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[189740_at] Alpha-mannosidase

Description F55D10.1 /REP_DB=WormBase Gene ID /WP=CE28456 /TR=Q20829 /GB=AAA81731.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Alpha-mannosidase
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 62      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 71      6grp_total > 62      4grp_total > 42      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 268
1 2 3 4 range
A 141.4
(-3)
P 		398.5
(3)
P 		227
(-1)
P 		200
(-1.6)
P 		257
B 131.4
(-3)
P 		362.6
(3)
P 		260.9
(0.4)
P 		136.8
(-2.9)
P 		231
C 130.2
(-3)
P 		301.2
(3)
P 		294.7
(2.8)
P 		140.8
(-2.6)
P 		171
avr 134.3
(-3) 		354.1
(3) 		260.9
(0.5) 		159.2
(-2.3) 		220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 363
1 2 3 4 5 6 range
A 238.6
(-0.6)
P 		255.6
(0)
P 		343.7
(3)
P 		303.7
(1.6)
P 		245.9
(-0.3)
P 		166.8
(-3)
P 		177
B 173.2
(-3)
P 		271
(-1.3)
P 		519.8
(3)
P 		350.3
(0.1)
P 		239.1
(-1.9)
P 		248.3
(-1.7)
P 		347
C 156.9
(-3)
P 		278.3
(-0.4)
P 		397.9
(2.2)
P 		434.2
(3)
P 		229.8
(-1.4)
P 		180.8
(-2.5)
P 		277
avr 189.6
(-3) 		268.3
(-1) 		420.5
(3) 		362.7
(1.5) 		238.3
(-1.7) 		198.6
(-2.8) 		231
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 51.5
(-0.7)
P 		86.5
(3)
P 		59.3
(0.1)
P 		29.3
(-3)
P 		57
B 41.9
(-1.7)
P 		89.6
(3)
P 		60.5
(0.1)
P 		29.2
(-3)
P 		60
C 43.1
(-1.5)
P 		86.6
(1.8)
P 		102.7
(3)
P 		23.7
(-3)
P 		79
avr 45.5
(-1.2) 		87.6
(3) 		74.2
(1.7) 		27.4
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 44
(-2.6)
P 		90.9
(3)
P 		74.8
(1.1)
P 		77
(1.3)
P 		74.2
(1)
P 		40.6
(-3)
P 		50
B 60.3
(-3)
P 		90.2
(-0.3)
P 		126.2
(3)
P 		92.8
(-0)
P 		97.1
(0.4)
P 		92.6
(-0.1)
P 		66
C 58.1
(-3)
P 		84.9
(0.7)
P 		101.3
(3)
P 		87.3
(1.1)
P 		74.4
(-0.7)
P 		60.5
(-2.7)
P 		43
avr 54.1
(-3) 		88.7
(1.4) 		100.8
(3) 		85.7
(1.1) 		81.9
(0.6) 		64.6
(-1.7) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 189740_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16109
Sequence Type Exemplar sequence
Representative Public ID F55D10.1 NCBI
Target Description F55D10.1 /REP_DB=WormBase Gene ID /WP=CE28456 /TR=Q20829 /GB=AAA81731.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Alpha-mannosidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076410(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076410 NCBI Caenorhabditis elegans F55D10.1 (Alpha-mannosidase) mRNA, complete cds. 11/11 None
SNAP00000004381 ENSEMBL cdna:SNAP chromosome:CEL140:X:4733542:4738461:1 10/11 None
GENEFINDER00000004387 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4733542:4738461:1 9/11 None
F55D10.1 ENSEMBL cdna:known chromosome:CEL140:X:4732932:4738519:1 gene:F55D10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4733068-4738462(+) 98.84 100.0
Public Domain and Genome References
Gene Title Alpha-mannosidase
Ensembl F55D10.1
Entrez Gene 180749 Entrez gene
SwissProt Q20829 EMBL-EBI
WormBase CE28456 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247129_AT glycosyl hydrolase family 38 protein at
CANINE_2:CFA.21506.1.S1_S_AT similar to Lysosomal alpha-mannosidase precursor (Mannosidase, alpha B) (Lysosomal acid alpha-mannosidase) (Laman) (Mannosidase alpha class 2B member 1) cfa
CANINE_2:CFAAFFX.26400.1.S1_S_AT similar to Lysosomal alpha-mannosidase precursor (Mannosidase, alpha B) (Lysosomal acid alpha-mannosidase) (Laman) (Mannosidase alpha class 2B member 1) cfa
