 		38.7
(-3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 63.7
(-2.2)
P 		90.4
(3)
P 		89.9
(2.9)
P 		72.1
(-0.6)
A 		76.1
(0.2)
A 		59.6
(-3)
A 		31
B 59.5
(-3)
P 		101.2
(3)
P 		84.6
(0.6)
P 		68.4
(-1.7)
A 		64
(-2.4)
P 		70.9
(-1.4)
P 		42
C 90.4
(0.4)
P 		77.8
(-0.8)
P 		118.1
(3)
P 		55.2
(-3)
M 		91
(0.4)
M 		64.6
(-2.1)
P 		63
avr 71.2
(-1.9) 		89.8
(1.6) 		97.5
(3) 		65.2
(-3) 		77
(-0.8) 		65
(-3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 28.1
(3)
A 		22
(1.4)
M 		11
(-1.5)
A 		5.2
(-3)
A 		23
B 18
(3)
A 		9.7
(-1.4)
A 		14.9
(1.4)
P 		6.6
(-3)
A 		11
C 27.5
(3)
P 		14.2
(-1.2)
A 		26.6
(2.7)
P 		8.4
(-3)
A 		19
avr 24.5
(3) 		15.3
(-0.1) 		17.5
(0.6) 		6.7
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 11.8
(-3)
P 		32.2
(3)
P 		19.6
(-0.7)
P 		18.3
(-1.1)
A 		23
(0.3)
A 		14.5
(-2.2)
A 		20
B 20.7
(-2)
P 		33.7
(3)
P 		20.5
(-2.1)
P 		18.1
(-3)
A 		26
(0)
P 		26.4
(0.2)
P 		16
C 33.5
(3)
P 		23.7
(0.4)
P 		30.1
(2.1)
P 		11.1
(-3)
M 		29.4
(1.9)
M 		21.6
(-0.2)
P 		22
avr 22
(-0.4) 		29.9
(3) 		23.4
(0.2) 		15.8
(-3) 		26.1
(1.4) 		20.8
(-0.9) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189746_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7319
Sequence Type Exemplar sequence
Representative Public ID E02H9.5 NCBI
Target Description E02H9.5 /REP_DB=WormBase Gene ID /WP=CE09122 /TR=Q9TZA0 /GB=AAC68766.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=glycosyl hydrolase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065157(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065157 NCBI Caenorhabditis elegans E02H9.5 (E02H9.5) mRNA, complete cds. 11/11 None
SNAP00000038954 ENSEMBL cdna:SNAP chromosome:CEL140:III:2446211:2448523:1 11/11 None
GENEFINDER00000038963 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2446211:2448523:1 11/11 None
E02H9.5 ENSEMBL cdna:known chromosome:CEL140:III:2446211:2448523:1 gene:E02H9.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2446210-2448523(+) 100.0 100.0
Public Domain and Genome References
Gene Title glycosyl hydrolase
Ensembl E02H9.5
Entrez Gene 184000 Entrez gene
SwissProt Q9TZA0 EMBL-EBI
WormBase CE09122 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:249243_S_AT glycosyl hydrolase family 1 protein at
CANINE_2:CFAAFFX.8606.1.S1_AT similar to lactase-phlorizin hydrolase preproprotein cfa
CANINE_2:CFAAFFX.8618.1.S1_S_AT similar to lactase-phlorizin hydrolase preproprotein cfa
CANINE_2:CFAAFFX.8622.1.S1_S_AT similar to lactase-phlorizin hydrolase preproprotein cfa
CANINE_2:CFAAFFX.8630.1.S1_S_AT similar to lactase-phlorizin hydrolase preproprotein cfa
DROSOPHILA_2:1633834_AT dm
DROSGENOME1:141360_AT dm
CHICKEN:GGA.18117.1.S1_AT lactase gga
CHICKEN:GGA.613.1.S1_AT lactase gga
CHICKEN:GGAAFFX.23848.1.S1_AT lactase gga
CHICKEN:GGAAFFX.7809.1.S1_AT lactase gga
HG-U133_PLUS_2:206945_AT lactase hs
HG-U133A:206945_AT lactase hs
HG-FOCUS:206945_AT lactase hs
HG-U133A_2:206945_AT lactase hs
HUGENEFL:X07994_AT lactase hs
U133_X3P:G4504966_3P_AT lactase hs
HG-U95AV2:32917_AT lactase hs
U133_X3P:HS2.234571.1.S1_3P_AT Lactase hs
HG-U133_PLUS_2:1557386_AT Lactase hs
MU11KSUBA:AA521747_S_AT lactase mm
MG-U74AV2:104018_AT lactase mm
MOE430A:1439479_AT lactase mm
MOUSE430A_2:1439479_AT lactase mm
MOUSE430_2:1439479_AT lactase mm
RG-U34A:L04635EXON_S_AT lactase rn
RG-U34A:X56747CDS_AT lactase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4553 hydrolase activity, hydrolyzing O-glycosyl compounds inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC68766 Hypothetical protein E02H9.5 [Caenorhabditis elegans] ref|NP_497558.1| E02H9.5 [Caenorhabditis elegans] 0.0
blast AAA83309 Hypothetical protein C50F7.10 [Caenorhabditis elegans] ref|NP_501271.1| C50F7.10 [Caenorhabditis elegans] 0.0
blast CAE70870 Hypothetical protein CBG17658 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001360 EMBL-EBI Glycoside hydrolase, family 1 1.2E-66
Pfam IPR001360 EMBL-EBI Glycoside hydrolase, family 1 7.2E-65
Sequence
Target Sequence 
>C. ELEGANS:189746_AT
ggttcgggagattttccagtgctgatgagggagagaatgccgtttataccgaagtttagt
gaggaggagaaggagattattaaaggaagcactgacttcatcggaatcaactactacctc
agcttcctggttcgcgcgccgaaagatggtgagaaagcttcttcccagtctcagcatgac
ggtggttatgtgtttgtggagggaaaatgggataaaatatgcggcgaaacctggattcgt
tacgcccctgatgggctcctaaaaattttgagatatgtcaaggaaaagtatgcaaatact
ccggtgtttataaccgaaaatggatgcatggatatagtcgggcaggaccaagaagatgct
tttcacgatcaacatcgaattgattacatctctgggcatttggaagcggtggcgaaagct
ctcgatgaaggctgcaacgtgatcggttacactgtttggacgctaatggacaacttcgag
tgggatgacggttttgagctcaagttcgggctctgtgaagtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTCGGGAGATTTTCCAGTGCTGA 494 507 823 Antisense
AGCACTGACTTCATCGGAATCAACT 117 77 910 Antisense
GGAATCAACTACTACCTCAGCTTCC 599 533 925 Antisense
TGGTGAGAAAGCTTCTTCCCAGTCT 342 553 969 Antisense
CTTCCCAGTCTCAGCATGACGGTGG 101 215 983 Antisense
GCGGCGAAACCTGGATTCGTTACGC 454 291 1043 Antisense
TTACGCCCCTGATGGGCTCCTAAAA 108 657 1062 Antisense
GAAGCGGTGGCGAAAGCTCTCGATG 98 343 1225 Antisense
GAAGGCTGCAACGTGATCGGTTACA 218 333 1249 Antisense
GATCGGTTACACTGTTTGGACGCTA 167 419 1263 Antisense
GCTCAAGTTCGGGCTCTGTGAAGTC 177 301 1320 Antisense
Sequence Source Affymetrix Proprietary Database