DROSOPHILA_2:1635125_A_AT dm
DROSGENOME1:151851_AT dm
HG-U133A:209166_S_AT mannosidase, alpha, class 2B, member 1 hs
HG-U133_PLUS_2:209166_S_AT mannosidase, alpha, class 2B, member 1 hs
HG-FOCUS:209166_S_AT mannosidase, alpha, class 2B, member 1 hs
HG-U133A_2:209166_S_AT mannosidase, alpha, class 2B, member 1 hs
HUGENEFL:U05572_S_AT mannosidase, alpha, class 2B, member 1 hs
U133_X3P:G1658373_3P_A_AT mannosidase, alpha, class 2B, member 1 hs
HG-U95AV2:34670_AT mannosidase, alpha, class 2B, member 1 hs
MU11KSUBA:U29947_S_AT mannosidase 2, alpha B1 mm
MG-U74AV2:99562_AT mannosidase 2, alpha B1 mm
MG-U74AV2:162150_R_AT mannosidase 2, alpha B1 mm
MG-U74BV2:163968_AT mannosidase 2, alpha B1 mm
MOE430A:1436781_AT mannosidase 2, alpha B1 mm
MOUSE430A_2:1436781_AT mannosidase 2, alpha B1 mm
MOUSE430_2:1436781_AT mannosidase 2, alpha B1 mm
MOE430A:1416340_A_AT mannosidase 2, alpha B1 mm
MOUSE430A_2:1416340_A_AT mannosidase 2, alpha B1 mm
MOUSE430_2:1416340_A_AT mannosidase 2, alpha B1 mm
MU19KSUBA:TC24567_S_AT Mannosidase 2, alpha B1, mRNA (cDNA clone MGC:5884 IMAGE:3488681) mm
RAE230A:1388699_AT mannosidase 2, alpha B1 rn
RAT230_2:1388699_AT mannosidase 2, alpha B1 rn
RG-U34A:RC_AA891670_G_AT mannosidase 2, alpha B1 rn
RG-U34A:RC_AA891670_AT mannosidase 2, alpha B1 rn
RG-U34C:RC_AI233828_AT mannosidase 2, alpha B1 rn
RAE230A:1390012_AT Mannosidase 2, alpha B1 rn
RAT230_2:1390012_AT Mannosidase 2, alpha B1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4559 alpha-mannosidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81731 Hypothetical protein F55D10.1 [Caenorhabditis elegans] ref|NP_508811.1| F55D10.1 [Caenorhabditis elegans] 0.0
blast CAE68642 Hypothetical protein CBG14532 [Caenorhabditis briggsae] 0.0
blast NP_001014354 hypothetical protein LOC541519 [Danio rerio] gb|AAH91843.1| Hypothetical LOC541519 [Danio rerio] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 1.0E-126
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 6.1E-12
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 2.3E-52
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 1.0E-126
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 1.9E-92
Pfam IPR000602 EMBL-EBI Glycoside hydrolase, family 38 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:189740_AT
agagcccaaggcatggtagcctctgacggagttgtggaaattatgcttcatcgtcgttgc
ttttatgatgatcactttggagttgaggaggcattggatgagcctggaaaagatggtgca
gggctggttgccatgggaaaacatatcgttctgtttactgatgtcaaggcatcaacttca
cagcttcgtcctctcgccattgacaacttccatcagccggtgttagcatttaacaaaaat
ccagaaaagtttgaatttaatagactattggagtattctgggctaaaatttgagttgcca
attggaatacatctcctaaccctcgagaaatggcacaagaagagtagccttatcagattt
gagaacatttatcacggtgaagaaggaaatagcattaaagaatttgatccaacacacatt
ttcacaaacttcgatattgtgtcattcaaagagcttctacttggtgctaacagagtagtg
ggaaaaaataaagaatcgggatcgtcattccgcagttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGCCCAAGGCATGGTAGCCTCTG 619 67 2194 Antisense
GGTAGCCTCTGACGGAGTTGTGGAA 290 505 2208 Antisense
ATGCTTCATCGTCGTTGCTTTTATG 46 43 2236 Antisense
GTGCAGGGCTGGTTGCCATGGGAAA 261 479 2309 Antisense
TCGTTCTGTTTACTGATGTCAAGGC 10 605 2339 Antisense
TGTCAAGGCATCAACTTCACAGCTT 612 557 2355 Antisense
ACTTCCATCAGCCGGTGTTAGCATT 166 105 2399 Antisense
ATTGGAATACATCTCCTAACCCTCG 320 3 2494 Antisense
CCAACACACATTTTCACAAACTTCG 367 275 2602 Antisense
AGAGCTTCTACTTGGTGCTAACAGA 306 69 2643 Antisense
AATCGGGATCGTCATTCCGCAGTTT 375 169 2687 Antisense
Sequence Source Affymetrix Proprietary Database